Publications by authors named "Christina Barnes"
Article Synopsis
- - The Neogen® Molecular Detection Assay 2-Salmonella Enteritidis/Salmonella Typhimurium (MDA2-SE/ST) is a fast test aimed at accurately detecting two specific types of Salmonella in poultry samples.
- - The study validated this method through rigorous testing on chicken carcass rinse and raw ground chicken to ensure it functions correctly and meets Performance Tested Methods (PTM) certification standards.
- - Results confirmed that the MDA2-SE/ST method accurately identifies Salmonella Enteritidis and Salmonella Typhimurium, showing high specificity and agreement with traditional reference methods, leading to its approval by the AOAC PTM Program.
Background: The 3M™ Molecular Detection Assay 2 - STEC Gene Screen (stx and eae) method is based on gene amplification by the use of real-time loop-mediated isothermal amplification when used with the 3M Molecular Detection System for the rapid and specific detection of Shiga toxin gene (stx1 and/or stx2) and intimin gene (eae) from Shiga toxin-producing enterohemorrhagic Escherichia coli (STEC) in enriched foods.
Objective: The 3M Molecular Detection Assay 2 - STEC Gene Screen (stx and eae) method was evaluated as a Level 2 method modification to add new matrixes to the certified claim: 25 g fresh raw ground beef (approximately 75% lean), 375 g fresh raw ground pork (approximately 70% lean), 375 g fresh raw poultry parts, and 25 g sprouts.
Methods: Matrix studies were conducted to assess the method's performance compared to the U.
Background: The 3M™ Molecular Detection Assay 2 - STEC Gene Screen (stx) method is based on gene amplification by the use of real-time loop-mediated isothermal amplification when used with the 3M Molecular Detection System for the rapid and specific detection of Shiga toxin gene (stx1 and/or stx2) from Shiga toxin-producing enterohemorrhagic Escherichia coli (STEC) in enriched foods.
Objective: The 3M Molecular Detection Assay 2 - STEC Gene Screen (stx) method was evaluated as a Level 2 method modification to add new matrixes to the certified claim: 25 g fresh raw ground beef (approximately 75% lean), 375 g raw beef trim (approximately 75% lean), 375 g fresh raw ground pork (approximately 70% lean), 375 g fresh raw poultry parts, and 25 g sprouts.
Methods: Matrix studies were conducted to assess the method's performance compared to the US Department of Agriculture Food Safety and Inspection Service Microbiology Laboratory Guidebook, 5C.
Background: The 3M™ Molecular Detection Assay 2 - STEC Gene Screen (stx) method is based on gene amplification by the use of real time loop-mediated isothermal amplification when used with the 3M Molecular Detection System for the rapid and specific detection of Shiga toxin gene (stx1 and/or stx2) from Shiga toxin-producing Escherichia coli (STEC) in enriched foods. The stx assay does not differentiate between stx1 and stx2 but detects the presence of stx1 and/or stx2.
Objective: The 3M Molecular Detection Assay 2 - STEC Gene Screen (stx) method was evaluated for AOAC® Performance Tested MethodsSM certification.
Article Synopsis
- The 3M Molecular Detection Assay 2 - STEC Gene Screen uses gene amplification to quickly identify harmful E. coli strains in food, particularly targeting the Shiga toxin and intimin genes.
- The method underwent rigorous testing for certification, including checking its accuracy against established USDA and FDA guidelines for different food matrices.
- Results showed the assay effectively detected all relevant E. coli strains while maintaining consistent performance across different batches, proving it to be a reliable tool for food safety.
Objective: Adolescents with asthma are influenced by peers and family. The objective was to better understand family social support and test its association with medication adherence, asthma control, and Emergency Department (ED) use.
Methods: This study is a cross-sectional secondary data analysis from a randomized controlled trial with urban adolescents from three U.