Refined methodology for quantifying virulence using .

Larvae of (the greater wax moth) are being increasingly used as a model to study microbial pathogenesis. In this model, bacterial virulence is typically measured by determining the 50% lethal dose (LD) of a bacterial strain or mutant. The use of to study pathogenesis, however, is challenging because of the extreme sensitivity of larvae to this bacterium.

Klebsiella pneumoniae clinical isolates with features of both multidrug-resistance and hypervirulence have unexpectedly low virulence.

Klebsiella pneumoniae has been classified into two types, classical K. pneumoniae (cKP) and hypervirulent K. pneumoniae (hvKP).

Genome-wide screens reveal shared and strain-specific genes that facilitate enteric colonization by .

is a common cause of difficult-to-treat infections due to its propensity to express resistance to many antibiotics. For example, carbapenem-resistant has been named an urgent threat by the United States Centers for Disease Control and Prevention. Gastrointestinal colonization in patients with has been linked to subsequent infection, making it a key process to control in the prevention of multidrug-resistant infections.

Genome-wide screens reveal shared and strain-specific genes that facilitate enteric colonization by .

Unlabelled: Gastrointestinal (GI) colonization by is a risk factor for subsequent infection as well as transmission to other patients. Additionally, colonization is achieved by many strain types that exhibit high diversity in genetic content. Thus, we aimed to study strain-specific requirements for GI colonization by applying transposon insertion sequencing to three classical clinical strains: a carbapenem-resistant strain, an extended-spectrum beta-lactamase producing strain, and a non-epidemic antibiotic-susceptible strain.

Deterministic teleportation of a quantum gate between two logical qubits.

A quantum computer has the potential to efficiently solve problems that are intractable for classical computers. However, constructing a large-scale quantum processor is challenging because of the errors and noise that are inherent in real-world quantum systems. One approach to addressing this challenge is to utilize modularity-a strategy used frequently in nature and engineering to build complex systems robustly.

A Schrödinger cat living in two boxes.

Quantum superpositions of distinct coherent states in a single-mode harmonic oscillator, known as "cat states," have been an elegant demonstration of Schrödinger's famous cat paradox. Here, we realize a two-mode cat state of electromagnetic fields in two microwave cavities bridged by a superconducting artificial atom, which can also be viewed as an entangled pair of single-cavity cat states. We present full quantum state tomography of this complex cat state over a Hilbert space exceeding 100 dimensions via quantum nondemolition measurements of the joint photon number parity.

High-speed discrimination and sorting of submicron particles using a microfluidic device.

The size- and fluorescence-based sorting of micro- and nanoscale particles suspended in fluid presents a significant and important challenge for both sample analysis and for manufacturing of nanoparticle-based products. Here, we demonstrate a disposable microfluidic particle sorter that enables high-throughput, on-demand counting and binary sorting of submicron particles and cells using either fluorescence or an electrically based determination of particle size. Size-based sorting uses a resistive pulse sensor integrated on-chip, whereas fluorescence-based discrimination is achieved using on-the-fly optical image capture and analysis.