Two new species of (Diaporthaceae, Diaporthales) in China.

Species of have been reported as plant endophytes, pathogens and saprobes on a wide range of plant hosts. Strains of were isolated from leaf spots of and dead culms of in China, and identified based on morphology and molecular phylogenetic analyses of combined internal transcribed spacer region (ITS), calmodulin (), histone H3 (), translation elongation factor 1-alpha () and β-tubulin () loci. As a result, two new species named and are identified, described and illustrated in the present study.

Diversity of Species from Fagaceous Leaf Spots in China and Pathogenicity for Chinese Chestnut.

Pestalotioid fungi have been frequently studied with respect to their morphology, molecular phylogeny, and pathogenicity. is a pestalotioid genus that is morphologically characterized by 5-celled conidia with single apical and basal appendages. In the present study, fungal isolates were obtained from diseased leaves of hosts in China in 2016 to 2021 and identified based on morphology and phylogenetic analyses of the 5.

 gen. et sp. nov. (Tubakiaceae, Diaporthales) from leaf spots in China.

Tubakiaceae represents a distinct lineage of Diaporthales, including its type genus and nine additional known genera. Tubakiaceous species are commonly known as endophytes in leaves and twigs of many tree species, but can also be plant pathogens causing conspicuous leaf symptoms. In the present study, isolates were obtained from diseased leaves of collected in Guangdong Province, China.

Additions to The Knowledge of (, ) in China.

The species of Tubakia (Tubakiaceae, Diaporthales, Sordariomycetes) have often been reported as endophytes and pathogens on woody plants. During the investigation of Tubakia species from Fagaceae trees in China, 46 isolates were obtained from diseased leaves and seeds. The characterization of these isolates was based on the observation of morphological characters, the effect of temperature on mycelial growth rate, as well as the combined genes of ITS, tef1 and tub2.

Morphology and Phylogeny of (, ) from Leaves in China.

is a family of flowering plants widely distributed in the Northern Hemisphere, including deciduous and evergreen trees and shrubs. Species of are well-known agents of leaf spot diseases, but targeted sampling on is still missing. To determine the diversity of species associated with leaf spot in China, investigations were conducted in the main areas of distribution from 2016 to 2021.

Rhizobium quercicola sp. nov., isolated from the leaf of Quercus variablis in China.

Strain DKSPLA3, a novel Gram-negative, catalase-positive, oxidase-positive, non-spore-forming, aerobic, non-nitrogen-fixing, non-motile bacterium was isolated from Quercus variablis leaf, in Zunyi, Guizhou, China. Growth occurred at 4-37 °C (optimum 28 °C), pH 4.0-9.

Pinirhizobacter soli gen. nov., sp. nov., a novel low temperature resistant gammaproteobacterium in the family Rhodanobacteraceae isolated from rhizospheric soil of Larix gmelinii.

Three yellow-colored strains, NC2-4-308, NC3-4-326 and NA3-4-109, were isolated from the rhizosphere soil of Larix gmelinii in Nanwenghe Nature Reserve, Great Khingan, China. These strains were oxidase- and catalase-positive and Gram-staining-negative. The cells were non-motile short rods that were aerobic and non-spore-forming.

Two new species of (, ) associated with tree cankers in the Netherlands.

(, ) is a common fungal genus inhabiting plant tissues as endophytes, pathogens and saprobes. Some species are reported from tree branches associated with canker diseases. In the present study, samples were collected from , and in Utrecht, the Netherlands.

Morphology and Phylogeny of (, ) from Leaves in China.

(, ) is a well-classified genus inhabiting leaves, branches and fruits of the hosts in three plant families, namely , and . In the present study, eighteen isolates were obtained from diseased leaves of hosts collected from Fujian, Guangdong, Hainan, Henan, Jiangxi and Shaanxi provinces in China. Morphology from the cultures and phylogeny based on the 5.

Affinibrenneria salicis gen. nov. sp. nov. isolated from Salix matsudana bark canker.

L3-3HA, a Gram-negative-staining, facultatively anaerobic, motile bacterial strain, was isolated from the symptomatic bark of Salix matsudana canker in China. 16S rRNA gene analysis revealed that the novel strain shares the highest sequence similarity with Brenneria goodwinii FRB141 (95.5%).

sp. nov., isolated from air.

A Gram-stain-negative, strictly aerobic, non-spore-forming, rod-shaped, motile with polar flagella and pale-orange bacterium, designated strain 122213-3, was isolated from air, collected at the foot of the Xiangshan Mountain, located in Beijing, PR China. Optimal growth occurred at 28 °C, at pH 7 and in the presence of 0-1 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that 122213-3 clustered with species of the genus and formed a distinct sublineage, showing highest similarities to CC-AFH3 (96.

Stenotrophomonas cyclobalanopsidis sp. nov., isolated from the leaf spot disease of Cyclobalanopsis patelliformis.

A Gram-negative, facultatively anaerobic, motile bacterial strain, TPQG1-4, was isolated from the leaf of Cyclobalanopsis patelliformis with spot disease. The isolate was investigated using the polyphasic taxonomic approach. 16S rRNA gene sequencing and analyzing revealed that the novel strain shares the highest sequence similarity with Stenotrophomonas lactitubi M15 (99.

Azohydromonas aeria sp. nov., isolated from air.

A grey pink colored bacterium, strain t3-1-3, was isolated from the air at the foot of the Xiangshan Mountain in Beijing, China. The cells are aerobic, Gram-stain-negative, non-spore-forming, motile and coccoid-rod shaped (0.9-1.

gen. nov., sp. nov., a member of the family , isolated from symptomatic bark of Populus × .

One Gram-negative aerobic bacterial strain was isolated from the bark tissue of  and investigated using a polyphasic approach including 16S rRNA gene sequencing and both phenotypic and chemotaxonomic assays. The 16S rRNA gene and housekeeping gene phylogenies suggest that the novel isolate is different from the other genera of the family The G+C content, major fatty acids, physiological and biochemical data supported the distinctiveness of the novel strain from reference species. The major fatty acids detected in the novel isolate were Cω7 and/or Cω6, C, C 3OH and/or CisoI and Cω7.

Sphingobacterium corticibacter sp. nov., isolated from bark of Populus × euramericana.

One Gram-stain negative, aerobic, non-motile bacterial strain, 2c-3, was isolated from symptomatic canker bark tissue of Populus × euramericana. It was studied by the genome sequence-derived average nucleotide identity (ANI), phylogenetic analysis based on 16S rRNA gene sequences and phenotypic characteristics. 16S rRNA gene data revealed that the novel isolate shares the greatest sequence similarity to Sphingobacterium populi 7Y-4 (97.

Ancrocorticia populi gen. nov., sp. nov, isolated from the symptomatic bark of Populus × euramericana canker.

A Gram-staining positive facultative anaerobic, non-motile strain, sk1b4 , was isolated from canker of symptomatic bark tissue of a Populus × euramericana. 16S rRNA gene sequence analyses showed that strain sk1b4 shared the highest similarity with Arcanobacterium phocisimile (94.1%).

Herbaspirillum piri sp. nov., isolated from bark of a pear tree.

A Gram-stain-negative, aerobic, motile bacterial strain, shQ-4, was isolated from a pear tree in Henan Province, China. The strain grew at 10-41 °C, at pH 4.0-8.

Sphingomonas aeria sp. nov., isolated from air.

A Gram-negative, aerobic, non-spore-forming, non-motile, yellow-pigmented and rod-shaped bacterial strain, designated B093034, was isolated from air at the foot of Xiangshan mountain, located in Beijing, China. Cells of strain B093034 were oxidase-negative and catalase-positive. Growth was observed at 4-41 °C, at pH 4.

Aureimonas populi sp. nov., isolated from poplar tree bark.

Two novel bacterial strains (4M3-2 and 10-107-7) were isolated from poplar tree bark. The strains were Gram-stain-negative facultative aerobes, and produced short rods that were motile because of polar flagella. A phylogenetic tree was reconstructed based on 16S rRNA gene sequences indicating that the two novel strains are related to species of the genus Aureimonas and Aurantimonas.

Sphingobacterium corticis sp. nov., isolated from bark of Populus × euramericana.

A Gram-stain negative, aerobic, non-motile bacterial strain, 23D10-4-9, was isolated from symptomatic canker bark tissue of Populus × euramericana. The isolate grew between 4 and 35 °C, with optimal growth occurring at 25 °C. The species was positive for catalase and negative for oxidase activity.

Brenneria populi subsp. brevivirga subsp. nov. isolated from symptomatic bark of Populus × euramericana canker, and description of Brenneria populi subsp. populi subsp. nov.

Two Gram-stain-negative, facultatively anaerobic, motile bacterial strains isolated from symptomatic bark of Populus × euramericana canker in China were investigated using a polyphasic approach, including 16S rRNA gene sequencing, multilocus sequence analysis, and biochemical and physiological assays. 16S rRNA gene sequencing indicated that these strains belonged to the genus Brenneria, family Pectobacteriaceae, and had the highest sequence similarity with Brenneria populi CFCC 11963T (98 %). DNA-DNA hybridization experiments revealed DNA-DNA relatedness values of 72.

Paracoccus aerius sp. nov., isolated from air.

Strain 011410T, isolated from air at the foot of Xiangshan Mountain, Beijing, China, was Gram-reaction-negative, facultatively anaerobic, oval-shaped, motile with two flagella and catalase- and oxidase-positive. Growth of strain 011410T was observed at 4-41 °C (optimum, 30 °C), at pH 4.5-10.