Publications by authors named "Chuan-Zhen Zhang"
Emergence of ciprofloxacin heteroresistance in foodborne Salmonella enterica serovar Agona.
J Antimicrob Chemother
October 2020
Article Synopsis
- Bacterial heteroresistance is becoming a significant issue, notably with antibiotic-certain bacterial combinations, and this study focuses specifically on ciprofloxacin heteroresistance in Salmonella.
- The research used various methods, including population analysis and whole-genome sequencing, to identify specific mutations and gene expressions linked to ciprofloxacin resistance in Salmonella isolated from food.
- Findings revealed that ciprofloxacin heteroresistance stems from mutations in target genes and overexpression of efflux pumps, highlighting a concern for antibiotic efficacy in foodborne pathogens and the importance of accurately interpreting antibiotic susceptibility.
Article Synopsis
- The study investigates the prevalence of CTX-M-producing isolates from raw meat and food animals, highlighting a 17.76% occurrence of ESBL-positive isolates.
- Among the findings, CTX-M-55 was the most common type, with 44.44% of ESBL-positive isolates carrying it, predominantly from food animals.
- The research indicates a significant public health threat due to the co-existence of fluoroquinolone resistance genes and the spread of these resistant organisms through the food chain in China.*
The aim of this study was to investigate the difference in resistance mechanisms and fitness of Salmonella Typhimurium (ST) and Salmonella Enteritidis (SE) mutants selected during the evolution of resistance under exposure to increasing ciprofloxacin concentrations in vitro. Mutations in quinolone target genes were screened by PCR. Phenotypic characterization included susceptibility testing by the broth dilution method, investigation of efflux activity and growth rate, and determination of the invasion of human intestinal epithelium cells in vitro.
View Article and Find Full Text PDFThe aim of this study was to determine the expression of eight other functional transporter genes upon acrAB inactivation and also the expression of acrAB when the function of eight other transporters are impaired in Salmonella enterica. We used single- or multigene deletion mutants (i.e.
View Article and Find Full Text PDFWe studied mechanisms of drug resistance development in Escherichia coli strains lacking efflux pump components. E. coli K12 deletion mutants were subjected to increasing concentrations of ciprofloxacin (CIP) to determine the frequency of target gene mutations.
View Article and Find Full Text PDFDifference in the development of resistance may be associated with the epidemiological spread and drug resistance of different Salmonella enterica serovar strains. In the present study, three susceptible S. enterica serovars, Typhimurium (ST), Enteritidis (SE), and Indiana (SI) strains, were subjected to stepwise selection with increasing ciprofloxacin concentrations.
View Article and Find Full Text PDFArticle Synopsis
- The study aimed to explore how genetic variations in the RAD51 gene might influence the risk of developing esophageal squamous cell carcinoma (ESCC).
- 316 ESCC patients and 316 healthy controls in China were analyzed for three specific single nucleotide polymorphisms (SNPs) using advanced genetic testing methods.
- Results indicated that while individual SNPs didn't show a significant link to ESCC risk, the CGG haplotype derived from these SNPs was associated with an increased risk of the disease.
Background And Objective: Mutations in DNA repair system are related to carcinogenesis. This study was to evaluate the correlations of polymorphisms and haplotypes of XPD gene with individual susceptibility to gastric cancer.
Methods: Genomic DNA were extracted from peripheral blood leukocytes of 207 gastric cancer patients and 212 healthy controls.