Infection with HIV can cripple the immune system and lead to AIDS. Hepatitis B virus (HBV) is a hepadnavirus that causes human liver diseases. Both pathogens are major public health problems affecting millions of people worldwide.
View Article and Find Full Text PDFDeoxythymidine triphosphate analogues with various 3' substituents in the sugar ring (-OH (dTTP)), -H, -N, -NH, -F, -O-CH, no group (2',3'-didehydro-2',3'-dideoxythymidine triphosphate (d4TTP)), and those retaining the 3'-OH but with 4' additions (4'--methyl, 4'--ethyl) or sugar ring modifications (d-carba dTTP) were evaluated using pre-steady-state kinetics in low (0.5 mM) and high (6 mM) Mg with HIV reverse transcriptase (RT). Analogues showed diminished observed incorporation rate constants ( ) compared to dTTP ranging from about 2-fold (3'-H, -N, and d4TTP with high Mg) to >10-fold (3'-NH and 3'-F with low Mg), while 3'-O-CH dTTP incorporated much slower than other analogues.
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