Enzyme cascade converting cyclohexanol into ε-caprolactone coupled with NADPH recycling using surface displayed alcohol dehydrogenase and cyclohexanone monooxygenase on E. coli.

The application of enzymes as biocatalysts in industrial processes has great potential due to their outstanding stereo-, regio- and chemoselectivity. Using autodisplay, enzymes can be immobilized on the cell surface of Gram-negative bacteria such as Escherichia coli. In the present study, the surface display of an alcohol dehydrogenase (ADH) and a cyclohexanone monooxygenase (CHMO) on E.

Cell density-dependent auto-inducible promoters for expression of recombinant proteins in Pseudomonas putida.

Inducible promoters such as P are of limited usability for industrial protein production with Pseudomonas putida. We therefore utilized cell density-dependent auto-inducible promoters for recombinant gene expression in P. putida KT2440 based on the RoxS/RoxR Quorum Sensing (QS) system of the bacterium.

Direct optical density determination of bacterial cultures in microplates for high-throughput screening applications.

A convenient and most abundantly applied method to determine the growth state of a bacterial cell culture is to determine the optical density (OD) spectrophotometrically. Dilution of the samples, which is necessary to measure within the linear range of the spectrophotometer, is time-consuming and not compatible with high-throughput applications. Here we present a direct approach to estimate the OD at 578 nm (OD) of bacterial cultures in microplates without the need for sample dilution.