Publications by authors named "Christy Muhlen"

Hot water systems are the most frequent environment associated with the prevalence and growth of opportunistic premise plumbing pathogens (OPPPs). Previous studies identified water heaters as a source of waterborne diseases and concluded that design variables may contribute to their prevalence. A multifaceted approach was used to investigate the vertical stratification of the microbiome and selected OPPPs in an electric water heater tank connected to a home plumbing system simulator.

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Thirty-one drinking water storage tank sediment samples were collected in 13 states, 17 distribution systems, and 29 tanks over the course of 4 years. Sediment samples were characterized for elemental composition and physical properties, which were found to be inconsistent both between samples of the same distribution system and across geographical regions. Differences between samples from the same tank also indicated spatial differences in sediment composition within storage tanks.

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Chloraminated drinking water systems commonly use free chlorine conversions (FCCs) to prevent or control nitrification, but unintended water quality changes may occur, including increased disinfection by-product and metal concentrations. This study evaluated water quality in a chloraminated drinking water system and residential locations before, during, and after their annual, planned FCC. Water quality alternated between relatively consistent and variable periods when switching disinfectants.

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Opportunistic premise plumbing pathogens (OPPPs) have been detected in buildings' plumbing systems causing waterborne disease outbreaks in the United States. In this study, we monitored the occurrence of OPPPs along with free-living amoeba (FLA) and investigated the effects of residential activities in a simulated home plumbing system (HPS). Water samples were collected from various locations in the HPS and analyzed for three major OPPPs: , nontuberculous mycobacterial species (e.

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Hard water and elevated ammonia are problems for many United States groundwater drinking water utilities, and some utilities, particularly those in the Midwest, face both challenges. Ion (cation) exchange (IX) is a common treatment technique for hardness reduction (i.e.

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Orthophosphate (PO) addition is a common corrosion control treatment used to lower lead (Pb) concentrations at the consumer's tap by forming relatively insoluble Pb-phosphate (Pb-PO) minerals. However, some Pb-PO particles that can form in drinking water are mobile nanoparticles (i.e.

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There are many sampling approaches available for lead (Pb) in drinking water. Selecting the best approach for its intended use is critical. The objective of this work was to compare water Pb levels collected by multiple sampling approaches from a model home plumbing system (HPS) that included an old Pb service line (LSL), as a function of daily water usage.

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Although not regulated in United States drinking water, ammonia has the potential to increase chlorine consumption and cause nitrification problems in the distribution system. Many groundwaters with elevated ammonia are also contaminated with other inorganic analytes such as arsenic, iron, and manganese, all of which have primary or secondary maximum contaminant levels (MCLs). The objective of this work was to demonstrate the effectiveness of an innovative biological treatment process to simultaneously remove ammonia (2.

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Opportunistic pathogens such as Legionella are of significant public health concern in hospitals. Microbiological and water chemistry parameters in hot water throughout an Ohio hospital were monitored monthly before and after the installation of a monochloramine disinfection system over 16 months. Water samples from fifteen hot water sampling sites as well as the municipal water supply entering the hospital were analyzed using both culture and qPCR assays for specific microbial pathogens including Legionella, Pseudomonas spp.

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There is great interest in producing nanoparticles for various applications. The objective of this work was to develop a procedure for reproducibly creating stable lead (Pb) phosphate nanoparticle aqueous suspensions. A stable 5 mg/L Pb-phosphate nanoparticle "stock" suspension was synthesized via chemical precipitation of Pb and orthophosphate in water at pH 7.

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Retrospective analysis of 20 water systems from the USEPA's Arsenic Demonstration Program revealed three patterns of arsenic levels at the tap, after arsenic treatment of the source well water. Following an initial destabilization period, Pattern A systems (6/20 with low iron/manganese in source water and plastic piping) had arsenic concentrations that did not change as water traveled to consumer taps (conservative contaminant behavior). Pattern B systems (8/20 with high iron/manganese in source water and iron piping) had consistently higher arsenic concentrations at consumer taps, above the arsenic content of incoming treated water, for months to more than a year after arsenic treatment (non-conservative behavior).

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The US Environmental Protection Agency's Office of Research and Development designed, fabricated, and installed four pipe rigs in Flint, Mich., to help the city optimize corrosion control. The lead service line (LSL) pipe loops were constructed of polyvinyl chloride pipe and fittings and welded steel channel frames.

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Legionella pneumophila (Lp) is ubiquitous in the aquatic environment and can persist within drinking water distribution systems (DWDS) enabling these systems to serve as a potential source of human infections. Bacterial surface charge, deduced from electrophoretic mobility (EPM), is a well-recognized contributor to microorganism mobility, adherence and interactions with their surrounding environment. In this study, the EPM of 32 Lp strains representing serogroup (sg) 1 to 14 were measured, in 9.

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Tap water sampling and surface analysis of copper pipe/bathroom porcelain were performed to explore the fate of copper and silver during the first nine months of copper-silver ionization (CSI) applied to cold and hot water at a hospital in Cincinnati, Ohio. Ions dosed by CSI into the water at its point of entry to the hospital were inadvertently removed from hot water by a cation-exchange softener in one building (average removal of 72% copper and 51% silver). Copper at the tap was replenished from corrosion of the building's copper pipes but was typically unable to reach 200 μg/L in first-draw and flushed hot and cold water samples.

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