Molecular cloning and functional characterization of UBC13 and MMS2 from Candida albicans.

To maintain genome stability, eukaryotes have evolved a powerful DNA damage response system called DNA-damage tolerance (DDT) to deal with replication-blocking lesions. In the budding yeast Saccharomyces cerevisiae, K63-linked polyubiquitination of proliferating cell nuclear antigen (PCNA) is mediated by a Ubc13-Mms2 heterodimer, leading to error-free DDT. Candida albicans is one of the most studied fungal pathogens and to date no data regarding K63-linked ubiquitination or error-free DDT has been available.

[Analysis of relevant risk factors to Henoch-Schönlein purpura in Tibetan children].

Objective: To analyze probable risk factors to Henoch-Schönlein purpura (HSP) in Tibetan children so as to bring evidences for correct identification of high-risk children in plateau areas.

Methods: 140 high-altitude Tibetan children with HSP admitted to Shannan People's Hospital of Tibet Autonomous Region from October 2015 to October 2018 were enrolled, and 140 high-altitude Tibetan healthy children and 140 plain area HSP children were selected as the control. Gender, age, family history, allergy, past history (rheumatic disease, autoimmune disease, asthma), clinical phenotype, biochemical markers (antibody positive rate, platelet count and hemoglobin), clinical efficacy and recurrence were retrospective analyzed.

Utilization of a Strongly Inducible Promoter to Control Gene Expression in .

Regulating target gene expression is a common method in yeast research. In , there are several widely used regulated expression systems, such as the and Tet-off systems. However, all current expression systems possess some intrinsic deficiencies.