[Treatment of tracheoesophageal fistula and consequences of severe spinal injury].

Modern approaches to intensive care and anesthesiology make it possible to save patients with various severe traumas. Prolonged mechanical ventilation allows stabilizing the patient's status, but can result severe complications including tracheoesophageal fistula. Our patient received severe combined injury after road accident, i.

[The flagship of the national naval medical science (on the 80th anniversary of establishment of the 1st Central Research Institute of the Defense Ministry of Russian Federation)].

The article is devoted to the 80th anniversary of the formation of the naval medical science subunit, which is the part of the 1st Central Research Institute of the Defense Ministry of Russian Federation. In the 30th years of XX century, a group of naval doctors formulated the main directions of preventive naval medicine. For eight decades, several generations of medical scientists have developed and ensured implementation of regulatory requirements for habitability and ergonomics of Navy ships.

[Taxonomic structure of Orthomyxoviridae: current views and immediate prospects].

Analysis of taxonomic structure of Orthomyxoviridae was undertaken in view of its anticipated evolution. Four concepts of circulation of influenza A viruses in the biosphere are discussed, viz. anthrponose, zooanthroponose, metastrongilose, and protozoan.

[Relationship of nuclear import of influenza virus nucleoprotein polymers to their conformational status].

It has been earlier shown that in the cells infected with influenza virus, the molecules of nucleoprotein (NP) are polymers that differ in their conformational maturity and stability. The present investigation has studied the ability of different conformational forms of NP polymers to migrate into the nucleus. Conformationally mature compact NP oligomers are shown to predominantly import into the nucleus.

[Native and renatured oligomer-dependent epitopes of intracellular influenza virus nucleocapsid protein].

Intracellular NP oligomers have been shown to react with some anti-NP monoclonal antibodies (mAbs) in radio-immnoprecipitation, immunoblotting, and dot immunoassay. Soluble NP monomers obtained after thermal dissociation of NP oligomers are not recognized by mAbs unlike the NP monomers whose concentration increased by about 100-fold due to transfer to the nitrocellulose membrane after polyacrylamide gel electrophoresis. The findings demonstrated that in the intact NP oligomers there were epitopes determined by their quaternary structure.

An antigenic epitope of influenza virus nucleoprotein (NP) associated with polymeric forms of NP.

Intracellular influenza virus nucleoprotein (NP) is characterized by a high efficiency of homo-polymers formation, however their antigenic structure is still incompletely known. Herein, we report that RNase-resistant intracellular NP homo-polymers have a highly ordered conformational antigenic epitope, which depends on inter-subunit interactions of monomeric NPs. Our studies have shown that in radioimmunoprecipitation (RIPA) intracellular NP polymers bind mAb N5D3 and RNase does not prevent their mAb binding.

[Complex environmental and virological monitoring in the Primorye Territory in 2003-2006].

The paper presents the results of monitoring of viruses of Western Nile (WN), Japanese encephalitis (JE), tick-borne encephalitis (TBE), Geta, Influenza A, as well as avian paramicroviruses type I (virus of Newcastle disease (ND)) and type 6 (APMV-6) in the Primorye Territory in 2003-2006. Totally throughout the period, specific antibodies to the viruses were detected by neutralization test in wild birds (7.3%, WN; 8.

[Two types of NP-NP associations in influenza virus-infected cells].

Two types of NP-NP associations are shown to form in the influenza virus-infected cells. Early NP synthesis gives rise to NP associations stabilized by relatively weak bonds. These structures are designed as NP multimers.

Non-compact nucleocapsid protein multimers in influenza-virus-infected cells.

We have previously shown that protease-resistant and highly immunoreactive compact NP oligomers, dissociating at +80 degrees C and possessing properties of folded proteins, are post-translationally formed in influenza-virus-infected cells. In this study we demonstrate that, in addition to compact NP oligomers, incompletely folded NP multimers are detected intracellularly by SDS/PAGE carried out under weak dissociating conditions. In cells infected with avian, human A(H2N2), and human A(H3N2) viruses, NP multimers are detected in the stacking gel of SDS/PAGE as retarded and loose structures dissociating at +50 degrees C.

[Newcastle disease virus in the populations of wild birds in the south of the Primorye Territory in the period of autumn migrations in 2001-2004].

The paper presents the results of molecular virological monitoring of Newcastle disease virus (NDV) by reverse-polymerase chain reaction (followed by sequence of F-gene fragment 374 p.n.) and chick embryo isolation of samples from the avian cloacal swabs collected in the south of the Primorye Territory in September-October 2001-2004.

Effects of chronic neuroticization on the monoaminergic systems of different structures in the brains of rats with different typological characteristics.

The levels of monoamines and their metabolites were studied by HPLC with electrochemical detection in homogenates of hypothalamus, hippocampus, prefrontal cortex, and amygdala in intact and neuroticized Wistar rats with different types of behavior in the open field and forced swimming tests. Intact rats with intermediate levels of activity and depressivity had higher serotonin concentrations in the hypothalamus and lower noradrenaline and hydroxyindoleacetic acid levels in the hippocampus than rats characterized by low activity and high depressivity. In neuroticization, the levels of study monoamines and their metabolites decreased in all the brain structures investigated with the exceptions of an increase in the dopamine concentration in the hippocampus and the dihydroxyphenylacetic acid concentration in the prefrontal cortex.

[The influence of chronic neurotization on the monoaminergic systems of various brain structures in rats with various typological characteristics].

Typological behavioral features of Wistar rats were tested in the open field and in Porsolt test. Rats were assigned to groups with high (HAct), medium (MAct), and low (LAct) behavioral activities. The same rats were assigned to high (HDep), medium (MDep) and low depressive (LDep) groups.

[Glial tumors of the brain: classification, present-day aspects of immunopathogenesis and immunogenetic diagnostics].

Glial tumors of the brain present a complicated problem in neurosurgery due to the peculiarities of their growth biology, their prevalence, infiltrative growth and diagnostic difficulties. The complexity of treatment, which includes surgical removal of a tumor, radiotherapy, chemotherapy, and immune correction, makes the problem even more topical. Modem classification of glial neoplasms is of no less importance, because treatment depends on the histologic structure of a tumor.

[Temporary acquisition of intra-chain disulphide bonds by nucleocapsid protein of influenza virus].

The in vitro reducing agents were shown to promote the NP-NP association and to stabilize the NP oligomers, which dissociate when heated in non-reducing buffer. This confirms that non-covalent linkages in electrophoresis stabilize the influenza virus NP oligomers. The mobility of pulse-labeled and chased NPs in PAGE as well as their sensitivity to protease were investigated.

Stability of intracellular influenza virus nucleocapsid protein oligomers.

Stability of A/Duck/Ukrainae/63 (H3N8) influenza virus intracellular NP oligomers was studied using reducing agents, denaturants, detergents, salts, various pH and a range of temperatures. The results obtained indicate that influenza virus NP oligomers are noncovalently stabilized, and NP subunits are not linked by disulfide bonds. NP oligomers are thermostable and SDS resistant.

Transient disulfide bonds formation in conformational maturation of influenza virus nucleocapsid protein (NP).

It has been previously shown that influenza virus nucleocapsid protein (NP) forms homooligomers in vivo. Our analyses revealed that the reducing agent dithiothreitol (DTT) introduced in pulse labeling period prevented further formation of native NP-oligomers. The shortly pulse-labeled non-reduced newly synthesized NP possessed a relatively faster mobility in non-reducing PAGE and a higher resistance to protease than the reduced one.

[Differences in oligomerization of nucleocapsid protein of epidemic human influenza A(H1N1), A(H1N2) and B viruses].

A comparative analysis of involving the nucleocapsid protein (NP) into shaping-up of SDS-resistant oligomers was carried out presently in circulating epidemic strains of human influenza, viruses A and B. The study results of viral isolates obtained from clinical samples and recent standard strains revealed that the involvement of NP in the SDS-resistant oligomers, which are different in various subtypes of influenza A viruses. According to this sign, the human viruses A(9H3N2) are close to the avian ones, in which, as proved by us previously, virtually the entire NP transforms itself into the oligomers resistant to SDS.

[The formation of the influenza virus aggregates, enriched with hemagglutinin].

The formation of electrostatic aggregates was studied by analysis of two types of virus-containing liquids: initial warm liquid collected at temperature 37 degrees and the same liquid stored over the night at temperature 4 degrees C. The formation of virus aggregates was revealed at 4 degrees C. The aggregates formed at temperature 4 degrees C had a relatively high HA/NP ratio in comparison with unassociated virus analyzed at 37 degrees.

Avian and human influenza a virus strains possess different intracellular nucleoprotein oligomerization efficiency.

We have previously shown (Prokudina-Kantorovich EN and Semenova NP, Virology 223, 51-56, 1996) that the nucleoprotein (NP) of influenza A virus forms in infected cells oligomers which in the presence of SDS and 2-mercaptoethanol (ME) as reducing agent are stable at room temperature (RT) and dissociate at 100 degrees C. Here we report that the efficiency of intracellular NP oligomerization depends on the host origin of influenza A virus strain. Thus, in the cells infected with avian influenza A virus strains the viral NP was almost completely oligomerized and only traces of monomeric NP were detected by polyacrylamide gel electrophoresis (PAGE) in unboiled samples.

Extracellular truncated influenza virus nucleoprotein.

In the culture medium of MDCK cells infected with influenza A/Duck/Ukraine/1/63(H3N8) virus two kinds of virus nucleoprotein (NP) are detected: full-length 56 kDa NP and truncated 53 kDa NP. However, in infected cells 53 kDa NP may be detected only at short pulse and after 10 min chase it becomes nondetectable. The extracellular truncated 53 kDa NP is detected in free RNP, and not in the virions.

[Extracellular immunoreactive nucleoprotein of Influenza virus not related to the virion].

Extracellular immunoreactive virus NP is accumulated in virus-containing fluid in the course of A/Duck/Ukraine/1/63(H3N8) influenza virus infection. The major part of this extracellular NP is included in viral RNP and characterized by relatively low molecular weight: 53 kD vs. 56 kD of virion NP.

[Dependence of oligomerization of influenza virus nucleoprotein on the species affiliation of the virus].

Comparison of human and avian influenza virus nucleoprotein (NP) oligomerization showed that the efficiency of NP oligomerization is different in influenza viruses of different origin. NP oligomerization is virtually complete in avian influenza viruses, while in human influenza viruses only part of monomeric NP is oligomerized. The authors discuss the utilization of NP oligomerization efficiency as a sign for identification of the origin of influenza virus.

[The development of the lymphatic vessel wall in the early period of swine postnatal ontogeny].

Using histological methods and transmission electron microscopy differences in architecture of lymphatic vessel wall between newborn and 2 months old pigs were established. The number of free ribosomes and polysomes is smaller and that of pinocytotic vesicles is higher in 2 months pigs. Contacts between endotheliocytes are presented in three types.