Publications by authors named "Chu Ling Guo"
The first step of biodegradation is the contact of microorganism and pollutants, in order to examine the influence of phenanthrene on Sphingomonas sp. GY2B's surface properties during its degrading process, the bacteria was cultivated at different conditions, and detected by flow cytometry combined with fluorescent dyes for its surface changes. The results indicated that, the membrane structure had been certainly damaged during the degrading process, leading to an increased membrane permeability.
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- The study investigates how the Bacillus cereus RC-1 bacterium protects itself from different levels of cadmium toxicity, focusing on its growth, cadmium absorption, and cellular structure.
- Analysis techniques like SEM, AFM, TEM, and UV spectrophotometry revealed that cadmium concentrations above 20 mg L(-1) significantly disrupt bacterial growth and cellular structure.
- The findings indicate that Bacillus cereus RC-1 predominantly absorbs cadmium internally at lower concentrations (under 20 mg L(-1)), but relies more on external adsorption at higher concentrations, suggesting potential for bioremediation in environments with lower cadmium levels.
This work aims at preparing oil spill sorbent (TCS, Trichoderma viride-modified corn stalk) through solid-state fermentation of corn stalk by Trichoderma viride. Single-factor experiments, including the effect of modification time, solid-liquid ratio of modification and modification temperature, and adsorption experiments simulating oil spill condition, were carried out. The results indicated that the maximum oil adsorption of TCS, 13.
View Article and Find Full Text PDFBiosorption of Cd(II) by live and dead cells of Bacillus cereus RC-1 isolated from cadmium-contaminated soil.
Colloids Surf B Biointerfaces
July 2013
The present study investigated the biosorption capacity of live and dead cells of Bacillus cereus RC-1 for Cd(II). The biosorption characteristics were investigated as a function of initial pH, contact time, and initial cadmium concentration. Equilibrium biosorption was modeled using Langmuir, Freundlich and Redlich-Peterson isotherm equations.
View Article and Find Full Text PDFThe effect of rhamnolipids on pyrene degradation by Pseudomonas sp. GP3A was investigated to explore the interrelationships of biosurfactant, hydrophobic substrate and degrading bacteria. The cell surface hydrophobicity, lipopolysaccharide, hydrogen bond, bacterial biomass and pyrene degradation were determined.
View Article and Find Full Text PDFThe promotion of hexadecane biodegradation activity by an n-alkane degrading strain of Burkholderia cepacia (GS3C) with yeast extract amendment was studied using various carbon, nitrogen, vitamin, and amino acid amendments. Cytochrome P450 monooxygenase enzymes play a very important role and are especially required to introduce oxygen in n-alkane degradation. These enzymes from GS3C were located and detected using amino acid amendments.
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- A study isolated 32 bacterial strains from mangrove sediments to explore diversity in dioxygenase genes linked to degrading polycyclic aromatic hydrocarbons (PAHs).
- Two types of PCR primers (nidA-like and nahAc-like) were created and successfully amplified DNA fragments from both Gram-positive and Gram-negative bacteria, indicating the presence of specific genes associated with PAH degradation.
- The findings revealed three subtypes of the nidA-like gene and significant similarities between the detected nahAc-like sequences and known dioxygenases, highlighting the prevalence of these genes in PAH-degrading bacteria and their potential use in environmental assessments.