An Efficient System for Transposon Tagging in .

Transposon tagging is a powerful tool that has been widely applied in several species for insertional mutagenesis in plants. Several efforts have aimed to create transfer-DNA (T-DNA) insertional mutant populations in , a monocot plant used as a model system to study temperate cereals, but there has been a lack of research aimed at using transposon strategies. Here, we describe the application of a maize () () transposon tagging system in The :: cassette and element were constructed within the same T-DNA and transformed into plants.