Driving effects of spatiotemporal evolution of the water resources carrying capacity in the Yellow River Basin (Henan section).

The water resources carrying capacity (WRCC) is a crucial indicator for assessing social, economic and ecological sustainability supported by water resources. While there are abundant studies on the WRCC, there remains a need to thoroughly analyze the driving effects of WRCC evolution. In this study, an index-based WRCC evaluation system was constructed, and a comprehensive evaluation model was employed to assess the spatiotemporal evolution of the WRCC in the Yellow River Basin (YRB) (Henan section).

Relationship between immune cells and diabetic nephropathy: a Mendelian randomization study.

Objective: Although previous studies have suggested potential correlations between immunocytes and diabetic nephropathy (DN), the causal correlations between them remain unclarified. In this study, we employed Mendelian randomization (MR) to analyze the potential causative correlations between immune 731 cells and DN.

Methods: We used the Genome-Wide Association Studies (GWAS) database to aggregate signatures of immune cells and DN from European and East Asian populations.

Characterization and Function of Glycans on the Spike Proteins of SARS-CoV-2 Variants of Concern.

SARS-CoV-2 variants of concern (VOCs) pose a great challenge to viral prevention and treatment owing to spike (S) protein mutations, which enhance their infectivity and capacity for immune evasion. However, whether these S protein mutations affect glycosylation patterns and thereby influence infectivity and immunogenicity remains unclear. In this study, four VOC S proteins-S-Alpha, S-Beta, S-Delta, and S-Omicron-were expressed and purified.

Near-Infrared Luciferase Complementation Assay with Enhanced Bioluminescence for Studying Protein-Protein Interactions and Drug Evaluation Under Physiological Conditions.

Identification of protein-protein interactions (PPIs) that occur in various cellular processes helps to reveal their potential molecular mechanisms, and there is still an urgent need to develop the assays to explore PPIs in living subjects. Here, we reported a near-infrared split luciferase complementation assay (SLCA) with enhanced bioluminescence produced by cleaving a luciferase, Akaluc, for exploring and visualizing PPIs in living cells and live mice. Compared with the previously developed and widely used red SLCA based on split firefly luciferase (Fluc-SLCA), the signal intensities for PPI recognition in living cells and live mice of the Akaluc-SLCA increased by ∼3.

Synthesis of Dual-Emitting CdZnSe/Mn:ZnS Quantum Dots for Sensing the pH Change in Live Cells.

Intracellular pH is an important regulator of cell function, and its subtle changes may greatly affect cell activities and cause diseases. Reliable imaging of intracellular pH remains a huge challenge. Dual-emitting Mn-doped quantum dots (QDs) can be directly used as a ratiometric fluorescent probe without further modification, but they displayed low performance in terms of photoluminescence, stability, and intensity ratio regulation.

Aridity modifies the responses of plant stoichiometry to global warming and nitrogen deposition in semi-arid steppes.

Global warming and nitrogen (N) deposition are known to unbalance the stoichiometry of carbon (C), N, and phosphorus (P) in terrestrial plants, but it is unclear how water availability regulates their effects along a natural aridity gradient. Here, we conducted manipulative experiments to determine the effects of experimental warming (WT) and N addition (NT) on plant stoichiometry in desert, typical, and meadow steppes with decreasing aridity. WT elevated air temperatures by 1.

The smallest near-infrared fluorescence complementation system for imaging protein-protein and RNA-protein interactions.

Bimolecular fluorescence complementation (BiFC) and its derivative molecular biosensor systems provide effective tools for visualizing biomolecular interactions. The introduction of red and near-infrared fluorescence emission proteins has expanded the spectrum of signal generating modules, enabling BiFC for imaging. However, the large size of the signal module of BiFC can hinder the interaction between proteins under investigation.

The intraviral protein-protein interaction of SARS-CoV-2 reveals the key role of N protein in virus-like particle assembly.

Intraviral protein-protein interactions (PPIs) of SARS-CoV-2 in host cells may provide useful information for deep understanding of virology of SARS-CoV-2. In this study, 22 of 55 interactions of the structural and accessory proteins of SARS-CoV-2 were identified by biomolecular fluorescence complementation (BiFC) assay. The nucleocapsid (N) protein was found to have the most interactions among the structural and accessory proteins of SARS-CoV-2, and also specifically interacted with the putative packaging signal (PS) of SARS-CoV-2.

SARS-CoV-2 spike protein receptor-binding domain N-glycans facilitate viral internalization in respiratory epithelial cells.

N-glycosylation plays an important role in the pathogenesis of viral infections. However, the role of SARS-CoV-2 RBD N-glycosylation in viral entry remains elusive. In this study, we expressed and purified N331 and N343 N-glycosite mutants of SARS-CoV-2 RBD.

Pavement 3D Data Denoising Algorithm Based on Cell Meshing Ellipsoid Detection.

As a new measuring technique, laser 3D scanning technique has advantages of rapidity, safety, and accuracy. However, the measured result of laser scanning always contains some noise points due to the measuring principle and the scanning environment. These noise points will result in the precision loss during the 3D reconstruction.

A tandem near-infrared fluorescence complementation system with enhanced fluorescence for imaging protein-protein interactions in vivo.

Bimolecular fluorescence complementation (BiFC) is an effective tool for visualizing protein-protein interactions (PPIs). However, a BiFC system with long wavelength and high fluorescence intensity is yet to be developed for in vivo imaging. In this study, we constructed a tandem near-infrared BiFC (tBiFC) system by splitting a near-infrared phytochrome, IFP2.

Aridity stimulates responses of root production and turnover to warming but suppresses the responses to nitrogen addition in temperate grasslands of northern China.

Global warming and nitrogen (N) deposition are known to affect root dynamics in grasslands. However, previous studies were based only on a single ecosystem type, so it is unclear how warming and N addition affect root traits (root biomass, root-shoot ratio, root production and turnover) along the aridity gradient. In this study, we conducted an experiment to determine the effects of warming and N addition on root traits in desert, typical, and meadow grasslands in northern China, where the aridity gradually decreases from west to east across the region.

Nonlinear Optimization of Orthotropic Steel Deck System Based on Response Surface Methodology.

The steel bridge deck system, directly subjected to the vehicle load, is an important component to be considered in the optimization design of the bridges. Due to its complex structure, the design parameters are coupled with each other, and many fatigue details in the system result in time-consuming calculation during structure optimization. In view of this, a nonlinear optimization method based on the response surface methodology (RSM) is proposed in this study to simplify the design process and to reduce the amount of calculations during optimization.

An EDTA-resistant pyrazinamidase from non-pathogen Pseudonocardia carboxydivorans.

Objectives: To characterize a pyrazinamidase from non-pathogen Pseudonocardia carboxydivorans.

Results: A pyrazinamidase gene pncA encoding a 23-kDa protein PncA-Pse from P. carboxydivorans was over-expressed in Escherichia coli and characterized.

A meta-analysis of 1,119 manipulative experiments on terrestrial carbon-cycling responses to global change.

Direct quantification of terrestrial biosphere responses to global change is crucial for projections of future climate change in Earth system models. Here, we synthesized ecosystem carbon-cycling data from 1,119 experiments performed over the past four decades concerning changes in temperature, precipitation, CO and nitrogen across major terrestrial vegetation types of the world. Most experiments manipulated single rather than multiple global change drivers in temperate ecosystems of the USA, Europe and China.

Antagonistic effect of protein extracts from on pathogenic bacteria and fungi of the oral cavity.

An antibacterial substance from () is known to have an inhibitory effect on putative periodontal pathogens, but its inhibitory effect on pathogens of oral candidiasis is unknown. In this study, intracellular and exocrine proteins were extracted from . The antagonistic effect of the protein extracts on () and () was detected by a well-plate technique, and the effects of the protein extracts on biofilms formed by these bacteria were evaluated by confocal laser scanning microscopy.