Collagen crosslinking-induced corneal morphological changes: a three-dimensional light sheet Microscopy-based evaluation.

Stiffness-related eye diseases such as keratoconus require comprehensive visualization of the complex morphological matrix changes. The aim of this study was to use three-dimensional (3D) light sheet fluorescence microscopy (LSFM) to analyze unlabeled corneal tissue samples, qualitatively visualizing changes in corneal stiffness. Isolated porcine corneal tissue samples were treated with either NaCl or 0.

Digitalomics - digital transformation leading to omics insights.

Introduction: Biomarker discovery is increasingly moving from single omics to multiomics, as well as from multi-cell omics to single-cell omics. These transitions have increasingly adopted digital transformation technologies to accelerate the progression from data to insight. Here, we will discuss the concept of 'digitalomics' and how digital transformation directly impacts biomarker discovery.

Ultrabroadband two-beam coherent anti-Stokes Raman scattering and spontaneous Raman spectroscopy of organic fluids: A comparative study.

Spontaneous Raman spectroscopy is a well-established diagnostic tool, allowing for the identification of all Raman active species with a single measurement. Yet, it may suffer from low-signal intensity and fluorescent background. In contrast, coherent anti-Stokes Raman scattering (CARS) offers laser-like signals, but the traditional approach lacks the multiplex capability of spontaneous Raman spectroscopy.

Spatial correlation of 2D hard-tissue histology with 3D microCT scans through 3D printed phantoms.

Hard-tissue histology-the analysis of thin two-dimensional (2D) sections-is hampered by the opaque nature of most biological specimens, especially bone. Therefore, the cutting process cannot be assigned to regions of interest. In addition, the applied cutting-grinding method is characterized by significant material loss.

Pre-hyperglycemia immune cell trafficking underlies subclinical diabetic cataractogenesis.

Background: This work elucidates the first cellular and molecular causes of cataractogenesis. Current paradigm presupposes elevated blood glucose as a prerequisite in diabetic cataractogenesis. Novel evidence in our model of diabetic cataract challenges this notion and introduces immune cell migration to the lens and epithelial-mesenchymal transformation (EMT) of lens epithelial cells (LECs) as underlying causes.

Current Approaches for Image Fusion of Histological Data with Computed Tomography and Magnetic Resonance Imaging.

Classical analysis of biological samples requires the destruction of the tissue's integrity by cutting or grinding it down to thin slices for (Immuno)-histochemical staining and microscopic analysis. Despite high specificity, encoded in the stained 2D section of the whole tissue, the structural information, especially 3D information, is limited. Computed tomography (CT) or magnetic resonance imaging (MRI) scans performed prior to sectioning in combination with image registration algorithms provide an opportunity to regain access to morphological characteristics as well as to relate histological findings to the 3D structure of the local tissue environment.

[Association, expectations and barriers of the use of exoskeletons in small and medium-sized enterprises].

Background: In the manufacturing industry, work-related musculoskeletal disorders (MSD) result in sick days and have substantial economic consequences for the enterprise and the national economy. Exoskeletons can support the body when handling heavy loads and enduring enforced postures. Exoskeletons are being piloted particularly in large companies in the automotive industry; however, exoskeletons have so far attracted little interest in small and medium-sized enterprises (SME) and their use has so far barely been scientifically examined.

Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry.

Transcription factors (TFs) regulate target genes by specific interactions with DNA sequences. Detecting and understanding these interactions at the molecular level is of fundamental importance in biological and clinical contexts. Crosslinking mass spectrometry is a powerful tool to assist the structure prediction of protein complexes but has been limited to the study of protein-protein and protein-RNA interactions.

Lipid Emulsion-Based OCT Angiography for Ex Vivo Imaging of the Aqueous Outflow Tract.

Purpose: Contrast agents applicable for optical coherence tomography (OCT) imaging are rare. The intrascleral aqueous drainage system would be a potential application for a contrast agent, because the aqueous veins are of small diameter and located deep inside the highly scattering sclera. We tested lipid emulsions (LEs) as candidate OCT contrast agents in vitro and ex vivo, including milk and the anesthetic substance Propofol.

Automated Retinal Image Analysis for Evaluation of Focal Hyperpigmentary Changes in Intermediate Age-Related Macular Degeneration.

Purpose: To develop and evaluate a software tool for automated detection of focal hyperpigmentary changes (FHC) in eyes with intermediate age-related macular degeneration (AMD).

Methods: Color fundus (CFP) and autofluorescence (AF) photographs of 33 eyes with FHC of 28 AMD patients (mean age 71 years) from the prospective longitudinal natural history MODIAMD-study were included. Fully automated to semiautomated registration of baseline to corresponding follow-up images was evaluated.

In vivo imaging with a fundus camera in a rat model of laser-induced choroidal neovascularization.

Purpose: To investigate the use of imaging and quantitative measurement capabilities of a modified fundus camera in a rat model of laser-induced choroidal neovascularization.

Methods: Following induction of experimental choroidal neovascularization, Dark Agouti rats underwent serial in vivo imaging with a fundus camera (FF450plus, Carl Zeiss MediTec, Jena, Germany), including color, reflectance and fluorescence imaging.

Results: A custom-made setting allowed high-resolution imaging.

Vector analysis of myopic astigmatism corrected by femtosecond refractive lenticule extraction.

Purpose: To evaluate the visual outcome of refractive lenticule extraction in eyes with myopic astigmatism using the Visumax femtosecond laser.

Setting: Department of Ophthalmology, HELIOS Klinikum Erfurt, Erfurt, Germany.

Design: Nonrandomized clinical trials.

Effect of a suction device for femtosecond laser on anterior chamber depth and crystalline lens position measured by OCT.

Purpose: To investigate the position and stability of the crystalline lens after application of a suction device containing a contact lens and a vacuum unit for the treatment of presbyopia using a femtosecond laser.

Methods: Twenty presbyopic (44.4+/-4.

First efficacy and safety study of femtosecond lenticule extraction for the correction of myopia: six-month results.

Purpose: To prospectively study the feasibility of femtosecond lenticule extraction (FLE), a new method of refractive correction.

Setting: Department of Ophthalmology, Philipps University of Marburg and Helios Clinic, Erfurt, Germany.

Methods: A flap and a lenticule of intrastromal corneal tissue were simultaneously cut with a VisuMax femtosecond laser system.