Bosea rubneri sp. nov. Isolated from Organically Grown Allium cepa.

Strain ZW T0_25 was isolated from an onion sample (Allium cepa var. Hytech F1) within a storage trial and proofed to be a novel, aerobic, Gram-stain negative, rod-shaped bacterial strain. Analyses of the 16S rRNA gene sequence and of the whole draft genome sequences, i.

sp. nov. and sp. nov., originating from chilled beef and chicken breast.

By investigating wet and dry age-related ripening of beef, strains V3/3/4/13 and V3/K/3/5 were isolated. Strain V3/3/4/13 exhibited more than 99 % 16S rRNA gene-based similarity to and other members of this group, while isolate V3/K/3/5 was very close to and a number of relatives within the group. Additional comparisons of complete sequences and draft genomes allowed us to place isolate V3/3/4/13 close to DSM 26521.

sp. nov. isolated from var. Rijnsburger, an onion landrace.

The novel, aerobic, Gram-stain-positive, rod-shaped bacterial strain, ZW T2_19, was isolated from an onion sample ( var. Rijnsburger). Analyses of the 16S rRNA gene sequence revealed that ZW T2_19 represented a member of the genus but may represent a novel species of this genus.

sp. nov., isolated from bulk tank raw milk at a German dairy farm.

Here we present the description of a novel species, designated sp. nov., which was isolated from raw milk samples obtained from Germany.

sp. nov. isolated from refrigerated dry-aged beef.

A polyphasic approach was applied to investigate the diversity of microbiota that evolved during cold storage beef ripening. Isolate V4/DAB/S4/2a with a unique BOX-rep-PCR fingerprint profile revealed more than 99 % nucleotide identities upon pairwise comparisons of 16S rDNA sequences from the type strains DSM 101070, DSM 108989, DSM 26521 and DSM 29166, placing it within the / branch of the genus . Additional based comparison revealed DSM 101070 as the nearest relative, with 98.

Genome analysis reveals that the correct name of type strain DSM 22242 is Clavel 2013.

The strain DSM 22242 (=CCUG 57646=NR06) was taxonomically described in 2013 and named as Clavel 2013. In 2018, the name of the strain DSM 22242 was changed to (Clavel 2013) Nouioui 2018 due to taxonomic investigations of the closely related genera and within the phylum . However, the first whole draft genome of strain DSM 22242 was published by our group in 2019.

sp. nov. and sp. nov., isolated from raw milk in Germany.

In this study, nine Gram-negative, motile and rod-shaped bacteria were isolated during a Germany-wide investigation of raw milk microbiota. The strains could be differentiated from their closest relatives by phenotypic and chemotaxonomic characterization and average nucleotide identity (ANIb) values calculated from draft genome assemblies. Strains MBT-1, MBT-8, MBT-9, MBT-10, MBT-11 and MBT-12 were related to the subgroup.

sp. nov., isolated from refrigerated beef.

During a project focusing on the diversity of meat microbiota associated with beef ripening, a strain was isolated exhibiting high 16S rRNA gene sequence similarities (>99 %) to DSM 107652, DSM 29167, DSM 29164 and DSM 18862. Phylogenetic analysis of the complete gene sequences of the isolate V5/DAB/2/5 indicated a separate branch with about 99.0 % nucleotide identities to the closest relatives DSM 107652, DSM 29167 and DSM 29164, while average nucleotide identities (ANIb) calculated from the draft genomes were 94.

Coping with anoxia: a comprehensive proteomic and transcriptomic survey of denitrification.

Ralstonia eutropha H16 is a denitrifying microorganism able to use nitrate and nitrite as terminal electron acceptors under oxygen deprivation. To identify proteins showing an altered expression pattern in response to oxygen supply, R. eutropha cells grown aerobically and anaerobically were compared in a comprehensive proteome and transcriptome approach.

A transcriptional study of acidogenic chemostat cells of Clostridium acetobutylicum--solvent stress caused by a transient n-butanol pulse.

The main product of the anaerobic fermentative bacterium Clostridium acetobutylicum is n-butanol, an organic solvent with severe toxic effects on the cells. Therefore, the identification of the molecular factors related to n-butanol stress constitutes a major strategy for furthering the understanding of the biotechnological production of n-butanol, an important industrial biofuel. Previous reports concerning n-butanol stress in C.

A transcriptional study of acidogenic chemostat cells of Clostridium acetobutylicum--cellular behavior in adaptation to n-butanol.

To gain more insight into the butanol stress response of Clostridium acetobutylicum the transcriptional response of a steady state acidogenic culture to different levels of n-butanol (0.25-1%) was investigated. No effect was observed on the fermentation pattern and expression of typical solvent genes (aad, ctfA/B, adc, bdhA/B, ptb, buk).

Global transcriptional changes of Clostridium acetobutylicum cultures with increased butanol:acetone ratios.

Artificial electron carriers have been widely used to shift the solvent ratio toward butanol in acetone-butanol-ethanol (ABE) fermentation of solventogenic clostridia according to decreased hydrogen production. In this study, first insights on the molecular level were gained to explore the effect of methyl viologen addition to cultures of Clostridium acetobutylicum. Employing batch fermentation in mineral salts medium, the butanol:acetone ratio was successively increased from 2.

Genome-wide gene expression analysis of the switch between acidogenesis and solventogenesis in continuous cultures of Clostridium acetobutylicum.

Clostridium acetobutylicum is able to switch from acidogenic growth to solventogenic growth. We used phosphate-limited continuous cultures that established acidogenic growth at pH 5.8 and solventogenic growth at pH 4.

Transcriptional analysis of catabolite repression in Clostridium acetobutylicum growing on mixtures of D-glucose and D-xylose.

Clostridium acetobutylicum is a strict anaerobic organism that is used for biotechnological butanol fermentation. It ferments various hexoses and pentoses to solvents but prefers glucose presumably using a catabolite repression mechanism. Accordingly during growth on a mixture of D-glucose and D-xylose a typical diauxic growth pattern was observed.