Publications by authors named "Christina V Dinh"
Article Synopsis
- Metabolic engineering involves modifying cells to produce valuable compounds by altering or introducing genes for specific enzymatic reactions.
- Dynamic regulation of metabolic processes helps manage the competing demands on cells caused by these engineered pathways.
- The review discusses strategies for dynamic regulation, including how responsive control circuits are designed to optimize shifts in metabolic activity based on specific challenges.
Layered and multi-input autonomous dynamic control strategies for metabolic engineering.
Curr Opin Biotechnol
October 2020
Article Synopsis
- Metabolic engineering aims to redesign cells to produce valuable chemicals more efficiently through methods like overexpressing pathways and knocking out competing ones.
- Dynamic control of metabolic flux can enhance this process by adjusting cell metabolism in real-time based on fermentation conditions, enabling more effective production.
- Recent studies highlight innovative strategies, including multiplexed autonomous systems that manage multiple regulatory circuits and respond to various signals, potentially solving complex metabolic challenges.
Article Synopsis
- Advances in synthetic biology and metabolic engineering enable the creation of complex microbial synthesis systems, but individual cells often face limitations in machinery and resources.
- Coculture fermentation offers a solution by allowing different microbial populations to share production tasks, though traditional methods mainly focus on adjusting initial cell mixes.
- A new growth-regulation circuit based on quorum sensing significantly improved naringenin production, achieving a 60% increase in yield, and when combined with a communication module, led to an additional 60% boost in production efficiency.
Development of an autonomous and bifunctional quorum-sensing circuit for metabolic flux control in engineered .
Proc Natl Acad Sci U S A
December 2019
Article Synopsis
- Metabolic engineering aims to optimize microbial cells for the sustainable production of valuable compounds, balancing natural cellular processes with production goals.
- Researchers have introduced dynamic regulation techniques that adjust gene expression based on cell density, enabling more efficient metabolic flux toward product formation.
- A newly developed quorum-sensing regulation tool allows simultaneous control of multiple gene sets, demonstrating effective tuning and application in synthesizing naringenin and salicylic acid, thus confirming its utility in complex pathways.
Article Synopsis
- The main challenge in cancer gene therapy is finding effective delivery methods that protect the genes from degradation and minimize harmful side effects.
- PEGylated liposomes have shown promise in delivering small molecule drugs and may be effective for nucleic acid delivery, thanks to their PEG coating which helps avoid immune response and enhance circulation.
- This study focused on using PEGylated liposomes to deliver small interfering RNA (siRNA) for silencing genes associated with human papillomavirus (HPV) and cervical cancer, emphasizing the importance of targeting peptides and polymer complexation for successful delivery.