Publications by authors named "Christopher L Johnny"
Investigating cholesterol trafficking pathways continues to be of significant scientific interest owing to its homeostasis being associated with several debilitating cardiovascular and neurodegenerative diseases including atherosclerosis, Niemann-Pick's disease, Alzheimer's disease, and Parkinson's disease. To further our understanding of cholesterol trafficking, it is imperative to develop new fluorescent probes that possess improved photostability, low efflux, and high spatial and temporal resolution for live-cell imaging. In this study, we developed a photoconvertible fluorescent cholesterol analog, Duo-Chol, enabling the improved spatiotemporal fluorescence imaging of the dynamic localization of cholesterol in live cells.
View Article and Find Full Text PDFWithin the past two decades, photoconvertible fluorescent proteins (PC-FPs) have emerged as a class of useful proteins for the visualization and tracking of individual cells, complex cellular mechanisms, protein-protein interactions, and other dynamic processes. Despite the utility of these proteins, they are inherently limited by a number of factors including large size and inflexibility of tag location within a protein of interest. The following chapter describes the discovery and use of a small molecule photoconvertible dye based on the novel diazaxanthilidene scaffold.
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- Cycloheximide (CHX) is a widely used inhibitor of protein synthesis in eukaryotic cells, but research on its synthetic derivatives has been limited.
- The article outlines the total synthesis of CHX and its analogues, examining the structure-activity relationships (SAR) that influence their ability to inhibit translation.
- The SAR studies led to the creation of more effective compounds, including one that irreversibly inhibits ribosomal elongation, potentially enhancing research into protein synthesis.