Inhibition of Pyruvate Kinase From by IMP Is Independent of the Extra-C Domain.

The pyruvate kinase (PYK) isozyme from (TsPYK) has previously been used in metabolic engineering for improved ethanol production. This isozyme belongs to a subclass of PYK isozymes that include an extra C-domain. Like other isozymes that include this extra C-domain, we found that TsPYK is activated by AMP and ribose-5-phosphate (R5P).