[Induction of anti-hepatocellular carcinoma immunity by Hyper-IL-6 gene in vivo].

Aim: To prepare the mouse hepatocellular carcinoma (HCC) cells with the stable expression of Hyper-IL-6 and explore the possibility of inducing active anti-HCC immune response by Hyper-IL-6 gene.

Methods: Hyper-IL-6 gene was transfected into mouse HCC cells MM45T.Li using Lipofectamine(TM);2000.

[Construction and expression of fusion gene encoding Hyper-IL-6 in mammalian cells].

Aim: To construct and express fusion gene encoding Hyper-IL-6 in mammalian cells.

Methods: Using geneSOEing method, human sIL-6R cDNA was fused with IL-6 cDNA by a linker rich in glycine through PCR technique and cloned into pIRES(2)-EGFP. The constructed plasmid was transfected into mammalian cells.

[The effect of artificial support system on serum cytokine in chronic severe hepatitis].

Objective: To explore the changes of cytokines including TNFalpha, TGFbeta1 and nitrogen monoxide, and endotoxin in the serum of chronic severe hepatitis after the treatment of ALSS, and to evaluate further the value of ALSS in the treatment of chronic severe hepatitis.

Methods: Forty two patients were screened. The changes of TNFalpha, TGFbeta1, nitrogen monoxide and endotoxin were detected respectively.