Whey Protein-Based Hydrogel Microspheres for Endovascular Embolization.

Transarterial embolization (TAE) is an image-guided, minimally invasive procedure for treating various clinical conditions by delivering embolic agents to occlude diseased arteries. Conventional embolic agents focus on vessel occlusion but can cause unintended long-term inflammation and ischemia in healthy tissues. Next-generation embolic agents must exhibit biocompatibility, biodegradability, and effective drug delivery, yet some degradable microspheres degrade too quickly, leading to the potential migration of fragments into distal blood vessels causing off-target embolization.

Construction of the KNOX-BELL interaction network and functional analysis of CmBLH2 under cold stress in Chrysanthemum morifolium.

The three-amino-acid-loop-extension (TALE) homeodomain transcription factor family, including the KNOX and BELL subfamilies, is one of the largest gene families in plants. This family encodes plant-specific transcription factors that play critical roles in regulating plant growth, development, and stress responses. However, their interaction network, as well as resistant functional mechanism in is rarely reported.

Tetraphenylmethane based three-dimensional conjugated microporous polymer as adsorbent for solid-phase extraction of trace sulfonamide antibiotics in milk samples.

Sulfonamide antibiotics have a broad spectrum of antibacterial action and are widely used, but their overuse poses a threat to human health. In this study, a three-dimensional conjugated microporous polymer, which was designated as TPM-CMP, was synthesized via Friedel-Crafts reaction by using tetraphenylmethane (TPM) and biphenyl dichlorobenzene as monomers, and it was utilized as an adsorbent in solid-phase extraction (SPE) of sulfonamides. The TPM-CMP demonstrated high extraction efficiency for sulfonamides due to π-stacking interactions, hydrophobic forces, and pore-filling effects.

A motor imagery classification model based on hybrid brain-computer interface and multitask learning of electroencephalographic and electromyographic deep features.

Objective: Extracting deep features from participants' bioelectric signals and constructing models are key research directions in motor imagery (MI) classification tasks. In this study, we constructed a multimodal multitask hybrid brain-computer interface net (2M-hBCINet) based on deep features of electroencephalogram (EEG) and electromyography (EMG) to effectively accomplish motor imagery classification tasks.

Methods: The model first used a variational autoencoder (VAE) network for unsupervised learning of EEG and EMG signals to extract their deep features, and subsequently applied the channel attention mechanism (CAM) to select these deep features and highlight the advantageous features and minimize the disadvantageous ones.

[Literature research and discussion on properties of a new oversea-introduced traditional Chinese medicine Malaysian ginseng(Tongkat Ali)].

Tongkat Ali(Eurycoma longifolia Jack.) is a medicinal plant of Simaroubaceae in Southeast Asia, with centuries of medicinal history. Rich clinical and pharmacological research results have been attained for this plant, which demonstrates diverse and definite effects and high safety, showcasing advantages and broad market prospects to be introduced as a new medicinal plant.

Transcriptomic Analysis Reveals the Potential Mechanism of Hook.f. & Thomson in Lowering Serum Uric Acid by Reducing Inflammatory State Through CCR7 Target.

Hook.f. & Thomson (CC) is a traditional medicinal herb with multiple biological activities.

Causal mechanisms of quadruple networks in pediatric bipolar disorder.

Background: Pediatric bipolar disorder (PBD) is characterized by abnormal functional connectivity among distributed brain regions. Increasing evidence suggests a role for the limbic network (LN) and the triple network model in the pathophysiology of bipolar disorder (BD). However, the specific relationship between the LN and the triple network in PBD remains unclear.

Nitrogen-doped hollow quadruple-shelled carbon-silicon nanosphere as a solid-phase microextraction adsorbent for the extraction of 19 polycyclic aromatic hydrocarbons in milk samples.

In this study, nitrogen-doped hollow multi-shelled carbon-silicon nanospheres (NSiC-Homs-NS) were prepared using a template method and employed as a coating material for solid-phase microextraction (SPME) fibers. The extraction efficiency of single-, double-, triple-, and quadruple-shelled NSiC-Homs-NS coated fibers for polycyclic aromatic hydrocarbons (PAHs) were investigated, respectively. The NSiC-Homs-NS based SPME fiber coupled with gas chromatography-mass spectrometry was applied to detect 19 PAHs in milk samples.

High-Efficiency Broad-Spectrum Antibacterial Activity of Chitosan/Zinc Ion/Polyoxometalate Composite Films for Water Treatment.

The development of multifunctional films with rapidly killing microorganisms and adsorbing residual antibiotics in wastewater remains a challenging endeavor. In this work, the chitosan/zinc ion/polyoxometalate (CS/Zn/POM) multifunctional films were prepared by the freeze-drying method using chitosan, ZnO, and POM. Notably, the CS/Zn/POM films exhibited excellent bactericidal properties against Gram-positive/negative bacterial strains including (, 99.

Prime editing enables precise genome modification of a hybrid.

Unlabelled: CRISPR/Cas-based genome editing has been extensively employed in the breeding and genetic improvement of trees, yet precise editing remains challenging in these species. Prime editing (PE), a revolutionary technology for precise editing, allows for arbitrary base substitutions and the insertion/deletion of small fragments. In this study, we focused on the model tree poplar 84K ( × ).

Effects of immersion with electro-activated alkaline water on gel properties, biochemicals and odor characteristics of myofibrillar proteins.

This study aimed to investigate the effects of alkaline water electrolysis (AWE) - assisted washing on gelling properties, microstructure, protein conformation, and flavor composition of golden pompano surimi. The experimental results showed that rinsing yield, water holding capacity (WHC), gel strength, and textural properties were effectively improved with AWE for 1 and 3 min. The rheological properties indicated a highly elastic gel with a dense and compact microstructure after soaking in AWE for 1 and 3 min, indicating that short-term alkaline immersion enhances cross-linking between myofibrillar proteins.

Integrating scRNA-seq and Visium HD for the analysis of the tumor microenvironment in the progression of colorectal cancer.

Background: Colorectal cancer (CRC) development is a complex, multi-stage process, transitioning from normal to adenomatous tissue, and then to invasive carcinoma. Despite research, there's a knowledge gap on using high-resolution spatial omics to understand CRC's tumor microenvironment dynamics.

Methods: We used single-cell transcriptomics to study major biological changes and cell interactions in CRC progression.

Joint probabilistic modeling approach for harmonic and three-phase unbalanced disturbance sources.

Harmonics and three-phase imbalance are two common disturbances in power systems that interact with and affect each other, leading to more complex impacts on power system performance. To comprehensively and accurately analyze the distribution and interaction mechanisms of harmonics and three-phase imbalance, this study investigated the joint emission level of harmonic and three-phase unbalanced disturbance sources (H-TU-DS). This paper proposes a joint probabilistic emission level modeling approach.

Elevated systemic inflammation response index is associated with poor outcomes in minor ischemic stroke.

Objectives: Patients with minor ischemic stroke (MIS) have substantial disability rates at 90 days. Our study aimed to explore the association between the systemic inflammation response index (SIRI) and 3-month functional outcomes in patients with MIS.

Methods: We conducted a prospective observational study in patients with MIS [defined as a National Institutes of Health Stroke Scale (NIHSS) score of 0-3] admitted within 24 h from symptoms onset.

Design of sulfonic group based hyper cross-linked polymer as adsorbent for efficient enrichment of endocrine disrupters.

The necessity for the development of effective adsorbents to capture endocrine disrupting chemicals (EDCs) from environmental and food samples arises from the potential health hazards that the EDCs may impose on human beings. Hence, a novel sulfonic group based hyper cross-linked polymer (HQSA-PLE-HCP) was synthesized and explored in solid phase extraction for the enrichment of some EDCs. The HQSA-PLE-HCP demonstrated an excellent adsorption capability for some EDCs (bisphenol F, bisphenol A, bishydroxyphenylbutane and p-tert-butylphenol).

Vision guides the twilight search for oviposition sites of the Asian tiger mosquito, Aedes albopictus.

Background: Oviposition site selection is an important component of vector mosquito reproductive biology. The Asian Tiger mosquito, Aedes albopictus, is a major and important vector of arboviruses including Dengue. Previous studies documented the preference of gravid females for small, dark-colored water containers as oviposition sites, which they sought during the twilight period (dusk) of their locomotor activity.

A Novel Poly(ε-Caprolactone)-Based Photo-Crosslinkable Liquid Copolymer as a Versatile Drug Delivery Platform.

: Hydrophobic semi-solid or liquid biodegradable polymers have shown unique advantages as injectable matrices for sustained release of a wide range of drugs. Here we report the design, synthesis, and characterization of a new low-melt liquid copolymer based on poly(ε-caprolactone) (PCL) and establish its utility as a versatile delivery platform. : The copolymer, mPA20, consisting of short PCL blocks connected via acid-labile acetal linkages, was synthesized using a one-pot reaction and its properties were comprehensively characterized.

Wearable Electrochemical Biosensors for Advanced Healthcare Monitoring.

Recent advancements in wearable electrochemical biosensors have opened new avenues for on-body and continuous detection of biomarkers, enabling personalized, real-time, and preventive healthcare. While glucose monitoring has set a precedent for wearable biosensors, the field is rapidly expanding to include a wider range of analytes crucial for disease diagnosis, treatment, and management. In this review, recent key innovations are examined in the design and manufacturing underpinning these biosensing platforms including biorecognition elements, signal transduction methods, electrode and substrate materials, and fabrication techniques.

Impact of periampullary diverticula on the rates of successful cannulation and ERCP complications: An up-to-date systematic review and meta-analysis.

Objectives: Periampullary diverticulum (PAD) is usually incidentally discovered during abdominal imaging, gastrointestinal endoscopy, and endoscopic retrograde cholangiopancreatography (ERCP). The influence of PAD on ERCP outcomes is unclear. The aim of this systematic review and meta-analysis was to provide an up-to-date evaluation of the impact of PAD on cannulation and ERCP-related complications.

Developing a novel pyridine-based ionic porous organic polymer with functional group for highly efficient extraction of phenolic endocrine disruptors in natural water and tilapia fish.

A pyridine-based ionic porous organic polymer bearing hydroxyl groups (OH-iPOP) was developed for the first time by quaternization and crosslinking of 4-pyridinemethanol with 4,4'-bis(chloromethyl)biphenyl. OH-iPOP exhibited strong adsorption capacity for endocrine disruptors (EDs) including bisphenol A, bisphenol F, p-tert-butylphenol and bisphenol B (82.59-183.

GRK2 mediates cisplatin-induced acute liver injury via the modulation of NOX4.

Background: The present study investigated the function of G protein-coupled receptor kinase 2 (GRK2) in acute liver injury (ALI) by cisplatin, and investigated the protective effect of pharmacological inhibition of GRK2.

Methods: ALI models were generated in global adult hemizygous (ALI-Grk2) mice and wild-type (WT) mice. Liver biochemistry parameters and histopathology were used to evaluate the severity of ALI and the protective effect of pharmacological inhibition of GRK2.

Enhancing peroxidase activity of NiCoO nanoenzyme by Mn doping for catalysis of CRISPR/Cas13a-mediated non-coding RNA detection.

CRISPR/Cas13a with precise and controllable programming of endonuclease activity has been served as powerful tool for RNA sensing. Although with high sensitivity, existing CRISPR/Cas13a-based biosensors need complex amplification procedure or special equipment that limited quantification capability. Here, Mn-doped NiCoO (Mn/NiCoO) nanozyme with enhanced peroxidase activity was synthesized and combined with CRISPR/Cas13a-based reaction to develop a simple, sensitive and universal biosensor for RNA detection, which is achieved through target recognition that activates Cas enzymes to cleave RNA reporter for inhibiting Mn/NiCoO nanozyme to assemble on microplate.

Cas9-PE: a robust multiplex gene editing tool for simultaneous precise editing and site-specific random mutation in rice.

In molecular design breeding, the simultaneous introduction of desired functional genes through specific nucleotide modifications and the elimination of genes regulating undesired phenotypic traits or agronomic components require advanced gene editing tools. Due to limited editing efficiency, even with the use of highly precise editing tools, such as prime editing (PE), simultaneous editing of multiple mutation types poses a challenge. Here, we replaced Cas9 nickase (nCas9) with Cas9 to construct a Cas9-mediated PE (Cas9-PE) system in rice.