[Relationship between morphology and pathogenicity of Blastocystis hominis trophozoites].

Six hundred and eighty-six fresh fecal specimens were collected from outpatients (663 well-formed feces and 23 watery feces) during March 2011 to March 2012. All specimens were examined microscopically by direct smear and iodine stained method. B.

[Cloning, expression and identification of surface antigen SAG4 of Toxoplasma gondii].

Objective: To clone and express surface antigen SAG4 gene of Toxoplasma gondii, and analyze its immunoreactivity.

Methods: Specific primers were designed based on the reported SAG4 gene of T. gondii RH strain (GenBank Accession No: AF340224.

[A preliminary study on preservation methods of Blastocystis hominis].

Objective: To observe the effect of different cryoprotective agents and temperature factors on the viability of Blastocystis hominis so as to explore the ideal method for preservation of B. hominis.

Methods: B.

[Observation on polytene chromosomes of the ovarian nurse cell of anopheles minimus from Yuanjiang].

Anopheles minimus collected from Yuanjiang, Yunnan Province, were bred with standard methods in lab. The ovarian nurse cells of A.minimus were separated and stained, and the whole polytene chromosomes were photographed under light microscope and compared with A.