Follicular fluid and plasma lipidome profiling and associations towards embryonic development outcomes during ART treatment.

Introduction: It is well acknowledged that lipids assume a critical role in oocyte maturation and early embryonic metabolism, this study aimed to evaluate the relationship between the lipid composition of plasma and follicular fluid (FF), and the consequences of embryonic development. This study compared the lipidomic profiles of paired plasma and FF samples obtained from sixty-five Chinese women who underwent assisted reproductive technology (ART) treatments.

Methods: Non-targeted lipidomics analysis.

Multicomponent odd-parity superconductivity in UAu at high pressure.

We report that high-quality single crystals of the hexagonal heavy fermion material uranium diauride (UAu) become superconducting at pressures above 3.2 GPa, the pressure at which an unusual antiferromagnetic state is suppressed. The antiferromagnetic state hosts a marginal fermi liquid and the pressure evolution of the resistivity within this state is found to be very different from that approaching a standard quantum phase transition.

Erratum to "Mesenteric ectopic pregnancy with tubo-ovarian abscess" [Radiology Case Reports 16 (2021) 1165-1168].

[This corrects the article DOI: 10.1016/j.radcr.

Click-iT Plus OPP Alexa Fluor Protein Synthesis Assay in Embryonic Cells.

This protocol describes a method to assess relative changes in the level of global protein synthesis in the preimplantation embryo using the Click-iT Plus OPP Protein Synthesis Assays. In this assay, O-propargyl-puromycin (OPP), an analog of puromycin, is efficiently incorporated into the nascent polypeptide of newly translated proteins in embryonic cells. OPP is fluorescently labeled with a photostable Alexa Fluor dye and detected with fluorescence microscopy.

Congestive heart failure presenting as diffuse pulmonary nodules on CT.

Congestive heart failure (CHF) patients have typical radiological features that assist in diagnosis. This is a case that demonstrates an atypical radiologic presentation for CHF which showed diffuse pulmonary nodules on CT. Diffuse nodules can be present in several disease processes and clinical correlation is a necessity for diagnosis.

Non-Fermi liquid behavior below the Néel temperature in the frustrated heavy fermion magnet UAu.

The term Fermi liquid is almost synonymous with the metallic state. The association is known to break down at quantum critical points (QCPs), but these require precise values of tuning parameters, such as pressure and applied magnetic field, to exactly suppress a continuous phase transition temperature to the absolute zero. Three-dimensional non-Fermi liquid states, apart from superconductivity, that are unshackled from a QCP are much rarer and are not currently well understood.

Mechanical and Anatomical Alignment Guide Techniques Are Superior to Freehand in Achieving Target Orientation of an Acetabular Component.

Background: Achieving accurate and consistent acetabular component orientation remains a major challenge in total hip arthroplasty.

Methods: We used a pelvic model to compare freehand techniques vs mechanical and anatomical alignment guides in achieving a target operative inclination (OI) and operative anteversion (OA). Thirty subjects comprising consultant orthopedic surgeons, orthopedic trainees, and nonsurgical staff positioned an acetabular component in a pelvic model using 3 different methods for guiding inclination and another 3 for guiding version.

Field-Induced Modulated State in the Ferromagnet PrPtAl.

The theory of quantum order-by-disorder (QOBD) explains the formation of modulated magnetic states at the boundary between ferromagnetism and paramagnetism in zero field. PrPtAl has been argued to provide an archetype for this. Here, we report the phase diagram in magnetic field, applied along both the easy a axis and hard b axis.

Regulation of the mammalian maternal-to-embryonic transition by eukaryotic translation initiation factor 4E.

Eukaryotic translation initiation factor 4E (eIF4E) mediates cap-dependent translation. Genetic and inhibitor studies show that eIF4E expression is required for the successful transition from maternal to embryonic control of mouse embryo development. eIF4E was present in the oocyte and in the cytoplasm soon after fertilization and during each stage of early development.

Gastric gastrointestinal stromal tumor (GIST) with co-occurrence of pancreatic neuroendocrine tumor.

Mesenchymal tumors make up only about 1% of primary GI tumors, with Gastrointestinal Stromal Tumors (GIST) being the most common nonepithelial GI neoplasms. They are derived from the Interstitial cells of Cajal (ICC), and occur predominantly in older individuals, with a mean age of diagnosis of 64 years. Here we discuss the case of a 39-year-old female with atypical thoracic back pain wrapping around to the front and migrating diffuse abdomen pain that sometimes radiates into the chest.

Mesenteric ectopic pregnancy with tubo-ovarian abscess.

An ectopic pregnancy is the implantation of a fertilized ovum in a location other than the main cavity of the uterus. Ectopic pregnancies are reported in approximately 1%-2% of pregnancies, and while 95% of ectopic pregnancies are in fallopian tubes, only 3% are in ovarian, cervical, or abdominal sites. We present a case of a 38-year-old female with acute onset of severe lower abdominal pain, sepsis, chills, and diarrhea who was evaluated with a CT of the abdomen and pelvis with IV contrast only.

Composition dependence of the superconducting properties of UTe.

A better understanding of the synthesis conditions, composition and physical properties of UTeare required to interpret previously reported unconventional superconductivity. Here we report how the superconducting properties of single crystals depend on the ratio of elements present in their synthesis by chemical vapour transport. We have obtained crystals with the highest reported ambient pressureand a larger superconducting heat capacity jump from a growth with a U:Te ratio different from that widely used in the literature.

NAD Repletion Rescues Female Fertility during Reproductive Aging.

Reproductive aging in female mammals is an irreversible process associated with declining oocyte quality, which is the rate-limiting factor to fertility. Here, we show that this loss of oocyte quality with age accompanies declining levels of the prominent metabolic cofactor nicotinamide adenine dinucleotide (NAD). Treatment with the NAD metabolic precursor nicotinamide mononucleotide (NMN) rejuvenates oocyte quality in aged animals, leading to restoration in fertility, and this can be recapitulated by transgenic overexpression of the NAD-dependent deacylase SIRT2, though deletion of this enzyme does not impair oocyte quality.

Correction of pelvic adduction during total hip arthroplasty reduces variability in radiographic inclination: findings of a randomised controlled trial.

Introduction: The study aims were to identify the incidence of pelvic adduction during total hip arthroplasty (THA) in lateral decubitus and to determine, when aiming for 35° of apparent operative inclination (AOI), which of 3 operating table positions most accurately obtained a target radiographic inclination (RI) of 42°: (1) horizontal; (2) 7° head-down; (3) patient-specific position based on correction of pelvic adduction.

Methods: With patients seated on a levelled theatre table, a ruler incorporating a spirit level was used to draw transverse pelvic lines (TPLs) on the skin overlying the pelvis and sacrum. Subsequently, when positioned in lateral decubitus these lines provided a measure of pelvic adduction.

Reducing variability in apparent operative inclination during total hip arthroplasty: findings of a randomised controlled trial.

Aims: To determine which of 3 methods of cup insertion most accurately achieved a target apparent operative inclination (AOI) of 35° ± 2.5°: (1) Freehand; (2) Modified Mechanical Alignment Guide (MAG); or (3) Digital Inclinometer assisted.

Methods: Using a cementless cup via a posterior approach in lateral decubitus 270 participants were recruited, with 90 randomised to each method.

A prospective evaluation of a largely cementless total knee arthroplasty cohort without patellar resurfacing: 10-year outcomes and survivorship.

Background: The theoretical benefits of a mobile bearing design in Total Knee Arthroplasty (TKA) include increased articular surface conformity with a reduction in both polyethylene wear and implant interface shear. However, to date these theoretical advantages have not been translated into published evidence of superior survivorship. This paper presents the results of a prospective, non-comparative study evaluating the performance of the mobile bearing Low Contact Stress LCS Complete Rotating Platform TKA in a largely cementless cohort without patellar resurfacing.

Immunological Staining of Global Changes in DNA Methylation in the Early Mammalian Embryo.

The structural complexity of chromatin can make antibody access to some nuclear antigens energetically unfavorable. This can limit the usefulness and reliability of immunostaining unless validated methods of epitope retrieval are applied. We found that denaturation of chromatin by sequential use of acidification and tryptic digestion of fixed cells is required to reliably detect DNA methylation in the embryo.

Differences in the nutrient concentrations, in vitro methanogenic potential and other fermentative traits of tropical grasses and legumes for beef production systems in northern Australia.

Background: In northern Australia, beef cattle grazed extensively on tropical rangelands are responsible for 5% of the nation's total greenhouse gas emissions. Methane (CH ) is a potent greenhouse gas and in grazing ruminants might be mitigated by selecting forages that, when consumed, produce less CH when fermented by rumen microbes. This study examined variability in the in vitro fermentation patterns, including CH production of selected tropical grasses and legumes, to identify candidates for CH mitigation in grazing livestock in northern Australia.

Iatrogenic Radial Nerve Palsy following Closed Reduction of a Simple Diaphyseal Humeral Fracture: Beware the Perfect X-Ray.

Radial nerve injury is a recognised complication associated with humeral shaft fracture. A case of iatrogenic radial nerve injury is presented following fracture reduction. The relevant anatomy, challenges in management of humeral fractures with associated radial nerve injury, and the importance of detailed clinical assessment and documentation are discussed.

Acetabular Liner Dissociation following Total Hip Arthroplasty: A Rare but Serious Complication That May Be Easily Misinterpreted in the Emergency Department.

Acetabular liner dissociation is a rare complication of Total Hip Arthroplasty (THA) which requires urgent revision surgery. A case is presented in which the correct diagnosis was not appreciated on two separate Emergency Department attendances. The typical symptoms, signs, and radiological features are outlined and the importance of considering a rare complication following a commonly performed procedure is highlighted.

Impacts of and interactions between environmental stress and epigenetic programming during early embryo development.

The processes of assisted reproductive technologies (ART) involve a variety of interventions that impact on the oocyte and embryo. Critically, these interventions cause considerable stress and coincide with important imprinting events throughout gametogenesis, fertilisation and early embryonic development. It is now accepted that the IVM and in vitro development of gametes and embryos can perturb the natural course of development to varying degrees of severity.

The epigenetic bivalency of core pancreatic β-cell transcription factor genes within mouse pluripotent embryonic stem cells is not affected by knockdown of the polycomb repressive complex 2, SUZ12.

This study assesses changes in activator and repressor modifications to histones associated with the core transcription factor genes most highly upregulated or downregulated in pancreatic β-cells relative to expression in an embryonic stem cell line. Epigenetic analysis of the Oct4, Utf1, Nanog and Sox2 (pluripotency) and Pdx1, Nkx6.1, Nkx2.