Circular RNA from Tyrosylprotein Sulfotransferase 2 Gene Inhibits Cisplatin Sensitivity in Head and Neck Squamous Cell Carcinoma by Sponging miR-770-5p and Interacting with Nucleolin.

Chemoresistance poses a significant challenge in the treatment of advanced head and neck squamous cell cancer (HNSCC). The role and mechanism of circular RNAs (circRNAs) in HNSCC chemoresistance remain understudied. We conducted circRNA microarray analysis to identify differentially expressed circRNAs in HNSCC.

Molecular targets of primary cilia defects in cancer (Review).

Primary cilia are hair‑like organelles that are present on the majority of mammalian cells. They are regarded as the regulatory 'hub' of cell functions due to their indispensable roles for several signaling pathways, such as Hh and Wnt pathways. Originally, cilia defects were found to cause a panoply of human diseases commonly referred to as 'ciliopathies'.

Roles of circRNAs in cancer chemoresistance (Review).

Circular RNA (circRNA) is a type of endogenous, high‑stability, noncoding RNA. circRNAs exhibit various biological functions, and are involved in physiological and pathological processes occurring in various diseases, including cancers. They can not only act as microRNA and protein sponges, but also interact with proteins, translated peptides, and transcriptional and translational regulators, and compete with pre‑mRNA splicing.

PA28γ, an Accomplice to Malignant Cancer.

PA28γ is a nuclear activator of the 20S proteasome, which is involved in the regulation of several essential cellular processes and angiogenesis. Over the past 20 years, many amino acid sites and motifs have been proven to play important roles in the characteristic functions of PA28γ. The number of binding partners and validated cellular functions of PA28γ have increased, which has facilitated the clarification of its involvement in different biological events.

[Analysis of potential pharmacodynamic material basis of Euonymus alatus in treatment of nephritis based on integrated chemomics and network biology].

This paper was aimed to explore the potential pharmacodynamics effect of Euonymus alatus in the treatment of nephritis based on integrated chemomics and network biology. The chemical constituent database of E. alatus was constructed by consulting litera-ture and using online database.

Mapping of Extrinsic Innervation of the Gastrointestinal Tract in the Mouse Embryo.

Precise extrinsic afferent (visceral sensory) and efferent (sympathetic and parasympathetic) innervation of the gut is fundamental for gut-brain cross talk. Owing to the limitation of intrinsic markers to distinctively visualize the three classes of extrinsic axons, which intimately associate within the gut mesentery, detailed information on the development of extrinsic gut-innervating axons remains relatively sparse. Here, we mapped extrinsic innervation of the gut and explored the relationships among various types of extrinsic axons during embryonic development in mice.

Noncoding RNAs in oral premalignant disorders and oral squamous cell carcinoma.

Background: Oral squamous cell carcinoma (OSCC) has the highest mortality rate among all head and neck cancers and a relatively low five-year survival rate. Generally, the development of an oral mucosal malignancy represents a multistep process beginning with normal oral mucosa epithelium and culminating in OSCC after transitioning through intermediary oral premalignant disorders (OPMDs), during which dysplasia is often observed. Noncoding RNAs (ncRNAs) are RNAs that are not translated into proteins, but still can participate in regulating neoplastic cell behavior.

[Construction of an oral squamous cell carcinoma cell line for stable PA28γ overexpression].

Objective: To construct a PA28γ overexpression cell line and determine its effects after infecting an oral squa-mous cell carcinoma (OSCC) cell line.

Methods: The PA28γ gene was cloned into the pLOV.CMV.

MicroRNA-302b negatively regulates IL-1β production in response to MSU crystals by targeting IRAK4 and EphA2.

Background: Interleukin-1β (IL-1β) is a pivotal proinflammatory cytokine that is strongly associated with the inflammation of gout. However, the underlying mechanism through which the production of IL-1β is regulated has not been fully elucidated. Our previous work identified that miR-302b had an important immune regulatory role in bacterial lung infections.

[Preliminary research on prokaryotic expression and immune protection of triosephosphate isomerase of ].

Objective: To study the prokaryotic expression and immune protection of triosephosphate isomerase (TPI) of in mice.

Methods: Total RNA was extracted from toxoplasma tachyzoites, and TPI fragment was amplified by PCR and cloned into the prokaryotic expression vector pET-28a (+). The target protein was induced with IPTG and purified by Ni-NTA affinity chromatography.

[Progress of researches on diagnostic methods of current infection].

Now schistosomiasis is still a serious zoonosis which affects human health and hinders the economy development in endemic areas. Accurate diagnosis of the infection of is very significant in reducing hazards to human health and controlling the epidemic of schistosomiasis. This review summarizes recent advances in the laboratory diagnostic methods for current schistosome infection (including pathogenic, immunologic and molecular biologic methods) so as to provide the reference for prevention and control of schistosomiasis in the field.

Resection and drainage of hilar cholangiocarcinoma: an 11-year experience of a single center in mainland China.

The purpose of this study is to provide appropriate approaches for resection and drainage of hilar cholangiocarcinomas. Surgical approaches and postoperative survival rates of the patients were analyzed retrospectively. The 1-, 3-, and 5-year cumulative survival rates for patients who underwent resection were 76.