De novo genome assembly and comparative genomics for the colonial ascidian Botrylloides violaceus.

Ascidians have the potential to reveal fundamental biological insights related to coloniality, regeneration, immune function, and the evolution of these traits. This study implements a hybrid assembly technique to produce a genome assembly and annotation for the botryllid ascidian, Botrylloides violaceus. A hybrid genome assembly was produced using Illumina, Inc.

Sex Attractant Pheromones of Virgin Queens of Sympatric Slave-Making Ant Species in the Genus Polyergus, and their Possible Roles in Reproductive Isolation.

Species of the ant genus Polyergus are social parasites that steal brood from colonies of their hosts in the closely related genus Formica. Upon emergence as adults in a mixed population, host Formica workers carry out all the normal worker functions within the Polyergus colony, including foraging, feeding, grooming, and rearing brood of the parasitic Polyergus ants. Some unmated Polyergus gynes (queens) run in the raiding columns of their colonies and attract males by releasing a pheromone from their mandibular glands.

Attitudes Toward Divorce, Commitment, and Divorce Proneness in First Marriages and Remarriages.

A random multistate sample of married individuals ( = 1,931) was used to explore whether more positive attitudes toward divorce and weaker commitment to marriage may contribute to the greater instability of remarriages than first marriages. Remarried adults, whether or not they brought children from a previous union into the remarriage, reported marital quality (happiness and conflict) equal to those in first marriages. They also reported more positive attitudes toward divorce, which were associated with higher divorce proneness (i.

The Timing of Cohabitation and Engagement: Impact on First and Second Marriages.

Using a multi-state sample of marriages that took place in the 1990s, this study examined associations between premarital cohabitation history and marital quality in first (N = 437) and second marriages (N = 200), and marital instability in first marriages (intact N = 521, divorced N = 124). For first marriages, cohabiting with the spouse without first being engaged or married was associated with more negative interaction, higher self-reported divorce proneness, and a greater probability of divorce compared to cohabiting after engagement or marriage (with patterns in the same direction for marital positivity). In contrast, there was a general risk associated with premarital cohabitation for second marriages on self-reported indices of marital quality, with or without engagement when cohabitation began.

Differential use of premarital education in first and second marriages.

Although second marriages are more likely to end in divorce than first marriages, and thus represent an important target for intervention, there have been no detailed examinations of the use of premarital education in second marriages. Using random-digit dialing methods, 398 individuals currently in a second marriage and 1,342 individuals currently in a first marriage participated. Compared with those in first marriages, individuals in second marriages were significantly less likely to receive premarital education for their current marriage.

Impact of parasite sympatry on the geographic mosaic of coevolution.

Slave-making ants are specialized social parasites that steal the young from colonies of their host species to augment their slave supply. The degree of parasite-host specialization has been shown to shape the trajectory along which parasites and hosts coevolve and is a prime contributor to the geographic mosaic of coevolution. However, virtually nothing is known about extrinsic influences on parasite-host dynamics, although the simple addition of a competing slave-maker may significantly alter selection pressures.

Premarital education, marital quality, and marital stability: findings from a large, random household survey.

One of the limitations of experimental studies on the effectiveness of premarital education is the reliance on samples of mostly White, middle-class couples. In contrast, although survey methods allow only weak inferences about causal relations, representative surveys can yield important information about use and estimated effects across a diverse population. Using a large random survey of 4 middle American states, the authors found that participation in premarital education was associated with higher levels of satisfaction and commitment in marriage and lower levels of conflict-and also reduced odds of divorce.

Recruitment of healthy participants for studies on risks for alcoholism: effectiveness of random digit dialling.

Aims: To compare the effectiveness of two strategies for recruiting healthy research volunteers.

Methods: Demographic characteristics and recruitment costs of participants who completed a laboratory study examining risk factors for alcoholism recruited through random digit dialling (N = 11) and community advertisements (N = 102) were compared.

Results: Advertisement yielded a more representative sample [76% Caucasian, less well educated (M = 15.

A diabetes prevention assessment tool for American Indians.

Introduction: American Indians have a disproportionately higher risk of developing type 2 diabetes. Few data are available about the perceptions of diabetes among American Indians, and no culturally appropriate tools are available for assessment of perceptions related to health and diabetes.

Methods: A diabetes prevention assessment tool was developed to measure perceptions of health and diabetes among American Indians.

Patterns of continuing professional education in registered dietitians and dietetic technicians, registered.

This study compared the continuing professional education (CPE) activities of registered dietitians (RDs) with those of dietetic technicians, registered (DTRs). A descriptive research study was used with data from the baseline survey evaluating the Commission on Dietetic Registration's (CDR) Professional Development Portfolio. The survey was sent to 3,530 randomly selected RDs and DTRs; 1,429 surveys were returned (40.

Response of Diamond-Blackfan anemia to metoclopramide: evidence for a role for prolactin in erythropoiesis.

A 47-year-old woman with severe macrocytic anemia markedly improved during the second and third trimesters of 3 pregnancies and when breast-feeding her 2 children. Because the serum prolactin level is elevated at these times, we later treated her with metoclopramide (10 mg orally 3 times daily), a medication known to induce prolactin release. Her serum prolactin levels increased from 7 to 133 ng/mL (normal < 20 ng/mL) and hematocrit from 17% to 22% to 35%.

Interpretation of fetal hemoglobin only on newborn screening for hemoglobinopathy.

Newborn screening for hemoglobinopathies rarely produces a fetal hemoglobin only result; it is most consistent with beta-thalassemia major, although other diagnoses are possible. The authors describe two unrelated African-American babies born in North Carolina whose newborn screening revealed fetal hemoglobin only. Both had a relatively benign clinical and hematologic picture.