Command line to pipeLine: Cross-biobank analyses with Nextflow.

Biobanks hold immense potential for genomic research, but fragmented data and incompatible tools slow progress. This workshop equipped participants with Nextflow, a powerful workflow language to streamline bioinformatic analyses across biobanks. We taught participants to write code in their preferred language and demonstrated how Nextflow handles the complexities, ensuring consistent, reproducible results across different platforms.

Clinical correlates of CT imaging-derived phenotypes among lean and overweight patients with hepatic steatosis.

The objective of this study is to define CT imaging derived phenotypes for patients with hepatic steatosis, a common metabolic liver condition, and determine its association with patient data from a medical biobank. There is a need to further characterize hepatic steatosis in lean patients, as its epidemiology may differ from that in overweight patients. A deep learning method determined the spleen-hepatic attenuation difference (SHAD) in Hounsfield Units (HU) on abdominal CT scans as a quantitative measure of hepatic steatosis.

Magneto-optical trapping in a near-suface borehole.

Borehole gravity sensing can be used in a number of applications to measure features around a well, including rock-type change mapping and determination of reservoir porosity. Quantum technology gravity sensors, based on atom interferometry, have the ability to offer increased survey speeds and reduced need for calibration. While surface sensors have been demonstrated in real world environments, significant improvements in robustness and reductions to radial size, weight, and power consumption are required for such devices to be deployed in boreholes.

1,4-Diamination of cyclic dienes via a (4 + 3) cycloaddition of diaza-allyl cationic intermediates.

Diaza-(4 + 3) cycloadditions of putative diaza-oxyallyl cationic intermediates and cyclic dienes are reported as a method for the 1,4-diamination of cyclic dienes. This reaction was entirely selective for diamination and provided cycloadducts in good to excellent yield.

Generation and reactivity of aza-oxyallyl cationic intermediates: aza-[4 + 3] cycloaddition reactions for heterocycle synthesis.

Aza-[4 + 3] cycloadditions of putative aza-oxyallyl cationic intermediates and cyclic dienes are reported. The intermediate is generated by the dehydrohalogenation of α-haloamides. The reaction is general to a variety of α-haloamides and is diastereoselective.

Comparison of magnetic resonance feature tracking for strain calculation with harmonic phase imaging analysis.

Objectives: To compare a steady-state free precession cine sequence-based technique (feature tracking [FT]) to tagged harmonic phase (HARP) analysis for peak average circumferential myocardial strain (epsilon(cc)) analysis in a large and heterogeneous population of boys with Duchenne muscular dystrophy (DMD).

Background: Current epsilon(cc) assessment techniques require cardiac magnetic resonance-tagged imaging sequences, and their analysis is complex. The FT method can readily be performed on standard cine (steady-state free precession) sequences.

KCNQ currents and their contribution to resting membrane potential and the excitability of interstitial cells of Cajal from the guinea pig bladder.

Purpose: The presence of novel KCNQ currents was investigated in guinea pig bladder interstitial cells of Cajal and their contribution to the maintenance of the resting membrane potential was assessed.

Materials And Methods: Enzymatically dispersed interstitial cells of Cajal were patch clamped with K(+) filled pipettes in voltage clamp and current clamp modes. Pharmacological modulators of KCNQ channels were tested on membrane currents and the resting membrane potential.

Survey response inducements for registered nurses.

The past 20 years have seen an overall decline in survey response rates and an even more pronounced decline in samples of health care professionals. The authors tested the use of a "thank you" or "reminder" postcard as a method by which to stem the tide of declining response rates. The authors conducted a mail and telephone survey of 49,605 registered nurses for the 2000 National Sample Survey of Registered Nurses and sent an extra mailing to a random subsample (n = 4,968).