Publications by authors named "Christina Pitsillidou"
Flow cytometry enables the identification and characterization of markers present on the cell membrane as well as those that manifest intracellularly. With the increasing number of available reagents for targeting the markers of interest and evolving technology, it has become possible to detect an increasing number of markers expressed by single cells during one single analysis. This provides the possibility of investigating cell-to-cell patterns, variations, and correlations.
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- The study introduces a flow cytometry assay that allows for the simultaneous measurement of antioxidant defense and reactive oxygen species in different immune cell types, including T, B, and natural killer lymphocytes.
- It outlines the procedures for preparing peripheral blood mononuclear cells, including staining and fixation methods necessary for accurate analysis.
- The paper also details the standardization of the machine used, as well as guidelines for data acquisition and analysis.