Molecular Mechanism of Gene Overexpression in Enhancing Monacolin K Production in .

species are capable of producing various active metabolites, including monacolin K (MK) and pigments. Studies have shown that the overexpression of the gene from the MK synthesis gene cluster in species can significantly increase MK production; however, the molecular mechanism has not yet been fully elucidated. Therefore, this study focused on the gene of to construct overexpression strains of the gene, resulting in high-yield MK production.

Identification and functional characterization of glycerol dehydrogenase reveal the role in kojic acid synthesis in Aspergillus oryzae.

Glycerol dehydrogenase has been identified and characterized functionally in many species. However, little is known about glycerol dehydrogenase genes and their functions in Aspergillus oryzae. Here, a total of 45 glycerol dehydrogenase genes in Aspergillus oryzae were identified and renamed from AoGld1 to AoGld45 according to their chromosome distribution.

The alpha-amylase MrAMY1 is better than MrAMY2 in rice starch degradation, which promotes pigments production in .

Previously, the alpha amylase-encoding gene from was heterologously expressed in CICC41233 to promote starch hydrolysis and increase the production of pigments. The target of this study is to screen the effective alpha-amylases from for starch fast degradation and investigated for pigments production. The 13 types of predicted alpha-amylases in the NRRL1597 genome were divided into four classes based on EC number and into five groups based on the glycoside hydrolase sub-family.

Identification and characterization of the ZRT, IRT-like protein (ZIP) family genes reveal their involvement in growth and kojic acid production in Aspergillus oryzae.

The ZRT, IRT-like protein (ZIP) family exists in many species and plays an important role in many biological processes, but little is known about ZIP genes in Aspergillus oryzae. Here, 10 ZIP genes in A. oryzae were identified and these were classified into four groups based on phylogenetic analysis.

An overview of the bioactivity of monacolin K / lovastatin.

Monacolin K (MK) is the principal active substance in Monascus-fermentation products (e.g. red yeast rice).

Highly efficient improvement of pigment production by accelerating starch hydrolysis in CICC41233.

To investigate the relationship between starch hydrolysis and pigments (MPs) production, the α-amylase gene () from was heterologously expressed in CICC41233, and we obtained a positive transformant named Amy9. In Amy9, the α-amylase activities were 6.65- and 4.

The acyl-CoA binding protein affects pigment production in CICC41233.

The present study verified whether acyl-coenzyme A (acyl-CoA)-binding protein (ACBP) affected the production of pigments (MPs) in CICC41233 (MrACBP). Phylogenetic analysis revealed that the cloned gene, which encoded the MrACBP protein, exhibited the closest match (99% confidence level) to the gene from . The MrACBP and maltose-binding protein (MBP) were simultaneously expressed in Rosetta DE3 in the form of a fusion protein.

Improvement in xylooligosaccharides production by knockout of the - gene in EU7-22.

The goal of this study was to enhance the production of xylooligosaccharides (XOs) and reduce the production of xylose. We investigated β-xylosidases, which were key enzymes in the hydrolysis of xylan into xylose, in EU7-22. The binary vector pUR5750G/:: was constructed to knock out the - gene (encoding β-xylosidases) in EU7-22 by homologous integration, producing the mutant strain Bxyl-1.

Enhancing Cellulase and Hemicellulase Production in Trichoderma orientalis EU7-22 via Knockout of the creA.

The role of the transcription factor creA-mediating carbon catabolite repression in Trichoderma orientalis EU7-22 was investigated for cellulase and hemicellulase production. The binary vector pUR5750G/creA::hph was constructed to knock out creA by homologous integration, generating the ΔcreA mutant Trichoderma orientalis CF1D. For strain CF1D, the filter paper activities (FPA), endoglucanase activities (CMC), cellobiohydrolase activity(CBH), β-glucosidase activity (BG), xylanase activity (XYN), and extracellular protein concentration were 1.

Deep sequencing analysis of transcriptomes in Aspergillus oryzae in response to salinity stress.

Characterization of the changes after various stimuli is crucial to comprehend the adaptation of cells to the changing condition. Aspergillus oryzae is widely used for the industrial production of soy sauce, which always encounter changes within a complex environment, such as salinity stress. However, the protective biochemical mechanisms of A.

Rapid analysis of mono-saccharides and oligo-saccharides in hydrolysates of lignocellulosic biomass by HPLC.

HPLC using pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) was used to analyse mono-saccharides and oligo-saccharides in hydrolysates of lignocellulosic biomass. PMP derivatives, including those of mannose, rhamnose, cellobiose, glucose, xylose and arabinose, were separated within 14 min with detection at 254 nm. The method was also suitable for xylo-oligosaccharides (XOS): PMP derivatives of xylohexaose, xylopentaose, xylotetraose, xylotriose and xylobiose were well separated under the same conditions.

Identification of a genomic region containing a novel promoter resistant to glucose repression and over-expression of β-glucosidase gene in Hypocrea orientalis EU7-22.

A high concentration of glucose in the medium could greatly inhibit the expression of cellulase in filamentous fungi. The aspartic protease from fungus Hypocrea orientalis EU7-22 could efficiently express under both induction condition and glucose repression condition. Based on the sequence of structure gene of aspartic protease, the upstream sequence harboring the putative promoter proA for driving the expression of aspartic protease was obtained by genome walking.

Cellulase production in a new mutant strain of Penicillium decumbens ML-017 by solid state fermentation with rice bran.

To produce cellulolytic enzyme efficiently, Penicillium decumbens strain L-06 was used to prepare mutants with ethyl methane sulfonate (EMS) and UV-irradiation. A mutant strain ML-017 is shown to have a higher cellulase activity than others. Box-Behnken's design (BBD) and response surface methodology (RSM) were adopted to optimize the conditions of cellulase (filter paper activity, FPA) production in strain ML-017 by solid-state fermentation (SSF) with rice bran as the substrate.

[Screening, identifying of cellulose-decomposing strain L-06 and its enzyme-producing conditions].

Cellulases are relatively costly enzymes that are sold in large volumes for use in different industrial applications, and a significant reduction in cost will be important for their commercial use in biorefineries. The production of cellulase is a major factor in the hydrolysis of cellulosic materials. Hence it is essential to make the process economically viable.