[Treatment of nasal malignant tumor with electron beam irradiation after repair of nasolabial flap].

Objective: To explore the appropriate reconstructive methods and curative effect after nasal skin malignant tumor excision.

Method: Nasolabial flap was selected to cover the nasal defects in 15 cases which nasal tumors were removed. The secondary defect of donor site was directly sutured.

[Experiment on autologous free granular fat transplantation with rhVEGF gene in rats].

Objective: To investigate the effects of rhVEGF on autologous free granular fat grafts in rats.

Methods: Forty-eight Sprague-Dawley rats were randomly divided into three groups, sixteen of each. After the autologous free granular fat transplantation, all groups were treated with the plasmid DNA containing cDNA encoding rhVEGF, the blank plasmid DNA and normal saline respectively as the experimental group, the negative group and the saline group.

[Immunological effects of polyacrylamide hydrogel injection in human body].

Objective: To observe the immunologic changes in patients who have complications after polyacrylamide hydrogel (HPG) injection.

Methods: To compare the patients in the HPG injection group and the control group, the expressions of immunoglobulin IgG, IgM, IgA and complement C3, C4 of serum were measured by immunoturbidimetry.

Results: The expressions of IgG and C3 in the injection group were lower than the control group with extremely significant difference (P < 0.

[The expression and interaction of cyclin D1 and p16 in fibroblasts of pathologic scars].

Objective: To investigate the expression of cyclin D1, p16, AR and ER in fibroblasts of scars for further understanding the interaction of these factors and the roles that they play in scar development.

Methods: Thirty samples of mature scar, hypertrophic scar and keloid were detected with immunohistochemical method (SP technique) and compared with normal skin.

Results: There were on positive results in normal skin and mature scars.

[The immediate local skin flaps transference for the treatment of acute auricle trauma defect].

Objective: To investigate the advantage and postoperation notice of immediate local skin flaps transference for the treatment of acute auricle trauma defect.

Method: A retrospective study was carried out to judge the immediate local skin flaps transference for sixty-three patients who underwent ear injury.

Result: All the flaps survived postoperation.

[Vascular endothelial growth factor gene transfection and survival of the random skin flap in rats].

Objective: To investigate the effects of vascular endothelial growth factor (VEGF) gene transfection on survival of the random skin flap in rats.

Method: Thirty SD rats were randomized equally into 3 groups: pcDNA3-VEGF165, pcDNA3 and control groups, with the former two groups transfected via liposome with pcDNA3-VEGF165 and pcDNA3 respectively 48 h before and during the operation. Ischemic random skin flaps ( 1 cmx7 cm) were constructed from the rats.

[An experimental study on the survival of the anterior abdominal skin flap after VEGF cDNA administration].

Objective: To investigate flap survival after transfection using gene encoding vascular endothelial growth factor (VEGF).

Methods: In 30 Sprague-Dawley rats, the anterior abdominal skin flap supplied by the epigastric vessels was created. The animals were divided into three groups, with ten of each.