[Study on interference therapy induced by epidermal growth factor receptor-antisense cDNA in signal transduction of laryngeal squamous cell carcinoma].

Objective: To investigate effect of interference therapy induced by epidermal growth factor receptor (EGFR)-antisense cDNA in signal transduction of Hep-2 laryngeal squamous cell carcinoma in vitro.

Methods: AdEasy Vector System was used to construct the recombinant adenovirus vector sense/antisense-pAdEasy-EGFR. The recombinant adenovirus vector introduced EGFR-sense/antisense cDNA fragment into HEK293 cell.

[Interference therapy of laryngeal squamous cell carcinoma by p16beta and EGFR-antisense cDNA in signal transduction].

Objective: To test the effect of p163 and EGFR-antisense cDNA in signal transduction on Hep-2 laryngeal squamous cell carcinoma in vitro.

Methods: The Hep-2 laryngeal squamous carcinoma cells were transfected by recombinant adenovirus AdEasy-EGFR-antisense and AdEasy-p16beta in vitro. The inhibition of the EGFR expression and cell growth and changes of cell cycle, DNA content, apoptosis and ultramicrostructure of the Hep-2 cells were examined by MTT, Western blotting analysis, Flow cytometry analysis, Immunohistochemistry, and transmission electron microscope respectively.

[Microsurgical anatomy of epiglottic artery and its clinical implications].

Objective: To study the microsurgical anatomy of human epiglottic blood vessel to provide exact and reliable data and morphological properties for further studies of laryngeal transplantation, reconstruction and other epiglottis-related diseases.

Methods: Thirty Chinese adult cadavers (27 males and 3 females) were examined for the present study. The cadavers were dissected under magnification along the anatomic planes from skin down to vertebral column.

[Specific inhibition of Raf-1 gene expression in HNE1 by RNA interference].

Objective: To silence the expression of Raf-1 gene in HNE1 cells using vector-based RNA interference (RNAi) technique.

Methods: The vector containing the human U6 promoter was used for targeted gene silencing when a dsDNA oligonucleotide encoding an appropriate shRNA was ligated into the vector, and 67nt oligonucleotide fragment was inserted into the downstream of the U6 promoter. Plasmids containing different Raf-1 target sequences [ (1) pshuttle-Raf-1-a( 225), (2) pshattle-Raf-1-b ( 358) and (3) pshuttle-Raf-1-c(474)], were transfected into HNE1 cells.

Aberrant branch of the superior laryngeal artery passing through the thyroid foramen.

This study examines the microsurgical anatomy of the aberrant superior laryngeal artery (ASLA) which passes through the thyroid foramen. It defines the location of the ASLA and its main branches. The aberrant superior thyroid artery was looked for in 20 randomly selected Chinese adult cadavers, and when found, was dissected and measured under an operating microscope (7-30x magnification).

[Study in vitro on p16beta interfering the cell cycle signal conduction of laryngeal squamous cell carcinoma].

Objective: To investigate the effect of p16beta interfering with the signal conduction of Hep-2 cell cycle in Vitro.

Methods: AdEasy Vector System was used to construct the recombinant adenovirus vector AdEasy-GFP-p1613. The recombinant adenovirus vector could introduce p16beta gene into HEK 293 cell.

[Construction of the recombinant adenovirus vector with EGFR antisense cDNA of controlling cell cycle].

Objective: To recombine the adenovirus vector carrying EGFR sence/antisense cDNA which takes part in control of cell cycle.

Methods: The 1032 bp EGFR sence/antisense cDNA fragment was cloned into the shuttle plasmid pAdTrack-CMV. The resultant plasmid and the backbone plasmid pAdEasy-1 were transferred into E.

[Construction of recombinant adenovirus vector carrying cell cycle controlling gene-p14ARF].

Objective: The recombinant adenovirus vector carrying p14ARF gene was constructed for using in the interference therapy in signal transduction of laryngeal squamous cell carcinoma.

Methods: The total cDNA fragment of p14ARF was cloned into the shuttle plasmid pAdTrack-CMV, with the resultant plasmid and the backbone plasmid pAdEasy-1, the homologous recombination took place in the E.Coli BJ5183 and the recombinant adenoviral plasmid was generated.

[Clinical anatomy measurement of accessory nerve in neck dissection].

Objective: To investigate the relations between accessory nerve and its surrounding structures.

Methods: One hundred and thirty six patients were divided into two groups: has or has no neck surgical history. Neck dissection were performed and the four distance were measured simultaneously.

[Anatomic study of pterygopalatine fossa under endoscope].

Objective: To provide endoscopic anatomic bony structures of pterygopalatine fossa for skull base surgery.

Methods: The bony structures of the pterygopalatine fossa were observed in ten dry skulls under endoscope.

Results: The pterygopalatine fossa showed a long and narrow cleft composed of the body and pterygoid process of sphenoid bone, the lamina perpendicular of palatine bone, and the posterior wall of maxillary sinus.

[Effects of budesonide, desloratadine and dexamethasone on interleukine-4 release and expression from human mast cell line].

Objective: Since human mast cell is an important source of cytokines, it is of importance to understand the effects of anti-allergic drugs on cytokines modulation in mast cells. In the present study, we aimed at observing whether IL-4 could be released from human mast cell line (HMC-1) after the stimulation of PMA + A23187, and the effects of systemic glucocorticosteroid, dexamethasone, topical glucocorticosteroid, budesonide and H1 antagonist, desloratadine on IL-4 release and mRNA expression.

Methods: HMC-1 was stimulated with 25 ng/ml phorbol 12-myristate 13-acetate (PMA) and 2.

[Experimental study of cartilage reconstruction using directed inducing MSCs-alginate complex].

Objective: To evaluate the practicability of cartilage reconstruction using directed inducing bone marrow stem cells(MSCs)- alginate complex.

Methods: The MSCs were seperated by gradient centrifugation on Percoll, then were induced into chon-drogenic differentiation. After 14 days,immunohistochemical techique was applied to detect the expression of collagen type II.

[Establishment of a modified intranasally ovalbumin induced animal model of allergic rhinitis].

Objective: To observe the early and late symptomatic, pathological and immunological changes in an intranasal ovalbumin-induced animal model of allergic rhinitis in guinea pigs.

Methods: Guinea pigs were intranasally sensitized with ovalbumin absorbed on aluminum hydroxide and after 5 days' interval, they were challenged with 1% ovalbumin solution once every 3 days for total 11 times. Two control groups were studied in parallel, the positive treatment control group was treated with antihistamine and the negative control group was sham-sensitized and sham-challenged.

[Research of the electrophysiological properties of acutely dissociated spiral ganglion neurons isolated from mice using patch clamp technique].

Objective: To explore the electrophysiological properties of spiral ganglion neurons (SGN) isolated from neonatal mice.

Methods: Ion channel currents of acutely trypsin-dissociated spiral ganglion neurons was recorded and analyzed using whole-cell variation of the voltage clamp technique.

Results: In the membrane of SGN, we recorded tetraethylammonium (TEA)-sensitive and 4-aminopyridine (4-AP)-sensitive outside delayed rectifier potassium currents, A-type potassium currents and tetrodotoxin-sensitive inside sodium currents.

[Combination radiation and gene therapy for head and neck squamous cell carcinoma in the murine model].

Objective: To examine the antitumour efficacy and investigate immunological mechanism of combination therapy of IL-2 gene and IL-12 gene transfer with radiation in an immunocompetent murine model that parallel more closely the clinical therapy of head and neck squamous cell carcinoma (HNSCC).

Methods: Tumors were established in the floor of mouth in C3H/HeJ mice with SCCVII cell line. Lipid-DNA complexed (lipoplexes) by using polycationic liposome-Mediated transduction for HNSCC was transduced in tumor-bearing mouse by direct intratumoral gene transfer.

[Establishment of methods of mechanical dissociation and culture of spiral ganglion neurons in postnatal mice].

Objective: To conduct a study on the methods for establishing a cytological model of mechanical dissociation and culture of spiral ganglion neurons (SGNs) in postnatal mice.

Methods: The spiral ganglion neurons were taken from 1-6-day old mice for primary culture. The morphology and the process of axon were observed by use of an inverted/phase contrast microscope.

[Expression of vascular endothelial growth factor C in laryngeal squamous carcinoma and its role in lymphatic metastasis].

Objective: To study the expression of vascular endothelial growth factor C (VEGF-C) in laryngeal squamous carcinoma (LSC) and its relationship to lymph node metastasis.

Methods: Paraffin-embedded specimens of primary laryngeal carcinomas from 60 patients subjected to operation were studied. VEGF-C expression was investigated using immunohistochemical staining.

[Measurement of sound pressure level at outlet of external auditory canal and eardrum].

Objective: To evaluate the sound pressure level at outlet of external auditory canal and eardrum, and to observe the range of maximum frequency of sound effect of outlet ear canal, so as to provide exact data for designing personal noise rating instrument and studying the effect of environmental noise on human body, and to provide basis for calibration of noise sound pressure level.

Method: Sound pressure level at eardrum and outlet of external auditory canal in 30 young people (60 ears) were measured by Audio Scan RM500 at 50 dB SPL narrow band noise with different frequencies (0.25 - 8.

[A pathologic analysis on 9,666 cases of tumors of nose, pharynx and throat].

Objective: To analyze nasal, pharyngeal and laryngeal tumor patients age, sex and tumors' sites, to find out the relationship of above indices and tumor's pathologic types.

Methods: 9,666 cases of tumors on nose, pharynx and throat diagnosed in West-China Hospital in 1984 to 1999 were analyzed for the constitution of histological type.

Results: 1.