[Lentivirus-mediated BMP-2 overexpression plasmid transfection into bone marrow mesenchymal stem cells combined with silk fibroin scaffold for osteoblast transformation].

Objective: To explore the effect of lentivirus-mediated BMP-2 overexpression plasmid transfection into bone marrow mesenchymal stem cells and silk fibroin scaffold on osteoblast transformation.

Methods: The lentivirus BMP-2 overexpression vector was constructed, bone marrow mesenchymal stem cells were cultured, and the combined culture system of nuclear scaffolds was constructed. Alizarin red staining and alkaline phosphatase staining were used to detect the osteogenic transformation of bone marrow mesenchymal stem cells in vitro.

[Spectral study of the effects of glasses lenses' color depth on stereoscopic vision].

Glasses with zero focal-length lens and different shades of red, yellow and blue were studied in terms of absorbance by UV-Vis spectral absorption method. The effect of lens with different shades of the same color on stereoscopic vision was comparatively studied from the medical view. The study has shown that, for yellow and blue lens, there is no difference in the absorbance between the deep-color and light-color lens in UV; at 550 nm, the absorbance of deep-color lens is higher than that of light-color lens.