Impacts of Fungicide Treatment and Conventional Fertilization Management on the Potato Metabolome ( L.) Evaluated with UPLC-IMS-QToF.

Two field trials were conducted to investigate the influence of fungicide and fertilization management on the potato tubers' metabolome ( L.). Thereby, fungicides and conventional fertilizers were varied in terms of quantities, number and date of applications, physical state, and product composition.

Polar Lipids in Starch-Rich Commodities to be Analyzed with LC-MS-Based Metabolomics-Optimization of Ionization Parameters and High-Throughput Extraction Protocols.

Metabolomics-based approaches are still receiving growing attention with regard to food authenticity testing. Such studies require enormous sample numbers with negligible experimental or analytical variations to obtain statistically reliable results. In this context, an extraction protocol in line with optimized ionization parameters was developed in consideration of potential starch-derived matrix effects focusing on the polar lipids of potatoes.

Metabolomics-Based Approach for the Discrimination of Potato Varieties ( Solanum tuberosum) using UPLC-IMS-QToF.

One hundred eighty-two authentic potato samples ( Solanum tuberosum) of known variety were collected from various German regions in 2016 and 2017. Samples were extracted with a liquid-liquid-extraction protocol that included isopropanol, methanol, and water in order to focus on lipophilic metabolites. The analysis of nonpolar extracts was performed using an UPLC-IMS-QToF-MS system; data sets obtained were evaluated via multivariate data analysis.