Crafting Molecular Tools for 15-Lipoxygenase-1 in a Single Step.

Small molecule modulators are powerful tools for selectively probing and manipulating proteins in native biological systems. However, the development of versatile modulators that exhibit desired properties is hindered by the lack of a rapid and robust synthetic strategy. Here, we develop a facile and reliable one-step methodology for the generation of multifunctional toolboxes encompassing a wide variety of chemical modulators with different desired features.

Investigating the Catalytic Site of Human 15-Lipoxygenase-1 via Marine Natural Products.

Human 15-lipoxygenase-1 (15-LOX-1) is a key enzyme that possesses an important role in (neuro)inflammatory diseases. The pocket of the enzyme plays the role of a chiral catalyst, and therefore chirality could be an important component for the design of effective enzyme inhibitors. To advance our knowledge on this concept, we developed a library of the identified chiral 15-LOX-1 inhibitors and applied cheminformatic tools.

A versatile micromodel technology to explore biofilm development in porous media flows.

Bacterial biofilms that grow in porous media are critical to ecosystem processes and applications ranging from soil bioremediation to bioreactors for treating wastewater or producing value-added products. However, understanding and engineering the complex phenomena that drive the development of biofilms in such systems remains a challenge. Here we present a novel micromodel technology to explore bacterial biofilm development in porous media flows.

NEURD offers automated proofreading and feature extraction for connectomics.

We are now in the era of millimeter-scale electron microscopy (EM) volumes collected at nanometer resolution. Dense reconstruction of cellular compartments in these EM volumes has been enabled by recent advances in Machine Learning (ML). Automated segmentation methods produce exceptionally accurate reconstructions of cells, but post-hoc proofreading is still required to generate large connectomes free of merge and split errors.

Prevalence and Predictors of COVID-19 Vaccination Acceptance among Greek Health Care Workers and Administrative Officers of Primary Health Care Centers: A Nationwide Study Indicating Aspects for a Role Model.

Background: Τhe study aims to identify factors associated with COVID-19 vaccine acceptance and to investigate knowledge and perceptions of Primary Health Care Centers (PHCC) personnel, who acted as pioneers in the national COVID-19 vaccination strategy. Methods and Materials: A nationwide cross-sectional survey was conducted by distributing an online anonymous questionnaire comprising 25 questions during the first semester of 2021. Results: Approximately 85.

Factors Associated with Healthcare Workers' (HCWs) Acceptance of COVID-19 Vaccinations and Indications of a Role Model towards Population Vaccinations from a Cross-Sectional Survey in Greece, May 2021.

A Knowledge, Attitudes and Practices (KAP) study was conducted at the end of May 2021 engaging 1456 healthcare workers (HCWs) from 20 hospitals throughout Greece. Acceptance of vaccination against coronavirus disease 2019 (COVID-19) was estimated at 77.7%, with lower vaccine acceptance identified in nurses compared to physicians.

Intracellular targets: A multiple cargo transporting molecule.

The generation of cell-penetrating peptides as cargo-delivery systems has produced an immense number of studies owing to the importance of these systems as tools to deliver molecules into the cells, as well as due to the interest to shed light into a yet unclear mechanism of the entrance of these peptides into the cells. However, many cell-penetrating peptides might present drawbacks due to causing cellular toxicity, or due to being entrapped in endosomes, or as a result of their degradation before they meet their target. In this work, a cargo transporting molecule, the Cell Penetrating Sequential Oligopeptide Carrier (CPSOC), formed by the repetitive -Lys-Aib-Cys- moiety, was tested for its ability to penetrate the cell membrane and transport the conjugated peptides into the cells.

Host Species Determines the Composition of the Prokaryotic Microbiota in Sandflies.

Phlebotomine sandflies are vectors of the humans' and mammals' parasite spp. Although the role of gut microbiome in the biological cycle of insects is acknowledged, we still know little about the factors modulating the composition of the gut microbiota of sandflies. We tested whether host species impose a strong structural effect on the gut microbiota of spp.

Eight-year survival study of first-line tumour necrosis factor α inhibitors in rheumatoid arthritis: real-world data from a university centre registry.

Objective: This study aimed to investigate the efficacy, safety and survival of TNF-α inhibitors in patients with RA.

Methods: A total of 178 patients >18 years of age were treated with TNF-α inhibitors. A total of 74 patients were treated with infliximab, 75 with adalimumab and 29 with etanercept.

Evaluation of EU air quality standards through modeling and the FAIRMODE benchmarking methodology.

We evaluate air quality modeling over the East Mediterranean using the benchmarking methodology developed in the framework of the Forum for Air Quality Modelling in Europe (FAIRMODE). FAIRMODE aims to provide a harmonized approach of model evaluation for regulatory purposes. We test the methodology by assessing the performance of the Weather Research and Forecasting model coupled with chemistry (WRF-Chem) against ground-based air quality observations over Cyprus, a member state of the European Union.

Microbiome of peri-implantitis affected and healthy dental sites in patients with a history of chronic periodontitis.

Objective: To determine the composition of the microbiome of peri-implantitis sites and corresponding dental sites in subjects with a history of chronic periodontitis.

Design: Clinical and radiographic examination assessed the periodontal/peri-implant disease status. Plaque samples were collected from one diseased implant with peri-implantitis, functional for at least two years and healthy sites in ten non-smokers who had received periodontal treatment prior to implant placement.

Bacterial pathogens and endosymbionts in ticks.

Ticks collected from goats in northern Greece were tested for the presence of tick-borne bacteria. Among adult ticks, 37 (57.8%) were Rhipicephalus bursa, 11 (17.

Prolonged occupational exposure leads to allergic airway sensitization and chronic airway and systemic inflammation in professional firefighters.

Background And Objectives: Little data exist on short- and long-term effects of occupational exposure on airway and systemic inflammation in professional firefighters. We aimed to characterize airway and systemic inflammation in training firefighters with a maximum occupational exposure of 1 year compared to the long-term exposure of professional firefighters.

Methods: A questionnaire for symptoms and exposure, pulmonary function, atopy, bronchial hyper-responsiveness, and markers of inflammation in induced sputum, serum, bronchoalveolar lavage (BAL) fluid and bronchial biopsies were assessed in a total of 92 firefighters (63 full-time professionals and 29 trainees).

Prevalence of antibiotic resistance genes in subjects with successful and failing dental implants. A pilot study.

Objectives: To investigate the prevalence of the bacterial genes encoding resistance to beta-lactams, tetracyclines and metronidazole respectively, in subjects with successful and failing dental implants and to assess the presence of Staphylococcus aureus and the mecA gene encoding for Methicillin Resistant Staphylococcus aureus (MRSA) in the same samples.

Materials And Methodology: The subject sample included 20 participants with clinically healthy osseointegrated implants and 20 participants with implants exhibiting peri-implantitis. Clinical parameters were assessed with an automated probe, samples were collected from the peri-implant sulcus or pocket and analyzed with Polymerase Chain Reaction for bla TEM , tetM, tetQ and nim genes, S.

The utilization of a diode laser in the surgical treatment of peri-implantitis. A randomized clinical trial.

Objectives: A comparison of different treatment modalities of peri-implantitis can lead to the development and application of more effective and efficient methods of therapy in clinical practice. This study compares the effectiveness of open flap debridement used alone, with an approach employing the additional use of a diode laser for the treatment of peri-implantitis.

Materials And Methods: Nineteen patients were divided into two groups and treated for peri-implantitis.

Multiarticular isokinetic high-load eccentric training induces large increases in eccentric and concentric strength and jumping performance.

This study investigated the effects of short-term eccentric exercise training using a custom-made isokinetic leg press device, on concentric and eccentric strength and explosiveness as well as jumping performance. Nineteen healthy males were divided into an eccentric (ECC, n = 10) and a control group (CG, n = 9). The ECC group trained twice per week for 8 weeks using an isokinetic hydraulic leg press machine against progressively increasing resistance ranging from 70 to 90% of maximal eccentric force.

The effect of high vs. low carbohydrate diets on distances covered in soccer.

The purpose of this study was to compare the distances covered during a 11-a-side soccer match after players had consumed either a high carbohydrate (CHO) or a low CHO diet. Twenty-two male professional soccer players formed 2 teams (A and B), of similar age, body characteristics, and training experience. The 2 teams played against each other twice with a week interval between.

Effect of cobalt(II) complexes with dipyridylamine and salicylaldehydes on cultured tumor and non-tumor cells: synthesis, crystal structure investigations and biological activity.

The synthesis of eight mixed-ligand cobalt(II) complexes with 2,2'-dipyridylamine (dpamH) and substituted salicylaldehydes (X-saloH) was undertaken in an effort to discover new compounds with anticancer activity. The complexes with the general formula [Co(dpamH)(2)(X-salo)]Y, (Y=Br or Cl) were characterized by elemental analyses, FT-IR and UV-visible spectroscopy, magnetic and conductivity measurements. The structures of two of them [Co(dpamH)(2)(5-CH(3)-salo)]Br and [Co(dpamH)(2)(3-OCH(3)-salo)]Cl, as well as of the precursors [Co(dpamH)(3)]Br(2) and [Co(dpamH)(2)Cl(H(2)O)]Cl, were determined by X-ray crystallography revealing octahedral coordination of cobalt(II) and mononuclear complexes.

(Carbonato-κO,O')bis-(di-2-pyridyl-amine-κN,N')cobalt(III) bromide.

In the title compound, [Co(CO(3))(C(10)H(9)N(3))(2)]Br, a distorted octa-hedral coordination of the Co(III) atom is completed by four N atoms of the two chelating di-2-pyridyl-amine ligands and two O atoms of the chelating carbonate anion. The di-2-pyridyl-amine ligands are nonplanar and the dihedral angles between the 2-pyridyl groups are 29.11 (9) and 37.

Efficacy of paracetamol versus diclofenac for Grade II ankle sprains.

Background: Ankle sprains are usually treated with the RICE protocol often with nonsteroidal anti-inflammatory drugs (NSAID) for pain reduction. We evaluated the effect of diclofenac, an NSAID, versus paracematol in the reduction of pain and acute edema of severe ankle sprains.

Materials And Methods: Ninety patients, 18 to 60 years old, with Grade II acute ankle sprains were randomized into two groups.

The effects of temperature on the physiological response to low oxygen in Atlantic sturgeon.

Atlantic sturgeon (Acipenser oxyrhynchus), which are bottom dwelling and migratory fish, experience environmental hypoxia in their natural environment. Atlantic sturgeon, acclimated to either 5 or 15°C, were subjected to a 1 h severe (<10 mm Hg) hypoxia challenge in order to document their physiological responses. We measured hematological parameters, including O(2) transport (hemoglobin, hematocrit), ionic (chloride, osmolality), and metabolic (glucose, lactate) variables under normoxic conditions (~160 mm Hg), immediately following a 1 h exposure to hypoxic water, and following a further 2 h of recovery from this challenge in normoxic water.

Optimization of cotton seed biodiesel quality (critical properties) through modification of its FAME composition by highly selective homogeneous hydrogenation.

The catalytic (homogeneous) hydrogenation of biodiesel's polyunsaturated fatty acid methyl esters (FAME), synthesized by transesterification of vegetable (cotton seed) oil, selectively to monounsaturated FAME, could upgrade the final quality of biodiesel. The final fuel can be optimized to have a higher cetane number and improved oxidative stability. The low-temperature performance after hydrogenation (CFPP) might be worst, but this, could be further improved through selective wintering and/or blending.

Transcription regulatory polymorphism -43T>C in the 5'-flanking region of SLC19A1 gene could affect rheumatoid arthritis patient response to methotrexate therapy.

The reduced folate carrier (RFC) protein (SLC19A1-gene) has central role in the uptake and intracellular accumulation of folates. In this respect, we investigate whether SLC19A1 genetic variations could affect rheumatoid arthritis (RA) patient response to antifolate treatment. One hundred six unrelated RA patients were enrolled in this study.