Hyperbaric Oxygen Treatment for Chronic Insomnia at High Altitude: A Prospective, Randomized, Open-Label, Parallel-Group Trial.

Background: Hyperbaric oxygen (HBO) has been proposed to be effective for treating chronic insomnia at high altitudes.

Methods: Patients suffering from chronic insomnia at high altitude were randomly assigned at a 1:1 ratio to either the hyperbaric oxygen (HBO) treatment group or the control group for a 10-day procedure. The primary outcome was insomnia status, which was assessed via the Pittsburgh Sleep Quality Index (PSQI) and the Insomnia Severity Index (ISI).

Open Scapulothoracic Dissociation.

Scapulothoracic dissociation is a rare and serious type of trauma caused by musculoskeletal, vascular, and brachial plexus injuries owing to lateral displacement of the scapula with a strong blunt external force. Here, we describe the treatment of an open scapulothoracic dissociation patient and summarize the findings.

Lattice QCD Calculation of the Subtraction Function in Forward Compton Amplitude.

The subtraction function plays a pivotal role in calculations involving the forward Compton amplitude, which is crucial for predicting the Lamb shift in muonic atoms, as well as the proton-neutron mass difference. In this Letter, we present a lattice QCD calculation of the subtraction function using two domain wall fermion gauge ensembles near the physical pion mass. We utilize a recently proposed subtraction point, demonstrating its advantage in mitigating statistical and systematic uncertainties by eliminating the need for ground-state subtraction.

Noninvasive Quantification of Hepatic Steatosis Using Ultrasound-Derived Fat Fraction (CHESS2303): A Prospective Multicenter Study.

Ultrasound-derived fat fraction (UDFF) is designed to assess the hepatic fat content quantitatively. A multicenter study that verifies the diagnostic performance of UDFF for detecting hepatic steatosis has not yet been reported. This study aimed to evaluate the performance of UDFF for diagnosing and grading hepatic steatosis.

Spatial single-cell proteomics landscape decodes the tumor microenvironmental ecosystem of intrahepatic cholangiocarcinoma.

Background Aims: The prognoses and therapeutic responses of patients with intrahepatic cholangiocarcinoma (iCCA) depend on spatial interactions among tumor microenvironment (TME) components. However, the spatial TME characteristics of iCCA remain poorly understood. The aim of this study was to generate a comprehensive spatial atlas of iCCA using artificial intelligence-assisted spatial multiomics patterns and to identify spatial features associated with prognosis and immunotherapy.

Methionine Restriction Exerts Anti-Tumor Immunity via Joint Intervention of T-Bet Palmitoylation in Gastric Cancer.

Background: Methionine restriction (MR) exerts an anti-tumor immunomodulatory role. Th1 cells facilitate CD8 cytotoxic T cell activation and targeted tumor cell killing. Our previous work shows that MR enhances the immunotherapy effect of PD-L1/PD-1 blockade on gastric cancer, MR can simultaneously inhibit Th1 cell differentiation, which may affect their synergistic therapeutic outcome.

Associations between pre- and post-diagnosis dietary inflammatory patterns and ovarian cancer survival: results from the Ovarian Cancer Follow-Up Study.

Background: Dietary factors impact systemic inflammation, which not only correlates with poorer outcomes in patients with ovarian cancer (OC) but also promotes cancer development through increased cell division, genetic alterations, and malignant transformation of epithelial cells at inflammatory sites. However, evidence between dietary inflammatory patterns and OC survival remains sparse.

Objective: The aim of this study was to examine associations between pre- and post-diagnosis dietary inflammatory patterns, including their changes, and overall survival (OS).

EPS-8 regulates human malignant melanoma development by activating the Hedgehog pathway via degradation of Ptch1.

Background: The epidermal growth factor receptor pathway substrate 8 (EPS-8) is a tumor-associated antigen that is frequently overexpressed in various types of human solid tumors and is associated with aggressiveness and poor prognosis. The role of EPS-8 in cutaneous malignant melanoma and its potential mechanism remains unknown.

Methods: EPS-8 expression, mutation immune infiltration, and the tumor microenvironment in melanoma were analyzed using various databases.

Opportunistic screening for bone mineral density deficits in pediatric patients from abdominal computed tomography scans obtained for other indications: a cross-sectional study.

Background: Quantitative computed tomography (QCT) has been widely applied for opportunistic screening for osteoporosis in adults, but it is rarely used in pediatric patients.

Objective: This study aimed to investigate the feasibility of QCT and the prevalence of bone mineral density (BMD) deficits at the spine in pediatric patients undergoing an abdominal computed tomography (CT) for other indications.

Materials And Methods: Pediatric patients who underwent a clinical abdominal CT scan from October 2018 to November 2020 were recruited for this retrospective cross-sectional study.

Quantum-Dot-Electrolyte Light-Emitting Diodes for Displays.

Electroluminescence (EL) is essential for modern technologies, such as displays, lighting, and optical communications. To date, some kinds of artificial EL devices have been developed, including organic light-emitting diodes (OLEDs), quantum-dot (QD) LEDs, and light-emitting electrochemical cells. However, issues (e.

Cancer Cell and Cancer-Associated Fibroblast Communication-Mediated Molecular Subtypes Portray Non-Inflamed Tumor Microenvironment and Guide the Precision Treatment of Bladder Cancer.

Cancer-associated fibroblasts (CAFs) drive tumor progression through restructuring of the tumor microenvironment. This investigation aim to elucidate the function of molecular subtypes (MS) derived from cancer cells communication with CAFs, depicting the hallmarks of the tumor microenvironment and precise bladder cancer (BLCA) treatment. The BLCA data from TCGA and several external sources are utilized to generate a novel ligand, receptor, and transcription factor (LRT) associated molecular subtype and their corresponding score (LRT score).

Caffeine intake associated with a lower risk of liver fibrosis in different glucose status.

Background And Aims: To investigate the prevalence of liver fibrosis, and the association between caffeine intake and fibrosis in populations with different glucose metabolism status.

Methods: This was a cross-sectional study based on the National Health and Nutrition Examination Survey (2005-March 2020). Of the 39,221 adult individuals with no necessary laboratory results missing, a total of 23,711 eligible individuals were included in the study.

Multiple-omics analysis reveals a dedifferentiation-immune loop in intrahepatic cholangiocarcinoma.

Intrahepatic cholangiocarcinoma (ICC) is known for its diverse cell types and resistance to standard treatments, highlighting the importance of understanding its tumor microenvironment (TME) for improved prognostic accuracy and therapeutic innovation. Our study used a multi-omics approach to analyze the ICC TME in both human and mouse samples, linking survival outcomes to the complex cellular interactions within the TME. We discovered a dedifferentiation phenomenon in ICC cells driven by the Yes-associated protein (YAP) pathway, influenced by tumor-associated macrophages (TAMs).

Phytochemicals Targeting Mitophagy to Treat Heart Diseases: Retrospective Insights and Prospective Directions.

Mitophagy is a process by which cells selectively eliminate damaged or dysfunctional mitochondria through the autophagy-lysosome pathway, thereby maintaining mitochondrial quality and cellular homeostasis. This process is closely linked to the onset and progression of various heart diseases. Modern pharmacological research has demonstrated that phytochemicals can regulate mitochondrial homeostasis in cardiomyocytes through multiple mechanisms, influencing mitophagy and protecting cardiomyocytes, which in turn exerts anti-cardiovascular effects.

FIB-4plus Score: A novel machine learning-based tool for screening high-risk varices in compensated cirrhosis (CHESS2004): An international multicenter study.

Background/aims: A large percentage of patients undergoing esophagogastroduodenoscopy (EGD) screening do not have esophageal varices (EV) or have only small EV. We evaluated a large, international, multicenter cohort to develop a novel score, termed FIB-4plus, by combining the fibrosis-4 (FIB-4) score, liver stiffness measurement (LSM), and spleen stiffness measurement (SSM) to identify high-risk EV (HRV) in compensated cirrhosis.

Methods: This international cohort study involved patients with compensated cirrhosis from 17 Chinese hospitals and one Croatian institution (NCT04546360).

A portable EEG signal acquisition system and a limited-electrode channel classification network for SSVEP.

Brain-computer interfaces (BCIs) have garnered significant research attention, yet their complexity has hindered widespread adoption in daily life. Most current electroencephalography (EEG) systems rely on wet electrodes and numerous electrodes to enhance signal quality, making them impractical for everyday use. Portable and wearable devices offer a promising solution, but the limited number of electrodes in specific regions can lead to missing channels and reduced BCI performance.

Cianidanol from Sea Buckthorn Exert Anti-Inflammatory Effects by the Inhibiting JAK2/STAT3 Signaling Pathway via an Integrative Pharmacology Strategy.

Sea buckthorn is a model of medicine and food homology, but the chemical composition and mechanism of anti-inflammatory effects are limited. In this study, the key components and mechanisms of the anti-inflammatory effects of sea buckthorn were identified based on UPLC-Q-TOF-MS, network pharmacology, molecular docking, molecular dynamics and RAW264.7 cells.

Global, regional, and national burden of very early-onset colorectal cancer and its risk factors from 1990 to 2019: A systematic analysis for the global burden of disease study 2019.

Aims: Very early-onset colorectal cancer (EOCRC) was defined as CRC diagnosed before the age of 35 proposed by the latest EOCRC management guideline. Until now, the disease burden of very EOCRC has never been reported. This study aimed to explore the burden of very EOCRC across the past three decades.

In situ architecture of a nucleoid-associated biomolecular co-condensate that regulates bacterial cell division.

In most bacteria, cell division depends on the tubulin-homolog FtsZ that polymerizes in a GTP-dependent manner to form the cytokinetic Z-ring at the future division site. Subsequently, the Z-ring recruits, directly or indirectly, all other proteins of the divisome complex that executes cytokinesis. A critical step in this process is the precise positioning of the Z-ring at the future division site.

Unveiling the molecular mechanism of sepal curvature in Section through full-length transcriptome and RNA-seq analysis.

Introduction: Orchids are renowned for their intricate floral structures, where sepals and petals contribute significantly to ornamental value and pollinator attraction. In Section , the distinctive curvature of these floral organs enhances both aesthetic appeal and pollination efficiency. However, the molecular and cellular mechanisms underlying this trait remain poorly understood.

[Clinical treatment strategy for pT3N0 laryngeal squamous cell carcinoma].

To investigate optimal treatment strategy for pT3N0 laryngeal squamous cell carcinoma（SCC）. A retrospective study of 150 patients with pT3N0 laryngeal SCC treated in the First Affiliated Hospital of Chongqing Medical University was performed. The efficacies of partial laryngectomy and total laryngectomy, as well as surgery alone and postoperative radiotherapy were evaluated.

Rabeprazole inhibits lung cancer progression by triggering NLRP3/CASP-1/caspase-dependent pyroptosis.

Background: Gastric acid-related diseases could be treated using proton pump inhibitors (PPIs), which have been found to have anti-tumor ability. Rabeprazole is a type of PPI whose effect and mechanism in lung cancer remained to be clarified.

Methods: Lung cancer cells and lung cancer mice were treated with different concentrations of Rabeprazole and then cell proliferation was detected by CCK-8 and colony formation assays.

Beyond Dimerization: Harnessing Tetrameric Coiled-Coils for Nanostructure Assembly.

Versatile DNA and polypeptide-based structures have been designed based on complementary modules. However, polypeptides can also form higher oligomeric states. We investigated the introduction of tetrameric modules as a substitute for coiled-coil dimerization units used in previous modular nanostructures.

Regulation of AUF1 alternative splicing by hnRNPA1 and SRSF2 modulate the sensitivity of ovarian cancer cells to cisplatin.

Purpose: Clarification of cisplatin resistance may provide new targets for therapy in cisplatin resistant ovarian cancer. The current study aims to explore involvement of isoforms of AU-rich element RNA-binding protein 1 (AUF1) in cisplatin resistance in ovarian cancer.

Methods: The cancer stem cell-like features were analyzed using colony formation assay, tumor sphere formation assay and nude mouse xenograft experiments.