Effects of matrix, electrospray solution, and laser light on the desorption and ionization mechanisms in electrospray-assisted laser desorption ionization mass spectrometry.

Electrospray-assisted laser desorption ionization (ELDI) is a technique which combines laser desorption with subsequent electrospray ionization. It is useful for directly detecting small and large molecules in solid or liquid samples under ambient conditions. In this paper, the detection of the protein molecules desorbed on a dry protein spot by using pulse laser energies of up to 300 microJ was demonstrated.