Strategy for analysis of cardiac troponins in biological samples with a combination of affinity chromatography and mass spectrometry.

Background: Cardiac troponins are modified during ischemic injury and are found as a heterogeneous mixture in blood of patients with cardiovascular diseases. We present a strategy to isolate cardiac troponins from human biological material, by use of affinity chromatography, and to provide samples ready for direct analysis by mass spectrometry.

Methods: Cardiac troponins were isolated from human left ventricular tissue by affinity chromatography.