[Investigation on infection in liver disease patients in Hangzhou City].

Objective: To understand the infection in liver disease patients in Hangzhou City.

Methods: A total of 1 200 patients with liver diseases were enrolled, including 300 patients with liver cancer, 300 cases with hepatitis B, 300 cases with hepatic fibrosis and 300 cases with fatty degeneration of the liver, while 1 200 healthy people served as controls. The serum anti- IgG and IgM antibodies were detected in the subjects using ELISA assay.

[A sero-epidemiological survey of infections in high-risk populations in Hangzhou City].

Objective: To understand the status of infections and the awareness of toxoplasmosis prevention and control knowledge among high-risk populations in Hangzhou City.

Methods: The serum anti- antibodies were detected in 100 HIV/AIDS patients, 100 cancer patients, 100 pregnant women and 100 healthy controls, and the awareness of toxoplasmosis prevention and control knowledge was investigated using a questionnaire.

Results: The sero-prevalence of infection was 31%, 30% and 21% in HIV/AIDS patients, cancer patients and pregnant women, which was all significantly higher than in healthy controls ( = 14.

Population Pharmacokinetics and Pharmacodynamics of Piperacillin/Tazobactam in Patients with Nosocomial Infections.

Objective: The study was to establish a population pharmacokinetic (PPK) model of piperacillin (PIP) and tazobactam (TAZ) that explain pharmacokinetic variability and to propose optimized dosage regimens in patients with nosocomial infections.

Methods: In total, 310 PIP and 280 TAZ concentration-time points were collected at steady state over multiple dosing intervals from 50 patients who received PIP/TAZ infused within 30 min or over 3 h. Drug analysis was performed by high-performance liquid chromatography (HPLC).

N-palmitoylethanolamine and N-acetylethanolamine are effective in asteatotic eczema: results of a randomized, double-blind, controlled study in 60 patients.

Background: Asteatotic eczema (AE) is characterized by itchy, dry, rough, and scaling skin. The treatments for AE are mainly emollients, usually containing urea, lactic acid, or a lactate salt. N-palmitoylethanolamine (PEA) and N-acetylethanolamine (AEA) are both endogenous lipids used as novel therapeutic tools in the treatment of many skin diseases.

Identification of proteins inducing short-lived antibody responses from excreted/secretory products of Schistosoma japonicum adult worms by immunoproteomic analysis.

Unlabelled: The excretory/secretory antigens of Schistosoma japonicum (Sj ESAgs) play important roles in host-parasite immune interactions. In this study, the antibody response patterns to Sj ESAgs in sera of individual rabbits at the healthy stage, 2-6 weeks post-infection and 4-16 weeks after treatment were examined. Antigens inducing short-lived antibody responses were selected by comparing differences in immune recognition of proteins in sera across the different stages by Western blotting and identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF-MS).

Population pharmacokinetics of digoxin in elderly patients.

This study was aimed at determining the population pharmacokinetics of digoxin and identifying factors that explain pharmacokinetic variability in elderly patients. The data of 142 elderly patients and 448 samples were collected after repetitive oral digoxin. Blood samples were drawn at various times after administration.

Characterization of IgG responses of rabbits to Sj14-3-3 protein after experimental infection with Schistosoma japonicum.

An indirect enzyme-linked immunosorbent assay method was developed for detection of IgG against 14-3-3 protein in sera of rabbits. Rabbits infected with 500 cercariae of Schistosoma japonicum were grouped and the characterization of the IgG responses was observed. For the treated group, the IgG could be detected as early as 2-4 weeks post-infection and then their levels rose rapidly and reached a peak at around 6 weeks.

[Epitope identification of monoclonal antibody 5C6 against 14-3-3 protein of Schistosoma japonicum].

Objective: To identify the epitope of monoclonal antibody (McAb) 5C6 against 14-3-3 protein of Schistosoma japonicum by phage display peptide library.

Methods: The phage display 12-peptide library was screened with purified McAb 5C6 against 14-3-3 protein of S. japonicum three rounds of bio-panning "adsorption-elution-amplification" to enrich the specific binding phages.

Thioredoxin glutathione reductase as a novel drug target: evidence from Schistosoma japonicum.

Background: Schistosomiasis remains a major public health concern affecting billions of people around the world. Currently, praziquantel is the only drug of choice for treatment of human schistosomiasis. The emergence of drug resistance to praziquantel in schistosomes makes the development of novel drugs an urgent task.

[Expression and characterization of recombinant Sj23HD-HSA fusion protein in Saccharomyces cerevisiae].

Objectives: To prepare the fusion protein of large hydrophilic domain of 23 kDa membrane protein of Schistosoma japonicum and the mature peptide of human serum albumin (Sj23HD-HSA) and investigate its immunoreactivity.

Methods: A fusion protein gene encoding Sj23HD-HSA fusion protein was prepared by overlapping PCR, which was confirmed by TA cloning and DNA sequencing. The fusion gene of Sj23HD-HSA was directionally subcloned into yeast expression plasmid pWX530 to construct a recombinant plasmid Sj23HD-HSA/pWX530.

[Expression and characterization of thioredoxin glutathione reductase of Schistosoma japonicum].

Objective: To prepare the recombinant thioredoxin glutathione reductase of Schistosoma japonicum (SjTGR) with biological activity.

Methods: The open reading frame DNA sequence of SjTGR was fused with a bacterial-type selenosysteine insertion sequence (SECIS) element by PCR to form a chimeric gene. The chimeric gene was subcloned into expression plasmid pET-41a to construct a recombinant plasmid SjTGR-pET-41a.

[Preparation and characteristics of Schistosoma japonicum signaling protein 14-3-3].

Objective: To prepare soluble recombinant signal transduction protein 14-3-3 of Schistosoma japonicum (rSj14-3-3) and investigate its immunologic features.

Methods: The cDNA fragment of signal transduction protein 14-3-3 gene was prepared from Schistosoma japonicum adult worm mRNA, and was subcloned to the downstream of glutathione S transferase gene of expression vector pGEX-4T-3 to construct a recombinant expression plasmid 14-3-3/pGEX-4T-3. The recombinant plasmids were transferred into E.

[Application of F-ELISA for immunodiagnosis of schistosomiasis japonica].

Objective: To optimize the experimental conditions of fast enzyme-linked immunosorbent assay (F-ELISA) and evaluate its performance for immunodiagnosis of schistosomiasis japonica.

Methods: HRP-SPA was used at different concentrations in the assay to determine the best HRP-SPA concentration by comparing the detection accuracy. The working conditions of F-ELISA were optimized by examining the ratio of absorbance values of positive and negative controls with different incubation time of serum samples and HRP-SPA.

[Evaluation of early diagnostic value of 6 antigens from Schistosoma japonicum in mice].

Objective: To find out the candidate antigen for immunoreagent, which could be used to diagnose Schistosoma japonicum infection early in mice.

Methods: The mice were infected with cereariae of S. japonicum Chinese mainland strain.

[Characterization and preliminary application of six monoclonal antibodies against recombinant signal protein 14-3-3 of Schistosoma japonicum].

Objectives: To identify the properties of six monoclonal antibodies (McAbs) against recombinant signal protein 14-3-3 of Schistosoma japonicum, and investigate their value in the diagnosis of schistosomiasis.

Methods: The subclasses, titers, affinity-constants, detection limits and specificities of six McAbs were identified by ELISA and Western blotting. Dot Enzyme-linked Immunosorbent Assay (Dot-ELISA) for detecting the 14-3-3 protein in the sera of rabbits infected with Schistosoma japonicum was established, then it was used to observe the dynamics of 14-3-3 protein in sera of rabbits infected with Schistosoma japonicum before and after treatment.

Monitoring specific antibody responses against the hydrophilic domain of the 23 kDa membrane protein of Schistosoma japonicum for early detection of infection in sentinel mice.

Background: Schistosomiasis remains an important public health problem throughout tropical and subtropical countries. Humans are infected through contact with water contaminated with schistosome cercariae. Therefore, issuing early warnings on the risk of infection is an important preventive measure against schistosomiasis.

Detection of the circulating antigen 14-3-3 protein of Schistosoma japonicum by time-resolved fluoroimmunoassay in rabbits.

Background: Schistosomiasis remains a major public health concern that afflicts millions of people worldwide. Low levels of Schistosoma infection require more sensitive diagnostic methods. In this study, a time-resolved fluoroimmunoassay (TRFIA) was developed for detecting the signal transduction protein 14-3-3, a circulating antigen of Schistosoma japonicum.

Kinetics of circulating antigen 14-3-3 in sera of rabbits firstly infected with Schistosoma japonicum and treated with/without praziquantel.

A sandwich ELISA was developed for the detection of circulating antigen 14-3-3 in the sera of rabbits. Rabbits that were infected with 500 cercariae of Schistosoma japonicum were grouped and the kinetics of 14-3-3 was observed. For the treated group, the 14-3-3 protein could be detected as early as 2-4 weeks postinfection and then its levels rose rapidly and reached a peak at around 6 weeks.