Selective B cell depletion upon intravenous infusion of replication-incompetent anti-CD19 CAR lentivirus.

Anti-CD19 chimeric antigen receptor (CAR)-T therapy for B cell malignancies has shown clinical success, but a major limitation is the logistical complexity and high cost of manufacturing autologous cell products. If engineered for improved safety, direct infusion of viral gene transfer vectors to initiate CAR-T transduction, expansion, and anti-tumor activity could provide an alternative, universal approach. To explore this approach we administered approximately 20 million replication-incompetent vesicular stomatitis virus G protein (VSV-G) lentiviral particles carrying an anti-CD19CAR-2A-GFP transgene comprising either an FMC63 (human) or 1D3 (murine) anti-CD19 binding domain, or a GFP-only control transgene, to wild-type C57BL/6 mice by tail vein infusion.

Opposing Effects of Valproic Acid Treatment Mediated by Histone Deacetylase Inhibitor Activity in Four Transgenic Models of Retinitis Pigmentosa.

Retinitis pigmentosa (RP) is an inherited retinal degeneration (RD) that leads to blindness for which no treatment is available. RP is frequently caused by mutations in ; in some animal models, RD is exacerbated by light. Valproic acid (VPA) is a proposed treatment for RP and other neurodegenerative disorders, with a phase II trial for RP under way.

Spiritual life after cancer: connectedness and the will to meaning as an expression of self-help.

This article uses a mixed quantitative/qualitative design to elicit the attitudes and experience of spirituality in a group of N = 11 heterogeneous cancer participants, who were interviewed regarding self-help practice. Part of this interview enquired about spirituality and was analysed separately from the larger body of data, becoming the Spiritual Interview. The authors argue for a conceptualisation of spirituality as a "will to meaning" and "connectedness.