[Microbial Diversity of Filamentous Sludge Bulking at Low Temperature].

To investigate the characteristics of microbial diversity during filamentous bulking at low temperature, the induction of sludge bulking was successfully carried out using a low-temperature sequencing batch reactor(SBR). With the help of Illumina MiSeq high-throughput sequencing technology, the overall changes in the microbial community structure of activated sludge, the characteristics of each specific microbial community, and the specific genera were all investigated under different sludge sedimentation performances. The results showed that filamentous bulking can be successfully induced after the system operating temperature drops to (14±1)℃, and the COD and TN removal rates can still be maintained at approximately 90% and 86%, respectively, with the sludge volume index deteriorating to 663.

[Realization of Limited Filamentous Bulking with Type 0092 Filamentous Bacteria as the Dominant Filamentous Bacteria in Shortcut Nitrification].

Type 0092 filamentous bacteria generally do not result in excessive sludge bulking. To take advantage of this, domestic sewage was used to inoculate shortcut nitrification sludge in a sequencing batch reactor (SBR). Sludge settleability, the nitrite accumulation ratio (NAR), pollutant removal characteristics, and the dynamic variation of microbial communities during the system startup and maintenance were investigated.

[Stability of Nitritation Combined with Limited Filamentous Bulking Under Intermittent Aeration].

Limited filamentous bulking (LFB) combined with nitritation under low dissolved oxygen (DO) is a new technology for enhancing nitrogen removal and reducing aeration requirements. In order to investigate the feasibility and sustainability of this technology, two sequence batch reactors (SBRs) were operated under different regimes to stimulate different aeration modes under low DO (0.3-0.

A Systems Biology-Based Approach to Uncovering Molecular Mechanisms Underlying Effects of Traditional Chinese Medicine Qingdai in Chronic Myelogenous Leukemia, Involving Integration of Network Pharmacology and Molecular Docking Technology.

BACKGROUND The method of multiple targets overall control is increasingly used to predict the main active ingredient and potential target group of Chinese traditional medicines and to determine the mechanisms involved in their curative effects. Qingdai is the main traditional Chinese medicine used in the treatment of chronic myelogenous leukemia (CML), but the complex active ingredients and antitumor targets in treatment of CML have not been clearly defined in previous studies. MATERIAL AND METHODS We constructed a protein-protein interaction network diagram of CML with 638 nodes (proteins) and 1830 edges, based on the biological function of chronic myelocytic leukemia by use of Cytoscape, and we determined 19 key gene nodes in the CML molecule by network topological properties analysis in a data bank.

[Induction of low dissolved oxygen limited filamentous bulking and optimization of nitrogen and phosphorus removal].

Actual domestic sewage was treated by SBR process operating in Anoxic/Oxic pattern. Limited filamentous bulking was induced by low dissolved oxygen at normal temperature, and SVI was maintained 150-220 mL/g. Occurrence of limited filamentous bulking and growth of microorganism were researched, as well as optimization of nitrogen and phosphorus removal.

[Nitrogen removal by enhanced endogenous denitrification with excess activated sludge reduction technology].

In order to increase the efficiency of nitrogen removal by endogenous denitrification, and to realize the excess activated sludge reduction in the traditional wastewater treatment process, this investigation proposes a hydrolysis/anoxic/oxic (H/A/O) process utilizing nitrogen removal with excess activated sludge reduction. The experimental equipment for continuous treatment of domestic wastewater was set up and the pilot experiment was conducted. The results showed that, without adding external carbon sources and alkalinity, the removal efficiency of COD, NH4+-N and TN were higher than 90%, 95% and 75%, respectively, under the condition that the hydraulic retention time was 10 h and the backflow ratio of nitrification liquor was 300%, the COD of influent between 220-410 mg/L, the NH4+-N of influent between 36-58 mg/L.

[Effect of nutrient on sludge settling property and bulking controls].

The effect of nutrient (N and P) deficiency on activated sludge settleability and characteristics of activated sludge flocs in Sequencing Batch Reactor (SBR) fed with brewery wastewater were investigated. The results showed that the activated sludge had good settling properties at influent BOD5/N/P was 100/5/1,100/5/0.8 and 100/4/0.

[On-line control of external carbon addition to predenitrification process].

With domestic wastewater of low ratio of carbon and nitrogen (C/N), the control of external carbon dosage was studied for the predenitrification process with continuous flow. The objective is to keep the external carbon usage as low as possible while nitrate plus nitrite concentrations (NOx(-)-N) of outflow under demand. The experimental results show that nitrogen removal efficiency couldn't be improved by increasing total recirculating rate with total recirculating rate more than 2 for lack of carbon source and removal efficiency of TN couldn't be largely improved by double dosage of carbon source with constant recirculating rate when NOx(-)-N concentration reaches about 2 mg/L.