Clinical Applicability of Tissue Polypeptide Antigen and CA-125 in Gynecological Malignancies.

Background: Nowadays there still is no sufficient screening tool for ovarian and uterine cancer.

Objective: The current study aimed to investigate whether cancer antigen 125 (CA-125), tissue polypeptide antigen (TPA) or the combination of both markers are able to act as screening tools for ovarian or uterine cancer.

Methods: A total of 275 blood samples from different cohorts (ovarian cancer, uterine cancer, benign control group) were prospectively drawn and analyzed.

Evaluation of the New Cyclosporine and Tacrolimus Automated Electrochemiluminescence Immunoassays under Field Conditions.

Background: Careful monitoring of the post-transplantation immunosuppressant drugs (ISDs) cyclosporine (CsA) and tacrolimus (TAC) in whole blood is essential to prevent adverse drug events. Immunoassays represent the most widely used methodology for therapeutic drug monitoring. In this study, the technical performance of the new automated electrochemiluminescence immunoassays (ECLIAs) for CsA and TAC measurement were assessed under field conditions.

Accuracy and clinical implications of seven 25-hydroxyvitamin D methods compared with liquid chromatography-tandem mass spectrometry as a reference.

Background: The most reliable assessment of vitamin D status is measurement of plasma 25-hydroxyvitamin D (25[OH]D) concentration. High variability in 25(OH)D measurements due to utilized test and assay technologies and the lack of standardization against reference materials and reference method often confounds proper assessment of vitamin D status.

Methods: We evaluated the accuracy of six routinely available methodologies: high-performance liquid chromatography (HPLC), the IDS-radioimmunoassay (IDS-RIA) and enzyme immunoassay (IDS-EIA), the Nichols Advantage automated protein-binding assay (Advantage), two versions of the DiaSorin automated immunoassay (Liaison 1 and Liaison 2)--and one prototype automated immunoassay (Elecsys) for assessment of the 25(OH)D(3) status in a cohort of 300 randomly selected patients' samples compared with the reference method liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Quantitative bedside testing of troponin T: is it equal to laboratory testing? The Cardiac Reader Troponin T (CARE T) study.

The progressive evolution of cardiac marker testing in patients with acute coronary syndromes has extended their role into risk stratification and guidance of therapeutic regimen. To provide utilization of cardiac markers around the clock and facilitate the diagnostic work-up of patients with acute chest pain in the emergency room, a point-of-care system for quantitative troponin T and myoglobin testing in whole blood samples was developed. Aim of this multicenter study was to evaluate bedside quantitative determination of myoglobin and troponin T in chest pain patients in a clinical routine setting.