Emotional Variance Analysis: A new sentiment analysis feature set for Artificial Intelligence and Machine Learning applications.

Sentiment Analysis (SA) is a category of data mining techniques that extract latent representations of affective states within textual corpuses. This has wide ranging applications from online reviews to capturing mental states. In this paper, we present a novel SA feature set; Emotional Variance Analysis (EVA), which captures patterns of emotional instability.

Autoinducer-2-mediated quorum sensing partially regulates the toxic shock response of anaerobic digestion.

This study discovered a strong correlation between the autoinducer-2 (AI-2)-mediated quorum sensing (QS) with the performance of a submerged anaerobic membrane bioreactor during its recovery from a pentachlorophenol (PCP) shock: a decrease in AI-2 levels coincided with a reduction in volatile fatty acid concentrations, and corresponded significantly to a decrease in the relative abundance of Firmicutes, and to an increase in the relative abundance of Bacteroidetes and Synergistetes. Further batch experiments with the addition of an AI-2-regulating Escherichia coli mutant culture showed that a reduction in AI-2 levels resulted in the highest biogas production rate during a PCP shock. In contrast, an increase in AI-2 levels via addition of the E.

AI Paradigms for Teaching Biotechnology.

Artificial intelligence (AI) is profoundly changing biotechnological innovation. Beyond direct application, it is also a useful tool for adaptive learning and forging new conceptual connections within the vast network of knowledge for the advancement of biotechnology. Here, we discuss a new paradigm for biotechnology education that involves coevolution with AI.

Controlling a toxic shock of pentachlorophenol (PCP) to anaerobic digestion using activated carbon addition.

Several powdered and granular activated carbons (PACs and GACs) were tested for adsorption of pentachlorophenol (PCP) in bench-scale anaerobic digestion reactors to control the toxicity of PCP to acetoclastic methanogenesis. Results showed that the adsorption capacities of PAC were reduced by 21-54%, depending on the PAC addition time, in the presence of the methanogenic sludge compared to the controls without sludge. As a preventive measure, PAC at a low dose of 20% (mass ratio to the VSS) added 24 h prior to, or simultaneously with, the addition of PCP could completely eliminate the toxic effects of PCP.

Toxicity measurement in biological wastewater treatment processes: a review.

Biological wastewater treatment processes (WWTPs), by nature of their reliance on biological entities to degrade organics and sometimes remove nutrients, are vulnerable to toxicants present in their influent. Various toxicity measurement methods have been adopted for biological WWTPs, but most are performed off-line, and cannot be adapted to on-line monitoring tools to provide an early warning for WWTP operators. However, the past decade has seen a rapid expansion in the research and development of biosensors that can be used for toxicity assessment of aquatic environments.

A high-throughput microfluidic biochip to quantify bacterial adhesion to single host cells by real-time PCR assay.

A high-throughput microfluidic poly-(dimethylsiloxane) biochip was developed to quantify bacterial adhesion to single host cells by real-time PCR assay. The biochip is simply structured with a two-dimensional array of 900 micro-wells, one inlet, and one outlet micro-channels. Isolation of single infected host cells into the individual micro-wells of the biochip was achieved by one-step vacuum-driven microfluidics.

Facile fabrication of Ag/C-TiO nanoparticles with enhanced visible light photocatalytic activity for disinfection of Escherichia coli and Enterococcus faecalis.

This work aimed to investigate the promotive effect of Ag modification on C-TiO for visible light photocatalytic disinfection of microorganisms. Ag/C-TiO was prepared by a facile one-step synthesis approach through thermal oxidation of AgNO-impregnated TiC precursor. The self-doped carbon exists as both interstitial and substitutional carbon to collectively enhance the visible light photocatalytic absorption.

Dual fluorescence system for flow cytometric analysis of Escherichia coli transcriptional response in multi-species context.

When studying interspecies interactions in a bacterial consortium, it may be desirable to analyze one species' transcriptional response as influenced by the other species. We developed a dual fluorescence system of Escherichia coli for Fluorescence-Activated Cell Sorter (FACS)-based analysis for such a purpose. First, we generated E.

Temperature-regulated formation of mycelial mat-like biofilms by Legionella pneumophila.

Fifty strains representing 38 species of the genus Legionella were examined for biofilm formation on glass, polystyrene, and polypropylene surfaces in static cultures at 25 degrees C, 37 degrees C, and 42 degrees C. Strains of Legionella pneumophila, the most common causative agent of Legionnaires' disease, were found to have the highest ability to form biofilms among the test strains. The quantity, rate of formation, and adherence stability of L.

The role of the alarmone (p)ppGpp in sigma N competition for core RNA polymerase.

Some promoters, including the DmpR-controlled sigma(N)-dependent Po promoter, are effectively rendered silent in cells lacking the nutritional alarmone (p)ppGpp. Here we demonstrate that four mutations within the housekeeping sigma(D)-factor can restore sigma(N)-dependent Po transcription in the absence of (p)ppGpp. Using both in vitro and in vivo transcription competition assays, we show that all the four sigma(D) mutant proteins are defective in their ability to compete with sigma(N) for available core RNA polymerase and that the magnitude of the defect reflects the hierarchy of restoration of transcription from Po in (p)ppGpp-deficient cells.

Integration of global regulation of two aromatic-responsive sigma(54)-dependent systems: a common phenotype by different mechanisms.

Pseudomonas-derived regulators DmpR and XylR are structurally and mechanistically related sigma(54)-dependent activators that control transcription of genes involved in catabolism of aromatic compounds. The binding of distinct sets of aromatic effectors to these regulatory proteins results in release of a repressive interdomain interaction and consequently allows the activators to promote transcription from their cognate target promoters. The DmpR-controlled Po promoter region and the XylR-controlled Pu promoter region are also similar, although homology is limited to three discrete DNA signatures for binding sigma(54) RNA polymerase, the integration host factor, and the regulator.