Computed tomographic enterography adds value to colonoscopy in differentiating Crohn's disease from intestinal tuberculosis: a potential diagnostic algorithm.

Background: Crohn's disease and intestinal tuberculosis (ITB) are chronic granulomatous disorders that are difficult to distinguish. Computed tomographic enterography (CTE) yields striking findings for Crohn's disease in the small bowel but its role in differentiating Crohn's from ITB is undefined. This prospective study aimed to investigate the value of CTE for differential diagnosis between Crohn's disease and ITB.

Lactobacillus crispatus M206119 exacerbates murine DSS-colitis by interfering with inflammatory responses.

Aim: To investigate the role of Lactobacillus crispatus (L. crispatus) strain China Center for Type Culture Collection (CCTCC) M206119 in intestinal inflammation.

Methods: Forty 8-wk-old Balb/c mice (20 ± 2 g) were divided into four groups of 10 mice each.

Therapeutic effects of four strains of probiotics on experimental colitis in mice.

Aim: To investigate the therapeutic effects of four strains of probiotics (E. feacalis, L. acidophilus, C.

Targeting specificity and pharmacokinetics of asialoorosomucoid, a specific ligand for asialglycoprotein receptor on hepatocyte.

Objective: To testify that the asialoorosomucoid (ASOR) prepared by us has liver-targeting specificity and to investigate its pharmacokinetic characteristics.

Methods: The distribution of 125I-ASOR in vivo was determined by single photon emission computed tomography (SPECT) and immunohistochemical technique after 125I-ASOR was injected into Sprague-Dawley (S-D) rats through their caudal veins. In vitro, different doses of pEGFP-N1 plasmid were transfected into both HepG2 cells and HT1080 cells with the use of ASOR-poly-L-lysine.

Primary shunt hyperbilirubinaemia in a large four-generation family confirming autosomal dominant genetic disorder.

Aim: To describe the pattern of inheritance and confirm the diagnostic criteria of primary shunt hyperbilirubinaemia (PSH).

Methods: Forty members of a family pedigree across four generations were included in this study. All family members were interviewed and investigated by physical examination, hematology and liver function test and the pattern of inheritance was analyzed.

Modulation of the molecular conformation of a hepatocyte-targeting gene drug in order to improve its expression efficiency in vitro.

Aim: To construct different conformations of a plasmid DNA/vector complex (pcDNA3.1/IFN-gamma-ASOR-PLL) and transfect cells of the hepatoma cell line BEL7402 to investigate the optimal conformation of the complex for improved expression efficiency in the target cell.

Methods: Double-distilled water and adjuvant were added to the naked pcDNA3.

Proteomics to display tissue repair opposing injury response to LPS-induced liver injury.

Aim: To examine the protein expression alterations in liver injury/repair network regulation as a response to gut-derived lipopolysaccharide (LPS) treatment, in order to anticipate the possible signal molecules or biomarkers in signaling LPS-related liver injury.

Methods: Male BALB/c mice were treated with intra-peritoneal (i.p.

Study on relationship between expression level and molecular conformations of gene drugs targeting to hepatoma cells in vitro.

Aim: To increase exogenous gene expression level by modulating molecular conformations of targeting gene drugs.

Methods: The full length cDNAs of both P(40) and P(35) subunits of human interleukin 12 were amplified through polymerase chain reaction (PCR) and cloned into eukaryotic expressing vectors pcDNA3.1(+/-) to construct plasmids of P(+)/IL-12, P(+)/P(40) and P(-)/P(35).

[Distribution and metabodynamics of hepatocyte targeting vector molecules in vivo].

Objective: To testify whether asiaorosomucoid (ASOR), the ligamental molecule of specific hepatocyte receptor prepared in our laboratory, can be used as a delivery drug vector for targeting hepatocyte.

Methods: ASOR molecules marked with isotope 125I were injected into Strague-Dawley rats, and the distribution of ASOR on both organ and cellular levels was detected.

Results: ASOR could only combine with hepatocyte and distribute in the liver.