Natamycin and Azithromycin Are Synergistic against Ocular Pathogenic Aspergillus flavus Species Complex and Fusarium solani Species Complex Isolates.

The interaction of natamycin-azithromycin combination against 60 ocular fungal isolates was tested The combination produced 100% synergistic interactions when natamycin was added to azithromycin 20, 40, and 50 μg/ml against species complex (AFSC) isolates and to azithromycin 50 μg/ml against species complex isolates. Addition of 50 μg/ml azithromycin enhanced natamycin's effect against AFSC isolates by reducing natamycin MIC from 64 to 0.031 μg/ml.

Rifampin Enhances the Activity of Amphotericin B against Fusarium solani Species Complex and Aspergillus flavus Species Complex Isolates from Keratitis Patients.

The activities of amphotericin B in combination with rifampin were assessed against 95 ocular fungal isolates. The interactions between amphotericin B and rifampin at 4, 8, 16, and 32 μg/ml were synergistic for 11.8%, 51.

Effects of contact lens solution disinfectants against filamentous fungi.

Purpose: To assess and compare the antifungal activity of polyhexamethylene biguanide (PHMB), thimerosal, cetylpyridinium chloride, and chlorhexidine, which are disinfectants used in multipurpose disinfectant solutions (MPDSs) against ocular pathogenic Fusarium solani and Aspergillus flavus isolates in vitro.

Methods: The in vitro activity of PHMB, thimerosal, cetylpyridinium chloride, and chlorhexidine was assessed against 40 isolates of ocular pathogenic fungi that included 24 F. solani and 16 A.

Analysis of 24-hour monitoring of intraocular pressure in 1055 eyes.

Purpose: To investigate the clinical significance of 24-hour monitoring of intraocular pressure (IOP).

Methods: A total of 571 cases (1055 eyes) undergoing 24-hour monitoring of IOP in the Second People's Hospital of Zhengzhou between June 2012 and March 2013 were retrospectively analyzed.

Results: Among all 1055 eyes, 298 had suspected glaucoma (28.

In vitro antifungal activity of silver nanoparticles against ocular pathogenic filamentous fungi.

Purpose: Fungal keratitis is emerging as a major cause of vision loss in a developing country such as China because of higher incidence and the unavailability of effective antifungals. It is urgent to explore broad-spectrum antifungals to effectively suppress ocular fungal pathogens, and to develop new antifungal eye drops to combat this vision-threatening infection. The aim of this study is to investigate the antifungal activity of silver nanoparticles (nano-Ag) in comparison with that of natamycin against ocular pathogenic filamentous fungi in vitro.

Antifungal effect of ophthalmic preservatives phenylmercuric nitrate and benzalkonium chloride on ocular pathogenic filamentous fungi.

In the present study, the antifungal effects of phenylmercuric nitrate and benzalkonium chloride versus those of natamycin and ketoconazole were assessed against 216 filamentous fungi isolates from cases of fungal keratitis. They included 112 Fusarium isolates, 94 Aspergillus isolates, and 10 Alternaria alternata isolates. The strains were tested by broth dilution antifungal susceptibility testing of filamentous fungi approved by the Clinical and Laboratory Standards Institute M38-A document.

In vitro activity of phenylmercuric acetate against ocular pathogenic fungi.

Objectives: To determine the antifungal activity of phenylmercuric acetate against ocular pathogenic fungi in vitro and develop new antifungal eye drops to combat keratomycosis.

Methods: The in vitro activity of phenylmercuric acetate was assessed against 261 isolates of ocular pathogenic fungi that included 136 Fusarium spp. isolates, 98 Aspergillus spp.

[Activity of butenafine against ocular pathogenic filamentous fungi in vitro].

Objective: To investigate antifungal activity of butenafine in comparison with that of natamycin, amphotericin B and fluconazole against ocular pathogenic filamentous fungi in vitro.

Methods: It was an experimental study. Susceptibility tests were performed against 260 isolates of ocular pathogenic filamentous fungi by broth dilution antifungal susceptibility test of filamentous fungi approved by the Clinical and Laboratory Standards Institute (CLSI) M38-A document.

[Comparison of the activities of silver nitrate with those of three antifungal agents against ocular pathogenic fungi in vitro].

Objective: To investigate antifungal activity of silver nitrate compared with fluconazole, ketoconazole and amphotericin B against ocular pathogenic fungi in vitro.

Methods: It was an experimental study. Susceptibility tests were performed against 260 isolates (15 genera and 29 species) of ocular pathogenic fungi by broth dilution antifungal susceptibility testing of filamentous fungi (M38-A) approved by National Committee for Clinical Laboratory Standards (NCCLS).

In vitro activity of thimerosal against ocular pathogenic fungi.

The in vitro activity of thimerosal versus those of amphotericin B and natamycin was assessed against 244 ocular fungal isolates. The activity of thimerosal against Fusarium spp., Aspergillus spp.

In vitro comparison of the efficacies of natamycin and silver nitrate against ocular fungi.

The in vitro activity of the silver nitrate was assessed in comparison with that of natamycin against 128 corneal Fusarium isolates and 90 corneal Aspergillus isolates. MIC(90)s of silver nitrate were 2 microg/ml for Fusarium spp. and 1 microg/ml for Aspergillus spp.