A glucose oxidase peroxidase-coupled continuous assay protocol for the determination of cellulase activity in the laboratory: the Abuajah method.

This present study reports a novel laboratory protocol, the glucose oxidase peroxidase (GOPOD)-coupled continuous assay (GcCA) which is based on the general principle that all cellulases generate β-d-glucose as one of their hydrolytic products. Resultantly, the β-d-glucose generated serves as a substrate to an auxiliary enzyme, GOPOD, coupled to the cellulase hydrolytic process, in a simultaneous secondary reaction which continuously quantifies the concentration of glucose generated as a measure of incremental cellulase activity in the laboratory. The protocol is initial hydrolytic rate-driven.