Bartonella quintana in cynomolgus monkey (Macaca fascicularis).

We identified a Bartonella quintana strain by polymerase chain reaction amplification, cloning, and sequencing of DNA extracted from lysed erythrocytes and cultured colonies grown from peripheral blood collected from a captive-bred cynomolgus monkey (Macaca fascicularis). This report describes naturally acquired B. quintana infection in a nonhuman primate.

Capnocytophaga sp. isolated from a cat with chronic sinusitis and rhinitis.

A Capnocytophaga sp. was inadvertently isolated from a cat with chronic sinusitis and rhinitis when cytopathic effects were observed in Crandall-Reese feline kidney cells that had been inoculated with oropharyngeal swab samples. Although Capnocytophaga spp.

Investigation of the phylogenetic relationships within the genus Bartonella based on comparative sequence analysis of the rnpB gene, 16S rDNA and 23S rDNA.

A variety of genes and analytical methods have been applied to the study of phylogenetic relationships within the genus Bartonella, but so far the results have been inconsistent. While previous studies analysed single protein-encoding genes, we have analysed an alignment containing the sequences of three important phylogenetic markers, RNase P RNA, 16S rRNA and 23S rRNA, merged by catenation, to determine the phylogenetic relationships within the genus Bartonella. The dataset described here comprises 13 different Bartonella strains, including the seven strains that are known to be human pathogens.

Differentiation of Haemobartonella canis and Mycoplasma haemofelis on the basis of comparative analysis of gene sequences.

Objective: To determine whether Haemobartonella canis and Mycoplasma haemofelis (formerly known as H felis [large form]) can be differentiated by use of comparative analysis of gene sequences.

Sample Population: Blood samples obtained from 3 dogs infected with H canis and 2 cats infected with M haemofelis.

Procedure: The partial 16S rDNA and ribonuclease P RNA (RNase P) genes were amplified, cloned, and sequenced in blood samples obtained from H canis-infected dogs and M haemofelis-infected cats.

Isolation and identification of Mycobacterium kansasii from pleural fluid of a dog with persistent pleural effusion.

A 3-year-old spayed female Whippet was examined for cough and respiratory distress. Lung lobe torsion with pleural effusion was diagnosed, and lung lobectomy was performed. Pleural effusion recurred during the following 27 months; conventional bacteriologic cultures of pleural effusion did not result in bacterial growth.