mutation predicts unfavorable treatment-free survival in Chinese chronic lymphocytic leukemia patients.

Background: Splicing factor 3b subunit 1 (), a splicing factor modulating RNA alternative splicing, is frequently mutated in multiple hematological malignancies including myelodysplastic syndromes and chronic lymphocytic leukemia (CLL). The clinical impact of mutation on CLL remains controversial especially for patients of Asian descent.

Methods: We retrospectively analyzed the frequency of mutation by Sanger sequencing in 399 newly diagnosed Chinese CLL patients.

Analysis of the miRNA-mRNA networks in malignant transformation BEAS-2B cells induced by alpha-particles.

The aim of this study was to determine the toxicity induced by irradiation with alpha-particles on malignant transformation of immortalized human bronchial epithelial cells (BEAS-2B) using miRNA-mRNA networks. The expression of BEAS-2B cells was determined by measuring colony formation, mtDNA, mitochondrial membrane potential (MMP), and ROS levels. Changes in BEAS-2B cell gene expression were observed and quantified using microarrays that included an increase in 157 mRNA and 20 miRNA expression and a decrease in 77 mRNA and 48 miRNA.

Norcantharidin induced DU145 cell apoptosis through ROS-mediated mitochondrial dysfunction and energy depletion.

Norcantharidin (NCTD), a demethylated analog of cantharidin derived from blister beetles, has attracted considerable attentions in recent years due to their definitely toxic properties and the noteworthy advantages in stimulating bone marrow and increasing the peripheral leukocytes. Hence, it is worth studying the anti-tumor effect of NCTD on human prostate cancer cells DU145. It was found that after the treatment of NCTD with different concentrations (25-100 μM), the cell proliferation was significantly inhibited, which led to the appearance of micronucleus (MN).

Studies on Etching and Damage Action of Low Energy Ion Beam on icrobial Cells.

The action of 20 keV N(+) ion on Deinococcus radiodurans and E. coli was investigated by means of scanning electron microscope(SEM) and electron spin resonance(ESR). The results showed that ion implantation exerted direct etching damage and indirect free radicals action by energy deposition on the cells of the two microbes.

Reverse Oxygen Effect on gamma-Irradiation Induced Single Strand Breaks of Plasmid DNA.

Oxygen effect on gamma-irradiation induced single strand breaks (SSB) of plasmid DNA was measured by the method of gel scanning. It was found that, in the presence of mannitol, the yield of SSB in N(2)-saturated DNA solution was larger than that in air-saturated solution so that the oxygen enhancement ratio (OER) of SSB became smaller than 1, which indicates the appearance of reverse oxygen effect of DNA SSB. At the same time, G(SSB) and its OER decreased with increasing concentration of mannitol.

Mechanisms of Formation of DNA Strand Breaks Induced by gamma-Rays.

Dose responses of DNA single- and double-strand breaks (SSB and DSB) of pBR322 irradiated with gamma-rays were measured by gel electrophoresis scanning and the production process of SSB was tested. Results showed that the formation of DSB was related to the free radicals scavenging capacity sigma of mannitol dissolved in DNA solution. The ratio of alphaDSB to betaDSB non-linearly increased with increasing sigma.

Kinetics of Ionizing Irradiation-induced DNA Strand Breaks.

By measuring the dose effect of ionizing irradiation-induced single- and double-strand breaks (SSB and DSB) of pBR322, the responses of the yields of SSB and DSB to DNA concentrations were obtained. It was found that, in the presence of mannitol, the reciprocals of G(SSB) and G(alphaDSB) had a linear relationship to the reciprocal of DNA concentration so that the competition reaction of DNA and mannitol to.OH radicals could be described by a second-order kinetics.