Publications by authors named "Christoph J Hueck"
Article Synopsis
- A new method called insertion-duplication mutagenesis (IDM) was developed to identify essential genes in Salmonella enterica serovar Typhimurium by using small genomic fragments and a conditionally replicating vector.
- The research involved growing a library of Salmonella clones under different temperature conditions, allowing scientists to distinguish between harmful and harmless genetic insertions to locate essential genes.
- Out of 498 fragments tested that led to lethal knockouts, 145 were known essential genes and 112 were novel candidates, highlighting that around 490 genes appear to be critical for growth in nutrient-rich environments.
