A molecular mechanism underlies grass carp (Ctenopharyngodon idella) TARBP2 regulating PKR-mediated cell apoptosis.

Interferon-inducible double-stranded RNA-dependent protein kinase (PKR) is one of the key antiviral arms in the innate immune system. The activated PKR performs its antiviral function by inhibiting protein translation and inducing apoptosis. In our previous study, we identified grass carp TARBP2 as an inhibitor of PKR activity, thereby suppressing cell apoptosis.

An effective integrated design for enhanced cathodes of Ni foam-supported Pt/carbon nanotubes for Li-O2 batteries.

Designing an effective microstructural cathode combined with a highly efficient catalyst is essential for improving the electrochemical performance of Li-O2 batteries (LOBs), especially for long-term cycling. We present a nickel foam-supported composite of Pt nanoparticles (NPs) coated on self-standing carbon nanotubes (CNTs) as a binder-free cathode for LOBs. The assembled LOBs can afford excellent electrochemical performance with a reversible capacity of 4050 mAh/g tested at 20 mA/g and superior cyclability for 80 cycles with a limited capacity of 1500 mAh/g achieved at a high current density of 400 mA/g.

Overexpression of Hsp90 from grass carp (Ctenopharyngodon idella) increases thermal protection against heat stress.

With homologous DNA probes, we had screened a grass carp heat shock protein 90 gene (CiHsp90). The full sequence of CiHsp90 cDNA was 2793 bp, which could code a 798 amino acids peptide. The phylogenetic analysis demonstrated that CiHsp90 shared the high homology with Zebrafish Grp94.

Cloning and functional analysis of PKZ (PKR-like) from grass carp (Ctenopharyngodon idellus).

The new teleost fish PKZ (PKR-like) full-length cDNA (GU299765) had been cloned and identified from grass carp (Ctenopharyngodon idellus). The cDNA of grass carp PKZ (CiPKZ) has 2185 bp in length with a largest open reading frame (ORF) encoding 513aa. CiPKZ possesses a conserved C-terminal catalytic domain of eIF2α kinase family.

The Zalpha domain of PKZ from Carassius auratus can bind to d(GC)(n) in negative supercoils.

PKZ was the most recently discovered member of eIF2alpha kinase family in fish. CaPKZ, the first identified fish PKZ, possessed a conserved eIF2alpha kinase catalytic domain in C-terminal and two Z-DNA binding domains (Zalpha) in N-terminal. The Zalpha of CaPKZ closely resembled that of other Z-DNA binding proteins: ADAR1, DLM-1, and E3L.