Effect of bioactive Rosa roxburghii Tratt fruit polysaccharide on the structure and emulsifying property of lactoferrin protein.

Lactoferrin protein (LF) is a natural protein with certain emulsifying ability, but is sensitive to be affected by environmental factors and has poor oxidative stability to be used as emulsifier. In this study, the emulsifying ability of LF was significantly improved after conjugation with Rosa roxburghii Tratt fruit polysaccharides (RTFP), and the emulsion stability mechanism of LF-RTFP conjugates (L-R) were elucidated through the utilization of CLSM (confocal laser scanning microscopy), interfacial tension, apparent viscosity, and protein adsorption rate. The emulsion stabilized by L-R showed the smaller particle size (17.

Ultrasonic collaborative pulse extraction of sugarcane polyphenol with good antiaging and α-glucosidase inhibitory activity.

Sugarcane, as one important and heavily planted industrial crop, is meaningful to develop its byproducts. In this paper, the ultrasonic collaborative pulse was beneficial for the yield improvement and good bioactivity protection. The sugarcane polyphenol extract (SPE) yield reached 2.

Shape-Dependent Locomotion of DNA-Linked Magnetic Nanoparticle Films.

The shape-dependent aero- and hydro-dynamics found in nature have been adopted in a wide range of areas spanning from daily transportation to forefront biomedical research. Here, we report DNA-linked nanoparticle films exhibiting shape-dependent magnetic locomotion, controlled by DNA sequences. Fabricated through a DNA-directed layer-by-layer assembly of iron oxide and gold nanoparticles, the multifunctional films exhibit rotational and translational motions under magnetic fields, along with reversible shape morphing via DNA strand exchange reactions.

Microfracture Versus a Porcine-Derived Collagen-Augmented Chondrogenesis Technique for Treating Knee Cartilage Defects: Results at Midterm Follow-up.

Background: Damaged cartilage can be treated using the creation of microfractures (MFxs) or the porcine-derived collagen-augmented chondrogenesis technique (C-ACT).

Purpose: To provide the midterm results of a multicenter randomized controlled trial comparing MFx and C-ACT for knee cartilage defects.

Study Design: Randomized controlled trial; Level of evidence, 1.

The 3 I's of immunity and aging: immunosenescence, inflammaging, and immune resilience.

As we age, our immune system's ability to effectively respond to pathogens declines, a phenomenon known as immunosenescence. This age-related deterioration affects both innate and adaptive immunity, compromising immune function and leading to chronic inflammation that accelerates aging. Immunosenescence is characterized by alterations in immune cell populations and impaired functionality, resulting in increased susceptibility to infections, diminished vaccine efficacy, and higher prevalence of age-related diseases.

Development of a root-zone temperature control system using air-source heat pump and its impact on the growth and yield of paprika.

By developing and implementing a local temperature control system, such as a root zone, with a high energy efficiency heat source, we can ensure both yield and energy efficiency against extreme temperatures. This system, designed with practicality in mind, has a remarkably positive impact on paprika plants' growth and yield in greenhouse cultivation. In the summer season, paprika plants were grown with no cooling, nutrient solution cooling (NSC) and the combination of NSC and substrate surround cooling (SSC) (NSC + SSC).

Contrastive Speaker Representation Learning with Hard Negative Sampling for Speaker Recognition.

Speaker recognition is a technology that identifies the speaker in an input utterance by extracting speaker-distinguishable features from the speech signal. Speaker recognition is used for system security and authentication; therefore, it is crucial to extract unique features of the speaker to achieve high recognition rates. Representative methods for extracting these features include a classification approach, or utilizing contrastive learning to learn the speaker relationship between representations and then using embeddings extracted from a specific layer of the model.

Evaluation of Surgical Site Infection Rates in Traumatic Surgical Fixation and Arthroplasty Performed in Laminar Flow Versus Non-laminar Flow Theatres During the COVID Pandemic.

Introduction Laminar flow (LF) in theatres has become the standard of care in orthopaedic implant surgery. Most of the evidence for laminar flow use is based on arthroplasty surgery, with early studies showing a significant reduction in infections. We conducted a retrospective comparative study to assess surgical site infection (SSI) rates in consecutive patients undergoing surgery for trauma in LF and non-laminar flow (NLF) theatres.

Speckle reduced holographic display system with a jointly optimized rotating phase mask.

Speckle noise degrades image quality in systems with coherent light sources, which must be overcome in holographic displays. In this Letter, we introduce a holographic display system with a rotating phase mask for speckle noise reduction. The rotating phase mask works in a similar way as a conventional rotating diffuser, but its pattern is jointly optimized with the spatial light modulator to maintain the contrast of the reconstructed image.

Scenedesmus sp. as a phycoremediation agent for heavy metal removal from landfill leachate in a comparative study: batch, continuous, and membrane bioreactor (MBR).

Improper disposal of municipal solid waste led to the release of heavy metals into the environment through leachate accumulation, causing a range of health and environmental problems. Phycoremediation, using microalgae to remove heavy metals from contaminated water, was investigated as a promising alternative to traditional remediation methods. This study explored the potential of Scenedesmus sp.

Loss of the long form of Plod2 phenocopies contractures of Bruck syndrome-osteogenesis imperfecta.

Bruck syndrome is an autosomal recessive form of osteogenesis imperfecta caused by biallelic variants in PLOD2 or FKBP10 and is characterized by joint contractures, bone fragility, short stature, and scoliosis. PLOD2 encodes LH2, which hydroxylates type I collagen telopeptide lysines, a critical step for collagen crosslinking. The Plod2 global knockout mouse model is limited by early embryonic lethality, and thus, the role of PLOD2 in skeletogenesis is not well understood.

The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect .

Blackberry polysaccharides with certain molecular weight distribution have good bioactivity. In this research, type 2 diabetes mice were used to investigate the hypoglycemic effect of blackberry polysaccharides with three different molecular weights, BBP (603.59 kDa), BBP-8 (408.

Lysine Methyltransferase 2D Regulates Immune Response and Metastasis in Head and Neck Cancer.

Background/aim: The most frequently altered epigenetic modifier in head and neck squamous carcinoma (HNSC) is the histone methyltransferase KMT2D. KMT2D catalyzes methylation of histone H3K4 resulting in open chromatin and the activation of target genes. Tumor-associated macrophages (TAMs) promote cancer growth by causing T lymphocyte exhaustion.

Profiling protein-protein interactions to predict the efficacy of B-cell-lymphoma-2-homology-3 mimetics for acute myeloid leukaemia.

B-cell-lymphoma-2 (BCL2) homology-3 (BH3) mimetics are inhibitors of protein-protein interactions (PPIs) that saturate anti-apoptotic proteins in the BCL2 family to induce apoptosis in cancer cells. Despite the success of the BH3-mimetic ABT-199 for the treatment of haematological malignancies, only a fraction of patients respond to the drug and most patients eventually develop resistance to it. Here we show that the efficacy of ABT-199 can be predicted by profiling the rewired status of the PPI network of the BCL2 family via single-molecule pull-down and co-immunoprecipitation to quantify more than 20 types of PPI from a total of only 1.

Dynamics of high-speed electrical tree growth in electron-irradiated polymethyl methacrylate.

Dielectric materials are foundational to our modern-day communications, defense, and commerce needs. Although dielectric breakdown is a primary cause of failure of these systems, we do not fully understand this process. We analyzed the dielectric breakdown channel propagation dynamics of two distinct types of electrical trees.

Analysis of clinical and pathological characteristics of retroperitoneal paraganglioma and associated prognostic factors.

Background And Objectives: The aim of this study is to explore the long-term prognostic risk factors associated with patients diagnosed with retroperitoneal paraganglioma (RPGL) and examine their clinical and pathological characteristics.

Methods: Expressions of biomarkers were identified using immunohistochemistry (IHC) and case databases were retrospectively searched. Survival analysis was performed using Kaplan-Meier and Cox risk regression to identify the factors that influence the postoperative progression-free survival of patients with RPGL.

Construction of blackberry polysaccharide nano-selenium particles: Structure features and regulation effects of glucose/lipid metabolism in HepG2 cells.

In this study, blackberry polysaccharide-selenium nanoparticles (BBP-24-3Se) were first prepared via NaSeO/Vc redox reaction, followed by coating with red blood cell membrane (RBC) to form core-shell structure polysaccharide-selenium nanoparticles (RBC@BBP-24-3Se). The particle size of BBP-24-3Se (167.1 nm) was increased to 239.

Profiling of BCLxL Protein Complexes in Non-Small Cell Lung Cancer Cells via Multiplexed Single-Molecule Pull-Down and Co-Immunoprecipitation.

We introduce multiplexed single-molecule pull-down and co-immunoprecipitation, named m-SMPC, an analysis tool for profiling multiple protein complexes within a single reaction chamber using single-molecule fluorescence imaging. We employed site-selective conjugation of biotin and fluorescent dye directly onto the monoclonal antibodies, which completed an independent sandwich immunoassay without the issue of host cross-reactivity. We applied this technique to profile endogenous B-cell lymphoma extra-large (BCLxL) complexes in non-small cell lung cancer (NSCLC) cells.

Bioelectrochemical reactor to manage anthropogenic sulfate pollution for freshwater ecosystems: Mathematical modeling and experimental validation.

Anthropogenic sulfate loading into otherwise low-sulfate freshwater systems can cause significant ecological consequences as a biogeochemical stressor. To address this challenge, in situ bioremediation technologies have been developed to leverage naturally occurring microorganisms that transform sulfate into sulfide rather than implementing resource-intensive physio-chemical processes. However, bioremediation technologies often require the supply of electron donors to facilitate biological sulfate reduction.

Effect of accelerated aging on shelf-stability, product loss, sensory and biochemical characteristics in 2 lower quality beef cuts.

The aim of this study was to determine the impact of accelerated aging (AA) on shelf stability, product loss, sensory and biochemical characteristics of 2 lower quality beef cuts. Triceps brachii (TB) and semimembranosus (SM) were collected and fabricated from 10 USDA Choice beef carcasses and assigned to 1 of 6 treatments: 3 d cooler aged (control), 21 d cooler aged, AA 49 °C for 2 h, AA 49 °C for 3 h, AA 54 °C for 2 h, and AA 54 °C for 3 h. The results showed that AA can decrease APC counts on steak surface and in purge and redness, but increase lightness and product loss of the steaks (P < 0.

Ultra-fast genetically encoded sensor for precise real-time monitoring of physiological and pathophysiological peroxide dynamics.

Hydrogen Peroxide (HO) is a central oxidant in redox biology due to its pleiotropic role in physiology and pathology. However, real-time monitoring of HO in living cells and tissues remains a challenge. We address this gap with the development of an optogenetic hydRogen perOxide Sensor (oROS), leveraging the bacterial peroxide binding domain OxyR.

Bone health screening practices with dual-energy X-ray absorptiometry and prediction of abnormal results in pediatric inflammatory bowel disease.

Objectives: Pediatric patients diagnosed with inflammatory bowel disease (IBD) are at risk of suboptimal peak bone mass attainment. This study aimed to understand rates of bone health screening adherence, describe factors associated with dual-energy X-ray absorptiometry (DXA) acquisition, and identify factors associated with abnormal DXA.

Methods: We performed a retrospective cohort study of pediatric IBD patients over a 10-year time frame.

Structure-guided engineering of a fast genetically encoded sensor for real-time HO monitoring.

Hydrogen Peroxide (HO) is a central oxidant in redox biology due to its pleiotropic role in physiology and pathology. However, real-time monitoring of HO in living cells and tissues remains a challenge. We address this gap with the development of an optogenetic hydRogen perOxide Sensor (oROS), leveraging the bacterial peroxide binding domain OxyR.