Anti-Melanoma Activity of Single Intratumoral Injection of ZnPc Micelles Mixed With in situ Gel in B16 Bearing Mouse.

Photodynamic therapy (PDT) is a potential treatment strategy for melanoma. As a second-generation photosensitizer, Zinc phthalocyanine (ZnPc) has many advantages for anti-tumor PDTs, such as strong absorption in the red and near infrared regions, high photo and chemical stability, etc. However, ZnPc has a poor water solubility and is apt to aggregate due to the π-π interaction between molecules, which limits its applications.

Krüppel-like factor 12 regulates aging ovarian granulosa cell apoptosis by repressing SPHK1 transcription and sphingosine-1-phosphate (S1P) production.

Oxidative stress triggered by aging, radiation, or inflammation impairs ovarian function by inducing granulosa cell (GC) apoptosis. However, the mechanism inducing GC apoptosis has not been characterized. Here, we found that ovarian GCs from aging patients showed increased oxidative stress, enhanced reactive oxygen species activity, and significantly decreased expression of the known antiapoptotic factor sphingosine-1-phosphate/sphingosine kinase 1 (SPHK1) in GCs.

Treatment of ammonia-embodied wastewater by a transition-metal-based photochemical catalysis strategy.

Inspired by the self-purification process and a low nitrogen content of the ocean, and the fact that the driving-force behind ecological cycle is solar irradiation, a novel photochemical strategy was designed to spontaneously remove inorganic ammonia nitrogen from wastewater with solar irradiation. This strategy is based on the principles of green chemistry and energy efficiency, and meanwhile the prevention from the introduction of accompanying pollution. In our strategy, a photo-Fe (or Mn)-O system was built to remove ammonia-nitrogen from its aqueous solution.

3D semiconducting Co-MOFs based on 5-(hydroxymethyl)isophthalic acid and imidazole derivatives: syntheses and crystal structures.

Two three-dimensional cobalt-based metal-organic frameworks with 5-(hydroxymethyl)isophthalic acid (HHIPA), namely poly[[μ-1,4-bis(2-methyl-1H-imidazol-1-yl)benzene-κN:N][μ-5-(hydroxymethyl)isophthalato-κO:O]cobalt(II)], [Co(CHO)(CHN)] (1), and poly[tris[μ-1,4-bis(1H-imidazol-1-yl)benzene-κN:N]bis[μ-5-(hydroxymethyl)isophthalato-κO:O:O]dicobalt(II)], [Co(CHO)(CHN)] (2), were synthesized under similar hydrothermal conditions. Single-crystal X-ray diffraction analyses revealed that 5-(hydroxymethyl)isophthalate (HIPA) and 1,4-bis(2-methyl-1H-imidazol-1-yl)benzene (1,4-BMIB) are simple linkers connecting cobalt centres to build a fourfold interpenetration dia framework in complex 1. However, complex 2 is a pillared-layer framework with a (3,6)-connected network constructed by 1,4-bis(1H-imidazol-1-yl)benzene (1,4-DIB) linkers, 3-connected HIPA ligands and 6-connected Co centres.

Increased PIT1 and PIT2 Expression in Streptozotocin (STZ)-induced Diabetic Mice Contributes to Uptake of iAs(V).

Objective: This study aimed to investigate the susceptibility of mice with streptozotocin(STZ)-induced diabetes mellitus (TIDM) to the uptake of pentavalent inorganic arsenic (iAsV) and the possible molecular mechanism.

Methods: TIDM was induced in mice by STZ. TIDM and normal mice were treated with 15.

Cardioprotective effect of valsartan in mice with short-term high-salt diet by regulating cardiac aquaporin 1 and angiogenic factor expression.

Hypertension is the most common risk factor for various cardiovascular and cerebrovascular diseases that affects approximately 61 million, or 25% of the population in United States. The dietary salt intake is one of the most important but modifiable factors for hypertension. In the current study, we aim to elucidate the role of aquaporin 1 in high-salt-induced hypertension and cardiac injuries and whether angiotensin II receptor blocker valsartan could ameliorate the effect of high salt on blood pressure.

[Effect of mesenchymal stem cell transplantation on immunological injury of the ovary in mice].

Objective: To investigate the effect of mesenchymal stem cell (MSC) transplantation in repairing ovarian injury in mice sensitized with porcine ovarian proteins.

Methods: Wild-type female mice with ICR background (6-8 weeks old) were divided randomly into groups A, B and C (n=12). In groups B and C, the mice were treated with the total protein extract from porcine ovary to induce immunological injury of the ovary, while those in group A received no treatment.

[Comparison of three methods for evaluating acrosome reaction in human spermatozoa].

Objective: To find a convenient and exact method for evaluating acrosome reaction in human spermatozoa.

Methods: The semen of the normal male was mixed and then divided into 6 groups. Coomassie brilliant blue (CBB) staining, chlortetracycline (CTC) fluorescence staining and acid phosphatase (ACP) detection were used for morphological observation and data analysis of the acrosome status of the human sperm treated with or without progesterone.

[Expression of recombinant human zona pellucida-3 protein (rhZP3) in Pichia pastoris].

Human Zona Pellucida(ZP), which is a complex matrix surrounding oocytes,is comprised of three immunologically distinct glycoproteins(hZP1, hZP2 and hZP3). Because hZP3 possesses the sperm receptor activity and the acrosome-inducing activity, it has long been used as a candidate antigen to develop an immunocontraceptive vaccine. However, a large amount of native hZP3 protein is unavailable.