Molecular regulation of virulence in Legionella pneumophila.

Legionella pneumophila is a gram-negative bacteria found in natural and anthropogenic aquatic environments such as evaporative cooling towers, where it reproduces as an intracellular parasite of cohabiting protozoa. If L. pneumophila is aerosolized and inhaled by a susceptible person, bacteria may colonize their alveolar macrophages causing the opportunistic pneumonia Legionnaires' disease.

Autorepressor PsrA is required for optimal Legionella pneumophila growth in Acanthamoeba castellanii protozoa.

Legionella pneumophila possesses a unique intracellular lifecycle featuring distinct morphological stages that include replicative forms and transmissive cyst forms. Expression of genes associated with virulence traits and cyst morphogenesis is concomitant, and governed by a complex stringent response based-regulatory network and the stationary phase sigma factor RpoS. In Pseudomonas spp.

Development of a Fluorescent Tool for Studying Intracellular Infection.

Legionnaires' disease incidence is on the rise, with the majority of cases attributed to the intracellular pathogen, Nominally a parasite of protozoa, can also infect alveolar macrophages when bacteria-laden aerosols enter the lungs of immunocompromised individuals. pathogenesis has been well characterized; however, little is known about the >25 different spp. that can cause disease in humans.

Complete Genome Sequences of Two Environmental Isolates Obtained from Potable Water Sourced in a First Nation Community.

Here, we report the complete genome sequences of two distinct isolates of that were obtained from potable water sourced from cistern-bearing homes within a First Nation community in Manitoba, Canada.

A response regulator protein with antar domain, AvnR, in ATCC 17978 impacts its virulence and amino acid metabolism.

a Gram-negative coccobacillus, is notorious for its involvement in opportunistic infections around the world. Its resistance to antibiotics makes treatment of infections challenging. In this study, we describe a novel response regulator protein, AvnR (A1S_2006) that regulates virulence-related traits in ATCC17978.

Examining protein protein interactions using endogenously tagged yeast arrays: the cross-and-capture system.

Comprehensive approaches to detect protein-protein interactions (PPIs) have been most successful in the yeast model system. Here we present "Cross-and-Capture," a novel assay for rapid, sensitive assessment of PPIs via pulldown of differently tagged yeast strain arrays. About 500 yeast genes that function in DNA replication, repair, and recombination and nuclear proteins of unknown function were chromosomally tagged with six histidine residues or triple VSV epitopes.