Optimization of fermentation conditions for whole cell catalytic synthesis of D-allulose by engineering Escherichia coli.

D-allulose/D-psicose is a significant rare sugar with broad applications in the pharmaceutical, food, and other industries. In this study, we cloned the D-allulose 3-epimerase (DPEase) gene from Arthrobacter globiformis M30, using pET22b as the vector. The recombinant E.

Enhancing the erythritol production of Yarrowia lipolytica by high-throughput screening based on highly sensitive artificial sensor and anchor protein cwp2.

Unlabelled: Yarrowia lipolytica is widely used for the industrial production of the natural sweetener erythritol. Despite improvements in fermentation process control and metabolic pathway regulation, bottlenecks still exist in terms of yield and screening technology. Therefore, we constructed an artificial sensor system for effective erythritol detection, established a single-cell droplet-based high-throughput screening system based on fluorescence-activated cell sorting, and obtained Y.

Improvement of optimum pH and specific activity of pectate lyase from RN.1 using loop replacement.

Alkaline pectate lyase plays an important role in papermaking, biological refining and wastewater treatment, but its industrial applications are largely limited owing to its low activity and poor alkali resistance. The alkaline pectate lyase BspPel from RN.1 was heterologously expressed in BL21 (DE3) and its activity and alkali resistance were improved by loop replacement.

Study on the construction technology of β-alanine synthesizing based on cellulosome assembly.

β-Alanine is the only β-amino acid in nature; it is widely used in food additives, medicines, health products, and surfactants. To avoid pollution caused by traditional production methods, the synthesis of β-alanine has been gradually replaced by microbial fermentation and enzyme catalysis, which is a green, mild, and high-yield biosynthesis method. In this study, we constructed an recombinant strain for efficient β-alanine production using glucose as the raw material.

Design and application of artificial rare L-lysine codons in .

L-lysine is widely used in the feed, food, and pharmaceutical industries, and screening for high L-lysine-producing strains has become a key goal for the industry. We constructed the rare L-lysine codon AAA by corresponding tRNA promoter replacement in . Additionally, a screening marker related to the intracellular L-lysine content was constructed by converting all L-lysine codons of enhanced green fluorescent protein (EGFP) into the artificial rare codon AAA.

Increasing L-lysine production in Corynebacterium glutamicum by engineering amino acid transporters.

Corynebacterium glutamicum has a long and successful history in the biotechnological production of L-lysine. Besides the adjustment of metabolic pathways, intracellular and extracellular transport systems are critical for the cellular metabolism of L-lysine or its by-products. Here, three amino acid transmembrane transporters, namely, GluE, BrnE/BrnF, and LysP, which are widely present in C.